In VitroSynergism of ConcentratedAllium sativumExtract and Amphotericin B againstCryptococcus neoformans

Davis, Larry E.; Shen, Jinkun; Royer, Robert E.

doi:10.1055/s-2006-959568

Cited by 45 publications

(28 citation statements)

References 3 publications

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“…Davis et al (1994) show an in vitro synergistic fungistatic activity effect of concentrated Allium sativum extract with amB against C. neoformans. Another study also showed an increase in antifungal activity of amB in the presence of acteoside, a phenylethanoid glycoside from Colebrookea oppositifolia (Ali et al, 2011).…”

Section: Resultsmentioning

confidence: 79%

Quercetin and rutin as potential agents antifungal against Cryptococcus spp

et al. 2016

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Amphotericin B is a fungicidal substance that is treatment of choice for most systemic fungal infections affecting as cryptococcosis the immunocompromised patients. However, severe side effects have limited the utility of this drug. The aim of this study was to evaluate the antifungal effect of the combination of amphotericin B with quercetin or rutin and as a protective of citotoxic effect. The antifungal activity to amphotericin B, quercetin and rutin alone and in combination was determined in Candida sp and Cryptococcus neoformans strains. Cytotoxicity test on erythrocytes was performed by spectrophotometric absorbance of hemoglobin. The amphotericin B MIC was reduced when used in combination with quercetin or rutin to C. neoformans ATCC strain and reduced when combined with rutin to a clinical isolate of C. neoformans. In addition, the combination of quercetin with amphotericin B may reduce the toxicity of amphotericin B to red blood cells. Our results suggest that quercetin and rutin are potential agents to combine with amphotericin B in order to reduce the amphotericin dose to lessen side effects and improve antifungal efficacy.Keywords: amphotericin B, quercetin, rutin, Cryptococcus neoformans. Quercetina e rutina: potenciais agentes para terapia antifúngica ResumoA anfotericina B é uma substância fungicida e é o tratamento de escolha para a maioria das infecções fúngicas sistêmicas que afetam os pacientes imunocomprometidos, como a criptococose. No entanto, as severas reações adversas têm limitado a utilização desta droga. O objetivo deste estudo foi avaliar o efeito antifúngico e o potencial efeito protetor de citotoxicidade da combinação de anfotericina B com quercetina ou rutina. A atividade antifúngica de anfotericina B, quercetina e rutina, isoladamente e em combinação foi determinada em cepas de Candida sp e Cryptococcus neoformans. O teste de citotoxicidade em eritrócitos foi realizado por espectrofotometria, através da determinação da absorbância da hemoglobina. A concentração inibitória mínima da anfotericina B foi reduzida quando utilizada em combinação com a quercetina e rutina em C. neoformans ATCC e reduzida quando combinados com rutina em um isolado clínico de C. neoformans. Além disso, a combinação de quercetina com anfotericina B pode reduzir a toxicidade da droga em eritrócitos. Os resultados sugerem que quercetina e rutina são potenciais agentes para combinação com anfotericina B, a fim de reduzir a dose de anfotericina, diminuindo os efeitos colaterais e melhorando sua eficácia antifúngica.Palavras-chave: anfotericina B, quercetina, rutina, Cryptococcus neoformans.

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Section: Resultsmentioning

confidence: 79%

Quercetin and rutin as potential agents antifungal against Cryptococcus spp

et al. 2016

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“…Minimum growth inhibitory concentrations (MICs) of AmB, AmBDs and MC12 were determined by the twofold broth dilution method, applying the chequerboard technique (Davis et al, 1994;Tanaka et al, 2000). Cells were grown overnight in YPD medium containing 1 % yeast extract (Difco Laboratories), 2 % bacto-peptone (Difco Laboratories), and 2 % D-glucose, with vigorous shaking at 30 uC.…”

Section: Methodsmentioning

confidence: 99%

Visualization analysis of the vacuole-targeting fungicidal activity of amphotericin B against the parent strain and an ergosterol-less mutant of Saccharomyces cerevisiae

et al. 2013

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Here, we sought to investigate the vacuole-targeting fungicidal activity of amphotericin B (AmB) in the parent strain and AmB-resistant mutant of Saccharomyces cerevisiae and elucidate the mechanisms involved in this process. Our data demonstrated that the vacuole-targeting fungicidal activity of AmB was markedly enhanced by N-methyl-N0-dodecylguanidine (MC12), a synthetic analogue of the alkyl side chain in niphimycin, as represented by the synergy in their antifungal activities against parent cells of S. cerevisiae. Indifference was observed only with Derg3 cells, indicating that the replacement of ergosterol with episterol facilitated their resistance to the combined lethal actions of AmB and MC12. Dansyl-labelled amphotericin B (AmB-Ds) was concentrated into normal rounded vacuoles when parent cells were treated with AmB-Ds alone, even at a non-lethal concentration. The additional supplementation of MC12 resulted in a marked loss of cell viability and vacuole disruption, as judged by the fluorescence from AmB-Ds scattered throughout the cytoplasm. In Derg3 cells, AmB-Ds was scarcely detected in the cytoplasm, even with the addition of MC12, reflecting its failure to normally incorporate across the plasma membrane into the vacuole. Thus, this study supported the hypothesis that ergosterol is involved in the mobilization of AmB into the vacuolar membrane so that AmB-dependent vacuole disruption can be fully enhanced by cotreatment with MC12.

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“…MICs of PMB and allicin were determined against Escherichia coli IFO 3545, Bacillus subtilis IFO 3007, S. cerevisiae W303-1A, C. albicans IFO 1061, Aspergillus fumigatus IFO 5840, and A. niger ATCC 6275 by 2-fold broth dilution method applying the checkerboard technique [19]. Cells of bacterial strains were grown overnight in 3.0% nutrient broth (Nissui Co., Tokyo, Japan) with vigorous shaking at 30°C, and diluted with the same medium to 10 6 cells/ml.…”

Section: Measurement Of Cell Growth and Viabilitymentioning

confidence: 99%

Amplification of Vacuole-targeting Fungicidal Activity of Antibacterial Antibiotic Polymyxin B by Allicin, an Allyl Sulfur Compound from Garlic

et al. 2007

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A cationic antibacterial peptide, polymyxin B (PMB), was evaluated as an antifungal antibiotic against various yeasts and filamentous fungi when used in combination with allicin, an allyl sulfur compound from garlic. Allicin was not lethal but could markedly amplify the fungicidal activity of PMB, which was weakly detected with the increase in the plasma membrane permeability in Saccharomyces cerevisiae. Their combined actions caused a dynamic structural damage to the yeast vacuole as judged by the disappearance of its swollen spherical architecture. The vacuole-targeting activity of PMB was similarly amplified in medium with t-butyl hydroperoxide as a substitute for the action of allicin. These findings suggest that the allicin-mediated lipoperoxide production in fungal plasma membrane is the cause of the enhancement in the cellular uptake of PMB as well as its action against the vacuole.Keywords polymyxin B, allicin, Saccharomyces cerevisiae, vacuole, plasma membrane IntroductionPolymyxin B (PMB, Fig. 1) is a decapeptide antibiotic characterized by a heptapeptide ring containing four 2,4-diaminobutyric acids. An additional peptide chain covalently bound to the cyclic peptide carries an aliphatic chain attached to the peptide through an amide bond. PMB is bactericidal to almost all Gram-negative bacteria at relatively low concentrations [1]. The antibiotic is known to disrupt a permeability barrier of the bacterial outer membrane by forming a complex with lipopolysaccharide [2,3]. Therefore, PMB is expected to increase the

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In VitroSynergism of ConcentratedAllium sativumExtract and Amphotericin B againstCryptococcus neoformans

Cited by 45 publications

References 3 publications

Quercetin and rutin as potential agents antifungal against Cryptococcus spp

Quercetin and rutin as potential agents antifungal against Cryptococcus spp

Visualization analysis of the vacuole-targeting fungicidal activity of amphotericin B against the parent strain and an ergosterol-less mutant of Saccharomyces cerevisiae

Amplification of Vacuole-targeting Fungicidal Activity of Antibacterial Antibiotic Polymyxin B by Allicin, an Allyl Sulfur Compound from Garlic

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