In vivo imaging of hepatocellular nitric oxide using a hepatocyte-targeting fluorescent sensor

Zhang, Peisheng; Tian, Yong; Liu, Hui; Ren, Junyu; Wang, Hong; Zeng, Rongjin; Long, Yunfei; Chen, Jian

doi:10.1039/c8cc03240h

Cited by 104 publications

(38 citation statements)

References 55 publications

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“…Due to its high sensitivity compared to other imaging modalities, the fluorescence imaging technique is very important in current molecular imaging studies. 38,39 Although the manganese element quenches the emission light of porphyrin in fHMNM, 27 as porphyrin is a modifiable material, manganese was easily replaced by…”

Section: In Vitro Fluorescence Imagingmentioning

confidence: 99%

Multifunctional Hf/Mn-TCPP Metal-Organic Framework Nanoparticles for Triple-Modality Imaging-Guided PTT/RT Synergistic Cancer Therapy

Bao¹,

Zu²,

Wang³

et al. 2020

IJN

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Background: Recent studies have validated and confirmed the great potential of nanoscale metal-organic framework (NMOF) in the biomedical field, especially in improving the efficiency of cancer diagnosis and therapy. However, most previous studies only utilized either the metal cluster or the organic ligand of the NMOF for cancer treatments and merely reported limited theranostic functions, which may not be optimized. As a highly designable and easily functionalized material, prospective rational design offers a powerful way to extract the maximum benefit from NMOF for cancer theranostic applications. Materials and Methods: A NMOF based on hafnium (Hf) cluster and Mn(III)-porphyrin ligand was rational designed and synthesized as a high-performance multifunctional theranostic agent. The folic acid (FA) was modified on the NMOF surface to enhance the cancer targeting efficacy. The proposed "all-in-one" FA-Hf-Mn-NMOF (fHMNM) was characterized and identified using various analytical techniques. Then, in vitro and in vivo studies were performed to further explore the effects of fHMNM both as the magnetic resonance imaging (MRI)/computed tomography (CT)/photoacoustic imaging (PAI) contrast agent and as the photothermal therapy (PTT)/radiotherapy (RT) agent. Results: A tumour targeting multifunctional fHMNM was successfully synthesized with high performance for MRI/CT/PAI enhancements and image-guided PTT/RT synergistic therapy properties. Compared with the current clinical CT and MR contrast agents, the X-ray attenuation and T 1 relaxation rate of this integrated nanosystem increased 1.7-fold and 3-5-fold, respectively. More importantly, the catalase-like Mn(III)-porphyrin ligand can decompose H 2 O 2 into O 2 in tumour microenvironments to improve the synergistic treatment efficiency of PTT and RT. Significant tumour growth inhibition was achieved in mouse cancer models without obvious damage to the other organs. Conclusion: This work highlights the potential of fHMNM as an easily designable material for biomedical applications, could be an effective tool for in vivo detection and subsequent treatment of tumour.

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Section: In Vitro Fluorescence Imagingmentioning

confidence: 99%

Multifunctional Hf/Mn-TCPP Metal-Organic Framework Nanoparticles for Triple-Modality Imaging-Guided PTT/RT Synergistic Cancer Therapy

Bao¹,

Zu²,

Wang³

et al. 2020

IJN

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“…In 2018, Zhang et al fabricated a hepatocyte-targeting uorescent probe 7 for the rst time. 24 In this work, a galactose unit was selected not only as the hepatocyte targeting moiety, but also to improve the water solubility of probe 7. Taking advantage of the meritorious properties, the probe was successfully applied to visualize hepatocellular NO in both HepG2 cells and zebrash.…”

Section: O-phenylenediamine (Opd)-based Reactionsmentioning

confidence: 99%

Small-molecule fluorescent probes for imaging gaseous signaling molecules: current progress and future implications

et al. 2020

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“…There is also increasing interest in developing novel fluorescent chemical probes for various metabolites, ions and redox species. Some of these tools have been successfully tested in zebrafish embryos such as a fluorescent sensor to detect Nitric Oxide in liver cells of zebrafish (Zhang et al, 2018a). Other probes include a polymer micelles-based ratio-metric fluorescent probe for hypochlorous acid (HClO) to monitor HClO generation during liver injury in vivo in zebrafish embryos (Zhang et al, 2018b).…”

Section: Tissue-resolution Of Metabolic Regulation Using Reporter Toolsmentioning

confidence: 99%

Nano-Sampling and Reporter Tools to Study Metabolic Regulation in Zebrafish

Dickmeis

Feng

Mione

et al. 2019

Front. Cell Dev. Biol.

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In the past years, evidence has emerged that hallmarks of human metabolic disorders can be recapitulated in zebrafish using genetic, pharmacological or dietary interventions. An advantage of modeling metabolic diseases in zebrafish compared to other “lower organisms” is the presence of a vertebrate body plan providing the possibility to study the tissue-intrinsic processes preceding the loss of metabolic homeostasis. While the small size of zebrafish is advantageous in many aspects, it also has shortcomings such as the difficulty to obtain sufficient amounts for biochemical analyses in response to metabolic challenges. A workshop at the European Zebrafish Principal Investigator meeting in Trento, Italy, was dedicated to discuss the advantages and disadvantages of zebrafish to study metabolic disorders. This perspective article by the participants highlights strategies to achieve improved tissue-resolution for read-outs using “nano-sampling” approaches for metabolomics as well as live imaging of zebrafish expressing fluorescent reporter tools that inform on cellular or subcellular metabolic processes. We provide several examples, including the use of reporter tools to study the heterogeneity of pancreatic beta-cells within their tissue environment. While limitations exist, we believe that with the advent of new technologies and more labs developing methods that can be applied to minimal amounts of tissue or single cells, zebrafish will further increase their utility to study energy metabolism.

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In vivo imaging of hepatocellular nitric oxide using a hepatocyte-targeting fluorescent sensor

Cited by 104 publications

References 55 publications

<p>Multifunctional Hf/Mn-TCPP Metal-Organic Framework Nanoparticles for Triple-Modality Imaging-Guided PTT/RT Synergistic Cancer Therapy</p>

<p>Multifunctional Hf/Mn-TCPP Metal-Organic Framework Nanoparticles for Triple-Modality Imaging-Guided PTT/RT Synergistic Cancer Therapy</p>

Small-molecule fluorescent probes for imaging gaseous signaling molecules: current progress and future implications

Nano-Sampling and Reporter Tools to Study Metabolic Regulation in Zebrafish

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