In VivoCloning of Arylsulfatase-Tyramine Oxidase Genes inrecStrains ofKlebsiella aerogenes

Intergeneric Transfer of the Pullulanase Gene betweenKlebsiella aerogenesandEscherichia colibyIn VivoGenetic Manipulation

“…18 ) Levels ofpullulanase in the·conjugant strain of K. aerogenes was increased about two to three-fold. We have observed similar levels of arylsulfatase production when the enzyme was cloned in vivo by using RP4: : Mu cts, 7) since the copy number of RP4 is one to three per chromosome. 19) Part of the transfer of the pul gene might be due to formation of a RP4: : Mu prime, 4,6,7,13) although the transposition of Mu into the plasmid occurred through recA-independent events.…”

“…We have observed similar levels of arylsulfatase production when the enzyme was cloned in vivo by using RP4: : Mu cts, 7) since the copy number of RP4 is one to three per chromosome. 19) Part of the transfer of the pul gene might be due to formation of a RP4: : Mu prime, 4,6,7,13) although the transposition of Mu into the plasmid occurred through recA-independent events. 20 ) We attempted to isolate such recombinant plasmids from the, transconjugant strains of E. coli (data not shown).…”