<i>In vivo</i>deep tissue imaging using wavefront shaping optical coherence tomography

Yu, Hyeonseung; Lee, Peter; Lee, KyeoReh; Jang, Jaeduck; Lim, JaeGuyn; Jang, Wooyoung; Jeong, Yong Hoon; Park, YongKeun

doi:10.1117/1.jbo.21.10.101406

Cited by 26 publications

(22 citation statements)

References 38 publications

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“…Presently, there are few methodologies available to evaluate deep tissue integrity and further validation is required. Fluorescent microangiography, hyperspectral imaging, optical coherence tomography, ultrasound, and nerve mapping are technologies being applied to select studies by only a few investigators . As payors demand higher levels of and broader evidence of clinical benefit in wound healing, as well as data that predict durability of healing, additional objective validated methods to determine wound healing endpoints will be required in trial design.…”

Section: A Standardized Methodology To Assess the Healing Outcomes Ofmentioning

confidence: 99%

Defining complete wound closure: Closing the gap in clinical trials and practice

Gould

2019

Wound Repair Regeneration

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We investigate how wound closure is determined in recent randomized controlled wound trials and real-world studies, identify solutions to the current limitations of wound assessment, and propose a standard methodology to define and assess wound healing in research. We searched PubMed for randomized clinical trials using the terms "complete wound closure" and "wound healing rate" and for real-world studies using the terms "real-world wound healing," "real-world wound data," and "wound registries" dating from March 2010 through March 2018. We selected studies that had "complete wound closure" or "healed wound" as an endpoint. Sixty-five trial articles and 10 real-world articles met our criteria, from which we extracted the wound type studied, definition of healed wound used, wound assessment method, the number of weeks assessed, the number of wounds, and the percent of healed wounds in the study group(s) and control group. There were 7,194 trial wounds included. The most common definition of healing used by 26 studies (40.6%) was complete/full/100% (re)epithelialization or closure without discharge, drainage/scab, and/or dressing. Fiftytwo studies (81.2%) used blinded wound assessment, and at least 10 studies (15.6%) used blinded adjudication. The real-world studies analyzed more than 901,396 wounds. Only three studies (33.3%) defined a healed/closed wound, two of which used "complete epithelialization." Eight studies (88.9%) did not define the wound assessment method; none indicated a blinded assessment. We support the Food and Drug Administration definition: 100% reepithelialization of the wound surface with no discernable exudate and without drainage or dressing, confirmed at two visits 2 weeks apart, and we recommend blinded adjudication for wound assessment. The widespread adoption of a standard wound healing definition and assessment method in wound care research would allow for stronger comparisons of treatment effects across studies to improve the evidence base and strengthen the treatment decisionmaking process in clinical practice.

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Section: A Standardized Methodology To Assess the Healing Outcomes Ofmentioning

confidence: 99%

Defining complete wound closure: Closing the gap in clinical trials and practice

Gould

2019

Wound Repair Regeneration

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“…Another possible application area is optical coherence tomography (OCT) in ophthalmology where the backscattered light from retina within rather “clean” eye can serve as feedback signal. Dedicated wave-front correction techniques improving the penetration depth and image quality in OCT have already been shown by Fiolka et al [ 46 ] and Yu et al [ 47 ]. These works can be extended to achieve light sheet type illumination as well.…”

Section: Discussionmentioning

confidence: 99%

Dynamic light sheet generation and fluorescence imaging behind turbid media

Schneider¹,

Aegerter²

2018

J. Eur. Opt. Soc.-Rapid Publ.

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BackgroundLight sheet microscopy became a popular tool allowing fast imaging with reduced out of focus light. However, when light penetrates turbid media such as biological tissues, multiple scattering scrambles the illumination into a speckle pattern and severely challenges conventional fluorescence imaging with focused light or with a light sheet. In this article, we present generation of light sheet type illumination patterns despite scattering.MethodsWe optimize the wave-front of the incoming light to transform the speckle pattern behind the scattering layer into a light sheet within the region of interest. We utilize a fast spatial light modulator for phase modulation and a genetic optimization algorithm. The light pattern behind the scattering layer is detected via a clear detection path and acts as a feedback signal for the algorithm.ResultsWe enabled homogenous light sheet illumination behind turbid media and enhanced the signal of fluorescent beads selectively at the desired focal plane up to eight times on average. The technique is capable to compensate the dynamic changes of the speckle pattern as well, as shown on samples consisting of living drosophila pupae.ConclusionOur technique shows that not only single foci, but also a homogenous light sheet illumination can directly be created and maintained behind static and dynamic scattering media. To make the technique suitable for common biological settings, where the detection path is turbid as well, a fluorescent probe can be used to provide the feedback signal.Electronic supplementary materialThe online version of this article (10.1186/s41476-018-0074-z) contains supplementary material, which is available to authorized users.

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“…Yet, deep tissue imaging toward applications in dermatology or gastroenterology is technically challenging due to multiple light scattering (Yu et al 2015). There are several attempts to overcome multiple light scattering including optical coherence tomography using wavefront shaping Park et al 2018c;Yu et al 2014;Yu et al 2016) or accumulating single scattering light in deep tissue imaging .…”

Section: Discussionmentioning

confidence: 99%

Holotomography: refractive index as an intrinsic imaging contrast for 3-D label-free live cell imaging

Kim

Lee

et al. 2017

Preprint

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Live cell imaging provides critical information in the investigation of cell biology and related pathophysiology. Refractive index (RI) can serve as intrinsic optical imaging contrast for 3-D label-free and quantitative live cell imaging, and provide invaluable information to understand various dynamics of cells and tissues for the study of numerous fields. Recently significant advances have been made in imaging methods and analysis approaches utilizing RI, which are now being transferred to biological and medical research fields, providing novel approaches to investigate the pathophysiology of cells. To provide insight how RI can be used as an imaging contrast for bioimaging, here we provide the basic principle of RI-based imaging techniques and summarize recent progress on applications, ranging from microbiology, hematology, infectious diseases, hematology, and histopathology.

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In vivodeep tissue imaging using wavefront shaping optical coherence tomography

Cited by 26 publications

References 38 publications

Defining complete wound closure: Closing the gap in clinical trials and practice

Defining complete wound closure: Closing the gap in clinical trials and practice

Dynamic light sheet generation and fluorescence imaging behind turbid media

Holotomography: refractive index as an intrinsic imaging contrast for 3-D label-free live cell imaging

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