In vivo decolourization of the polymeric dye poly R‐478 by corncob cultures of Phanerochaete chrysosporium

Abstract

In this paper, the in vivo decolourization of the polymeric dye Poly R-478 by semi-solid-state cultures of Phanerochaete chrysosporiurn BKM-F-1767 (ATCC 24725) was investigated, employing corncob as a support. In order to stimulate the ligninolytic system of the fungus, the cultures were supplemented with veratryl alcohol (2 mM) or manganese (IV) oxide (1 g/l).Maximum manganese-dependent peroxidase (MnP) and lignin peroxidase (Lip) activities of around 2,OOO U/l and 400 U/l were attained by the former, whereas the activities reached by the latter were of about 1,500 U/1 and 200 U/l, respectively. Furthermore. laccase activity (around 150 U/l) was only detected in manganese (IV) oxide supplemented cultures. The polymeric dye Poly R-478 (0.02 w/v) was added to three-day-old cultures. A percentage of biological decolourization of about 85% was achieved using cultures Supplemented with veratryl alcohol, whereas MnOl cultures showed a rather lower percentage of around 58% after nine days of dye incubation. Moreover, a correlation between MnP activity and Poly R-478 decolourization could be observed, indicating that this enzyme is mainly responsible for dye degradation. In the present work, the in vivo decolourizing capability of the ligninolytic complex secreted by P. chrysosporium was investigated under the above-mentioned cultivation conditions, employing a model compound, such as the polymeric dye Poly R-478.

Competition strategies for the decolorization of a textile‐reactive dye with the white‐rot fungi Trametes versicolor under non‐sterile conditions

“…In experiments with P. chrysosporium, Rodriguez Couto et al (2000) used corn cobs as solid support material with glucose as the auxiliary substrate to successfully decolorize the dye Poly-R-478. They found a 30% weight-loss in the corn cobs and an increase in reducing sugars at the end of their experiments, leading them to conclude that the corn cobs were not used just as support but also as carbon and energy sources, similarly to the results found here.…”

“…Fungal growth in the natural habitat on lignocellulosic solids, e.g., crop residues or wood wastes, is comparatively slow but selective for fungi with lignolytic extracellular enzymes (Eggert, 1994). Sterilized ground corn cobs, for example, were found to be an effective carrier for P. chrysosporium in the decolorization of the polymeric anthraquinone dye Poly R-478 (Rodriguez Couto et al, 2000).…”

Synthesis of calix[6]arene based XAD‐4 material for the removal of reactive blue 19 from aqueous environments

“…8,9 As we know, during the dyeing process, a huge quantity of water is used for dyeing, fixing, and washing purposes. 13,14 The presence of these colored bodies causes some chemical reactions, such as oxidation and hydrolysis, in the wastewater phase that may cause eutrophication, which can reduce the light penetration and create derogatory effects on photosynthesis. 10,11 The waste effluent of dyeing and textile industries is usually a complex mixture of a variety of chemicals ranging from unreacted dyes to auxiliary chemicals.…”

“…This contamination brings about considerable nonaesthetic pollution, and the presence of these dyes in trace quantities, that is, below 1 mg/L, is clearly visible and is considered as toxic to human and aquatic life. 13,14 The presence of these colored bodies causes some chemical reactions, such as oxidation and hydrolysis, in the wastewater phase that may cause eutrophication, which can reduce the light penetration and create derogatory effects on photosynthesis. 15 By virtue of their aromatic nature, most of the dye molecules are resistant to biological and chemical degradation.…”