In vivo experimental model of human gingival mucosa using immunodeficient mice

Tsukinoki, Keiichi; Miyoshi, Yoshiko; Aoki, Takayuki; Karakida, Kazunari; Ohta, Yoshihisa; Kaneko, Akihiro; Ueyama, Y; Watanabe, Yoshihisa

doi:10.1111/j.1600-0765.2006.00947.x

Cited by 3 publications

(7 citation statements)

References 30 publications

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“…The in vivo evaluation of artificial oral mucosa by implantation in nude mice is a realistic biological model that has been extensively used by several researchers in order to study the in vivo behavior of bioengineered human oral tissues (25,28,29,37). In this regard, the results of this work revealed that the profile of cytokeratin expression was different when the model of artificial oral mucosa was evaluated in vitro and then implanted in vivo in nude mice.…”

Section: Discussionmentioning

confidence: 99%

In vitro and in vivo cytokeratin patterns of expression in bioengineered human periodontal mucosa

Garzón

Sánchez-Quevedo

Moreu

et al. 2009

J of Periodontal Research

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Section: Discussionmentioning

confidence: 99%

In vitro and in vivo cytokeratin patterns of expression in bioengineered human periodontal mucosa

Garzón

Sánchez-Quevedo

Moreu

et al. 2009

J of Periodontal Research

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“…Thus, the transplanted grafts preserved human rather than mouse characteristics. Tsukinoki et al [27] found that grafts in nu/scid mice maintained morphological and differentiation characteristics. The authors did not detect expression of CD34 in nu/scid mice [27], but CD34 expression was noted in endothelial cells of grafts into nu/nu mice.…”

Section: Discussionmentioning

confidence: 99%

“…Tsukinoki et al [27] found that grafts in nu/scid mice maintained morphological and differentiation characteristics. The authors did not detect expression of CD34 in nu/scid mice [27], but CD34 expression was noted in endothelial cells of grafts into nu/nu mice. In the present study, endothelial cells may not have entirely converted to mouse characteristics, because the time that had elapsed after transplantation was shorter than that in the Tsukinoki et al study.…”

Section: Discussionmentioning

confidence: 99%

“…We modified the transplantation method of Tsukinoki et al [27]. The collected tissue was immediately transferred to a mouse on a clean bench as follows.…”

Section: Methodsmentioning

confidence: 99%

“…The native condition of human mucosal tissue was attenuated by this phenomenon. Tsukinoki et al [27] established a transplantation technique for normal human oral mucosa that does not exhibit cystic changes using immunodeficient nu/scid mice, which were derived from a cross of nu/nu mice and scid/scid mice [9, 26]. However, the transplantation of gingival tissue from periodontal diseases has yet to be established in common immunodeficient nu/nu or scid/scid mice.…”

Section: Introductionmentioning

confidence: 99%

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Induction of β-Defensin Expression by Porphyromonas gingivalis-Infected Human Gingival Graft Transplanted in nu/nu Mouse Subdermis

Kamata

Saruta

et al. 2013

Acta Histochem. Cytochem

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It is important to understand the onset of periodontal disease in terms of bacterial infection and host factors. Host-bacteria interactions can be elicited in human cultured cells and animal models, but these models provide only limited biological information about human host reactions against bacterial attacks. Development of an in vivo model using human gingival tissue is needed. We established an in vivo model using nu/nu mice and evaluated host defense following bacterial infection in human gingiva. Human gingival samples were collected from periodontitis patients and transplanted in nu/nu mouse subdermis. After 2 weeks, human characteristics were confirmed by positive immunohistochemical reactions for human-specific markers. We used this model to investigate human β-defensin-2 (hBD-2), an antimicrobial peptide that contributes to initial defense against bacterial invasion. Using real-time polymerase chain reaction, in situ hybridization, and immunohistochemistry, we investigated whether hBD-2 expression was induced in human gingiva as a response to Porphyromonas gingivalis as a periodontal pathogen. Two hours after infection with bacteria, we detected increased expression of hBD-2 mRNA, which was localized in the epithelium of human gingiva. Using our in vivo model, we concluded that increased hBD-2 may play an important role in early defense from bacterial infection in human gingival epithelium.

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Human β-defensin-2 and interleukin-1β expression in response to Porphyromonas gingivalis challenge in mice transplanted with periodontitic human gingiva

Shimizu

Kamata

et al. 2017

Microbial Pathogenesis

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Inter-individual variability in the host response contributes significantly to expression of periodontal disease. Thus, research into the human host response is considered important in the analysis of periodontal disease. Human β-defensin-2 (hBD-2) is typically produced by epithelial tissues after stimulation with microorganisms and inflammatory mediators, and it contributes to the initial defense in the innate immune response. However, hBD-2 expression in response to infection has not been investigated in human gingival tissue with periodontitis. We examined the response to Porphyromonas gingivalis in an established in vivo model of human gingival grafts with various degrees of periodontitis. We also investigated the expression profile of interleukin-1β (IL-1β). Gingival tissues were collected from 40 patients with chronic periodontitis (21 with slight-to-moderate disease, 19 with severe disease) during tooth extraction or periodontal surgery. These tissues were transplanted subcutaneously into nu/nu mice. We used real-time PCR to compare the expression of hBD-2 and IL-1β. In slight-to-moderate chronic periodontitis, hBD-2 expression was significantly higher in the stimulated group than in the non-stimulated group (p < 0.05), but there was no significant increase in the group with severe chronic periodontitis. IL-1β expression did not differ between groups. Increased expression of hBD-2 and IL-1β was associated with slight-to-moderate periodontitis (p < 0.05), and there was a significant relationship between decreased hBD-2 and IL-1β expression and severe periodontitis (p < 0.05). The initial expression profile of hBD-2 in P. gingivalis infection differs according to the severity of periodontitis. In addition, changes in hBD-2 and IL-1β expression may be important in the progression of periodontitis.

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In vivo experimental model of human gingival mucosa using immunodeficient mice

Abstract: The present study revealed that the novel technique of transplantation of human gingival mucosa in nu/scid mice may serve as an in vivo experimental model of periodontal disease.

Cited by 3 publications

References 30 publications

In vitro and in vivo cytokeratin patterns of expression in bioengineered human periodontal mucosa

In vitro and in vivo cytokeratin patterns of expression in bioengineered human periodontal mucosa

Induction of β-Defensin Expression by <i>Porphyromonas gingivalis</i>-Infected Human Gingival Graft Transplanted in <i>nu/nu</i> Mouse Subdermis

Human β-defensin-2 and interleukin-1β expression in response to Porphyromonas gingivalis challenge in mice transplanted with periodontitic human gingiva

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