<i>In vitro</i> Shoot Regeneration and Heterosis studies in Tomato (<i>Solanum lycopersicon</i> Mill.)

Bhushan, Anil; Gupta, Rakesh

doi:10.5958/2229-4473.2017.00097.0

Cited by 3 publications

(2 citation statements)

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“…The effect of low cost gelling agent's viz., isabgol and sago was studied as compared to agar on shoot multiplication in six tomato genotypes [62]. The results revealed isabgol to be the most suitable gelling agent recording maximum shoot multiplication in all the genotypes.…”

Section: Tomatomentioning

confidence: 99%

Low Cost Tissue Culture Technologies in Vegetables: A Review

Naik

Bhushan

Gupta

et al. 2020

IJBcRR

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The demand of vegetable crops is increasing day by day due to changes in consumption patterns, so the need of the hour is to develop technologies that enhance the vegetable production at a rapid rate. Plant Tissue culture is one such remarkable biotechnological tool that has its application in vegetable propagation and improvement, disease elimination, herbicide resistance, salinity tolerance, incorporation of high nutrient content, genetically improved plants and conservation of endangered plant species and in the near future usage of this technology is going to increase further manifold. It is used for production of disease free quality planting material and development of varieties through direct regeneration, anther/ovule culture, somatic embryogenesis etc. or for creation of new variation (organogenesis via callus formation, soma-clonal variation and in vitro mutagenesis). In spite of being a very important and viable non-conventional biotechnological tool, high cost of production of seedlings in vitro remains a major impediment in popularization of this technology. High cost of producing seedlings is due to availability of limited resources, high recurrent costs of consumables for media and lack of awareness, which limits its application only to a few institutions and rich farmers especially in developing countries. Therefore, in order to make this technology a successful and viable option for the farmers, future thrust must be on cost reduction of in vitro seedlings. The components of tissue culture technology such as culture media components, glassware, lighting and water for media preparation can be replaced with low cost alternatives to reduce the overall cost of tissue culture. The usage of alternatives for gelling agent’s like isabgol (potato, tomato, cassava, turmeric, ginger), sago (potato, tomato, turmeric, ginger) cassava starch (potato, cassava, sweet potato) barley starch, phytagel etc. and for carbon sources like table sugar (potato, turmeric, ginger), jaggery, sugarcane juice, cube sugar (bittergord), brown sugar etc have already been documented worldwide. The present paper reviews the work done by researchers around the globe in developing various low cost alternative technologies with focus on vegetable crops.

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Section: Tomatomentioning

confidence: 99%

Low Cost Tissue Culture Technologies in Vegetables: A Review

Naik

Bhushan

Gupta

et al. 2020

IJBcRR

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“…Research on cultivated tomato ( Solanum lycopersicon Mill.) has reported heterosis for adventitious shoot regeneration in terms of maximum shoot multiplication rate [ 17 ]. Significant positive heterosis effects were also observed in some crosses of different wheat genotypes for callus induction and plant regeneration [ 18 ].…”

Section: Introductionmentioning

confidence: 99%

Allele-specific DNA methylation and gene expression during shoot organogenesis in tissue culture of hybrid poplar

Guo,

Feng,

Yang

et al. 2024

Horticulture Research

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Plant tissue regeneration is critical for genetic transformation and genome editing techniques. During the regeneration process, changes in epigenetic modifications accompany the cell fate transition. However, how allele-specific DNA methylation in two haplotypes contributes to the transcriptional dynamics during regeneration remains elusive. Here, we applied an inter-species hybrid poplar (Populus alba × P. glandulosa cv. 84K) as a system to characterize the DNA methylation landscape during de novo shoot organogenesis at allele level. Both direct and indirect shoot organogenesis experienced a reduction in genome-wide DNA methylation. At gene level, non-expressed genes were hypermethylated in comparison to expressed genes. Among the genes exhibiting significant correlations between levels of DNA methylation and gene expression, the expression patterns of 75% genes were negatively correlated with DNA methylation in CG context, whereas the correlation patterns in CHH context were reverse. The allele-biased DNA methylation was consistent during shoot organogenesis, with less than one thousandth of allele-specific methylation regions shifted. Analysis of allele-specific expression revealed that there were only 1909 genes showing phase-dependent allele-biased expression in the regeneration process, among which the allele pairs with greater differences in transcriptional factor binding sites (TFBS) at promoter regions exhibited greater differences in allele expression. Our results indicated a relatively independent transcriptional regulation in two sub genomes during shoot organogenesis, which were contributed by cis-acting genomic and epigenomic variations.

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