<i>In Vitro</i> Susceptibility to Tetracycline and Fluoroquinolones of Japanese Isolates of <i>Coxiella burnetii</i>

Andoh, Masako; Naganawa, Shinji; Yamaguchi, Tsuyoshi; Fukushi, Hideto; Hirai, Katsuya

doi:10.1111/j.1348-0421.2004.tb03476.x

Cited by 7 publications

(6 citation statements)

References 12 publications

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“…It was then applied in another study from our team, which confirmed its excellent reproducibility for MIC determination (20). In recent years, both the IFA and qPCR tests targeting com1 or apoB have been used for C. burnetii AST in various eukaryotic cell lines (21)(22)(23)(24). We recently developed a new method using flow cytometry and specific immunofluorescent probes.…”

Section: Search Strategymentioning

confidence: 79%

From Q Fever to Coxiella burnetii Infection: a Paradigm Change

et al. 2017

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Coxiella burnetii is the agent of Q fever, or "query fever," a zoonosis first described in Australia in 1937. Since this first description, knowledge about this pathogen and its associated infections has increased dramatically. We review here all the progress made over the last 20 years on this topic. C. burnetii is classically a strict intracellular, Gram-negative bacterium. However, a major step in the characterization of this pathogen was achieved by the establishment of its axenic culture. C. burnetii infects a wide range of animals, from arthropods to humans. The genetic determinants of virulence are now better known, thanks to the achievement of determining the genome sequences of several strains of this species and comparative genomic analyses. Q fever can be found worldwide, but the epidemiological features of this disease vary according to the geographic area considered, including situations where it is endemic or hyperendemic, and the occurrence of large epidemic outbreaks. In recent years, a major breakthrough in the understanding of the natural history of human infection with C. burnetii was the breaking of the old dichotomy between "acute" and "chronic" Q fever. The clinical presentation of C. burnetii infection depends on both the virulence of the infecting C. burnetii strain and specific risks factors in the infected patient. Moreover, no persistent infection can exist without a focus of infection. This paradigm change should allow better diagnosis and management of primary infection and long-term complications in patients with C. burnetii infection.

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Section: Search Strategymentioning

confidence: 79%

From Q Fever to Coxiella burnetii Infection: a Paradigm Change

et al. 2017

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“…At the concentrations used (for tigecycline, 0.03 g/ml to 8 g/ml, and for the rest of the antibiotics, 0.25 g/ml to 16 g/ml), the solvents were nontoxic to normal or infected Vero cells. The bacteriostatic and bactericidal effects of antibiotics against C. burnetii were tested by a shell vial assay (1,4,5,6,9,13,16,21). For this purpose, Vero cell monolayers cultured in shell vials were infected with 10-fold serial dilutions of a C. burnetii inoculum to determine the inoculum dilution that resulted in 50% infected Vero cells after 6 days of incubation.…”

mentioning

confidence: 99%

“…Furthermore, a combination of doxycycline with the lysosomotropic agent chloroquine displayed bactericidal activity in vitro, but no definitive clinical data were presented (8,17,18). Quinolones also exhibit in vitro activities against rickettsiae and have produced encouraging clinical results in Q fever patients (1,6,21). Previous work with other bacteria demonstrated that tigecycline specifically inhibits protein synthesis in an in vitro assay system containing the bacterial enzymes required for transcription and translation.…”

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confidence: 99%

Bacteriostatic and Bactericidal Activities of Tigecycline against Coxiella burnetii and Comparison with Those of Six Other Antibiotics

Spyridaki

Psaroulaki

Vranakis

et al. 2009

Antimicrob Agents Chemother

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The present article is a study of the in vitro susceptibility of eight Greek Coxiella burnetii isolates, derived from patients with acute Q fever, and two reference strains of Coxiella burnetii to tigecycline. The bacteriostatic activity of tigecycline was compared with those of six other antibiotics using a shell vial assay. The MICs of the examined antibiotics were as follows: tigecycline ranged from 0.25 to 0.5 g/ml; doxycycline, trovafloxacin, and ofloxacin ranged from 1 to 2 g/ml; linezolid and clarithromycin ranged from 2 to 4 g/ml; and ciprofloxacin ranged from 4 to 8 g/ml. Tigecycline was effective in inhibiting the infection of Vero cells by C. burnetii. No bactericidal activity was observed against C. burnetii at 4 g/ml.

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“…Studies suggest doxycycline, gatifloxacin, levofloxacin, moxifloxacin, and ciprofloxacin are the most effective antibiotics against C. burnetii isolates [14]. The patient initially received moxifloxacin and levofloxacin, but the response was unsatisfactory.…”

Section: Discussionmentioning

confidence: 99%

Acute Q fever pneumonia diagnosed by metagenomic next-generation sequencing

Liu,

Huang,

Liang

et al. 2024

J Infect Dev Ctries

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Introduction: Q fever, a zoonotic disease caused by Coxiella burnetii (C. burnetii), presents diagnostic challenges due to its clinical and radiological nonspecificity, which often mimics community-acquired pneumonia, coupled with the limitations of traditional diagnostic methods. Metagenomic next-generation sequencing (mNGS) has become an indispensable tool in clinical diagnostics for its high-throughput pathogen identification capabilities. Herein, we detail a case of acute Q fever pneumonia diagnosed with mNGS. Case presentation: The patient exhibited symptoms of fever, cough, expectoration, and diarrhea for three days, with the pathogen undetected in initial laboratory assessments. Bronchoscopy and bronchoalveolar lavage (BAL) were conducted, leading to the identification of C. burnetii in the lavage fluid via mNGS. Consequently, the patient was promptly initiated on a treatment regimen of 100 mg doxycycline, administered orally every 12 hours. Results: Post-treatment, the patient's temperature normalized, and a full recovery was observed. The follow-up chest CT scan revealed complete resolution of the right lower lobe consolidation. Conclusions: The clinical presentation of Q fever pneumonia lacks specificity, making diagnosis based solely on symptoms and imaging challenging. mNGS offers a superior alternative for identifying elusive or rarely cultured pathogens.

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In Vitro Susceptibility to Tetracycline and Fluoroquinolones of Japanese Isolates of Coxiella burnetii

Cited by 7 publications

References 12 publications

From Q Fever to Coxiella burnetii Infection: a Paradigm Change

From Q Fever to Coxiella burnetii Infection: a Paradigm Change

Bacteriostatic and Bactericidal Activities of Tigecycline against Coxiella burnetii and Comparison with Those of Six Other Antibiotics

Acute Q fever pneumonia diagnosed by metagenomic next-generation sequencing

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