<i>In vivo</i> bioluminescence tomography with a blocking‐off finite‐difference  method and MRI/CT coregistration

Klose, Alexander D.; Beattie, Bradley J.; Dehghani, Hamid; Vider, Jelena; Le, Carl; Ponomarev, Vladimir; Blasberg, Ronald G.

doi:10.1118/1.3273034

Cited by 74 publications

(64 citation statements)

References 40 publications

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“…31 and 32) or MRI. 33,34 The CT or MRI images can be segmented into different organs which are then assigned corresponding optical property values. Consequently, the anatomical and optical information obtained from CT or MRI serves as a prior knowledge for the BLT reconstruction.…”

Section: Discussionmentioning

confidence: 99%

Systematic calibration of an integrated x‐ray and optical tomography system for preclinical radiation research

et al. 2015

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Purpose: The cone beam computed tomography (CBCT) guided small animal radiation research platform (SARRP) has been developed for focal tumor irradiation, allowing laboratory researchers to test basic biological hypotheses that can modify radiotherapy outcomes in ways that were not feasible previously. CBCT provides excellent bone to soft tissue contrast, but is incapable of differentiating tumors from surrounding soft tissue. Bioluminescence tomography (BLT), in contrast, allows direct visualization of even subpalpable tumors and quantitative evaluation of tumor response. Integration of BLT with CBCT offers complementary image information, with CBCT delineating anatomic structures and BLT differentiating luminescent tumors. This study is to develop a systematic method to calibrate an integrated CBCT and BLT imaging system which can be adopted onboard the SARRP to guide focal tumor irradiation. Methods: The integrated imaging system consists of CBCT, diffuse optical tomography (DOT), and BLT. The anatomy acquired from CBCT and optical properties acquired from DOT serve as a priori information for the subsequent BLT reconstruction. Phantoms were designed and procedures were developed to calibrate the CBCT, DOT/BLT, and the entire integrated system. Geometrical calibration was performed to calibrate the CBCT system. Flat field correction was performed to correct the nonuniform response of the optical imaging system. Absolute emittance calibration was performed to convert the camera readout to the emittance at the phantom or animal surface, which enabled the direct reconstruction of the bioluminescence source strength. Phantom and mouse imaging were performed to validate the calibration. Results: All calibration procedures were successfully performed. Both CBCT of a thin wire and a euthanized mouse revealed no spatial artifact, validating the accuracy of the CBCT calibration. The absolute emittance calibration was validated with a 650 nm laser source, resulting in a 3.0% difference between simulated and measured signal. The calibration of the entire system was confirmed through the CBCT and BLT reconstruction of a bioluminescence source placed inside a tissue-simulating optical phantom. Using a spatial region constraint, the source position was reconstructed with less than 1 mm error and the source strength reconstructed with less than 24% error. Conclusions: A practical and systematic method has been developed to calibrate an integrated x-ray and optical tomography imaging system, including the respective CBCT and optical tomography system calibration and the geometrical calibration of the entire system. The method can be modified and adopted to calibrate CBCT and optical tomography systems that are operated independently or hybrid x-ray and optical tomography imaging systems. C 2015 American Association of Physicists in Medicine. [http://dx

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Section: Discussionmentioning

confidence: 99%

Systematic calibration of an integrated x‐ray and optical tomography system for preclinical radiation research

et al. 2015

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“…For steady-state FMT with point excitation sources, the following coupled SP 3 equations have been extensively used to depict the photon propagation 9,26 :…”

Section: Methods 21 Sp 3 Modelmentioning

confidence: 99%

Effective and robust approach for fluorescence molecular tomography based on CoSaMP and SP3 model

Guo

et al. 2016

J. Innov. Opt. Health Sci.

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Fluorescence molecular tomography (FMT) allows the detection and quanti¯cation of various biological processes in small animals in vivo, which expands the horizons of pre-clinical research and drug development. E±cient three-dimensional (3D) reconstruction algorithm is the key to accurate localization and quanti¯cation of°uorescent target in FMT. In this paper, 3D reconstruction of FMT is regarded as a sparse signal recovery problem and the compressive sampling matching pursuit (CoSaMP) algorithm is adopted to obtain greedy recovery of°uorescent signals. Moreover, to reduce the modeling error, the simpli¯ed spherical harmonics approximation to the radiative transfer equation (RTE), more speci¯cally SP 3 , is utilized to describe light propagation in biological tissues. The performance of the proposed reconstruction method is thoroughly evaluated by simulations on a 3D digital mouse model by comparing it with three representative greedy methods including orthogonal matching pursuit (OMP), stagewise OMP (StOMP), and regularized OMP (ROMP). The CoSaMP combined with SP 3 shows an improvement in reconstruction accuracy and exhibits distinct advantages over the comparative algorithms in multiple targets resolving. Stability analysis suggests that CoSaMP is robust to noise and performs stably with reduction of measurements. The feasibility and reconstruction accuracy of the proposed method are further validated by phantom experimental data.

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“…18 Some studies have proved that the SP N models can e±ciently improve the imaging accuracy, e.g., BLT, FMT. [19][20][21] Further, some hybrid models based on the SP N have also been proposed to exhibit the applicability in optical imaging. 22,23 However, SP N models do not converge to the exact RTE approximation when N !…”

Section: Introductionmentioning

confidence: 99%

Performance evaluation of the simplified spherical harmonics approximation for cone-beam X-ray luminescence computed tomography imaging

Zhang

Geng

Chen

et al. 2017

J. Innov. Opt. Health Sci.

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As an emerging molecular imaging modality, cone-beam X-ray luminescence computed tomography (CB-XLCT) uses X-ray-excitable probes to produce near-infrared (NIR) luminescence and then reconstructs three-dimensional (3D) distribution of the probes from surface measurements. A proper photon-transportation model is critical to accuracy of XLCT. Here, we presented a systematic comparison between the common-used Monte Carlo model and simpli¯ed spherical harmonics (SP N ). The performance of the two methods was evaluated over several main spectrums using a known XLCT material. We designed both a global measurement based on the cosine similarity and a locally-averaged relative error, to quantitatively assess these methods. The results show that the SP 3 could reach a good balance between the modeling accuracy and computational e±ciency for all of the tested emission spectrums. Besides, the SP 1 (which is equivalent to the di®usion equation (DE)) can be a reasonable alternative model for emission wavelength over 692 nm. In vivo experiment further demonstrates the reconstruction performance of the SP 3 and DE. This study would provide a valuable guidance for modeling the photon-transportation in CB-XLCT.

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In vivo bioluminescence tomography with a blocking‐off finite‐difference method and MRI/CT coregistration

Abstract: The authors demonstrated on in vivo examples that the combination of anatomical coregistration, accurate optical tissue properties, multispectral acquisition, and a blocking-off FD-SP3 solution of the radiative transfer model significantly improves the accuracy of the BLT reconstructions.

Cited by 74 publications

References 40 publications

Systematic calibration of an integrated x‐ray and optical tomography system for preclinical radiation research

Systematic calibration of an integrated x‐ray and optical tomography system for preclinical radiation research

Effective and robust approach for fluorescence molecular tomography based on CoSaMP and SP3 model

Performance evaluation of the simplified spherical harmonics approximation for cone-beam X-ray luminescence computed tomography imaging

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