In VitroSynergistic Effect of Curcumin in Combination with Third Generation Cephalosporins against Bacteria Associated with Infectious Diarrhea

Abstract

Diarrhea is one of the leading causes of morbidity and mortality in humans in developed and developing countries. Furthermore, increased resistance to antibiotics has resulted in serious challenges in the treatment of this infectious disease worldwide. Therefore, there exists a need to develop alternative natural or combination drug therapies. The aim of the present study was to investigate the synergistic effect of curcumin-1 in combination with three antibiotics against five diarrhea causing bacteria. The antibacterial activity of curcumin-1 and antibiotics was assessed by the broth microdilution method, checkerboard dilution test, and time-kill assay. Antimicrobial activity of curcumin-1 was observed against all tested strains. The MICs of curcumin-1 against test bacteria ranged from 125 to 1000 μg/mL. In the checkerboard test, curcumin-1 markedly reduced the MICs of the antibiotics cefaclor, cefodizime, and cefotaxime. Significant synergistic effect was recorded by curcumin-1 in combination with cefotaxime. The toxicity of curcumin-1 with and without antibiotics was tested against foreskin (FS) normal fibroblast and no significant cytotoxicity was observed. From our result it is evident that curcumin-1 enhances the antibiotic potentials against diarrhea causing bacteria in in vitro condition. This study suggested that curcumin-1 in combination with antibiotics could lead to the development of new combination of antibiotics against diarrhea causing bacteria.

Antibacterial Action of Curcumin against Staphylococcus aureus: A Brief Review

“…The activity was time- and dose-dependent, and 100% killing was achieved at 50  μ M (equivalent to 18.42  μ g/mL) after 2 hr exposure [30]. In contrast, Sasidharan et al [41] showed that the same compound had a MIC of 250  μ g/mL against S. aureus , which is comparable to the native curcumin [8, 9]. In an in vivo mouse model, administration of 100 mg/kg curcumin was shown to protect the mice infected with both MSSA and MRSA from pneumonia by targeting the α -hemolysin (HIa) protein of S. aureus [40].…”

“…Upper panel shows the structure of curcumin-1 [30, 41] while the lower panel shows the metal complexes of curcumin, indium curcumin [42, 43]. The chemical structures above were drawn using a free online tool, ChemWriter (http://chemwriter.com/).…”

Antibacterial synergy of curcumin with antibiotics against biofilm producing clinical bacterial isolates

“…[ 5 ] Recently, its antibacterial action is found to be synergistic with several antibiotics. [ 6 7 ] However, there is limited research data on this synergistic antibacterial action of curcumin, especially against biofilm producing organisms to support its benefits in biofilm-associated infections. We conducted this study to determine the in vitro synergy of curcumin with antibiotics against biofilm producing clinical bacterial isolates.…”

Antibacterial non-woven nanofibers of curcumin acrylate oligomers

“…3). 35 However, the CUR used in our studies recorded a MIC of 2 mg mL À1 . 34 CUR is antibacterial, and according to the literature, the minimum inhibitory concentration (MIC) ranges between 0.25 and 0.5 mg mL À1 .…”