In Vivo Immunomodulatory Effect of Tilia x viridis Extracts on Normal Lymphocyte Proliferation: A Direct and an Indirect Action

Davicino, Roberto; Zettler, Gabriela; Brizi, Margarita Rodriguez; Marrassini, Carla; Ferraro, Graciela; Filip, Roxana; Anesini, Claudia

doi:10.1002/ptr.3432

Cited by 6 publications

(2 citation statements)

References 16 publications

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“…Similar results were obtained in a study which demonstrated an in vivo immunomodulatory effect with direct and indirect actions with a flower extract of Tilia sp. to improve the antitumoral activity [ 9 ].…”

Section: Discussionmentioning

confidence: 99%

Antileishmanial activity of a mixture ofTridax procumbensandAllium sativumin mice

et al. 2014

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We tested a mixture of Tridax procumbens, known for its direct action against Leishmania mexicana, and Allium sativum, known for its immunomodulatory effect, as an alternative to treat cutaneous leishmaniasis. Acute oral toxicity was tested with the Up-and-Down Procedure (UDP) using a group of healthy mice administered with either T. procumbens or A. sativum extracts and compared with a control group. Liver injury and other parameters of toxicity were determined in mice at day 14. The in vivo assay was performed with mice infected with L. mexicana promastigotes and treated with either a mixture of T. procumbens and A. sativum or each extract separately. The thickness of the mice’s footpads was measured weekly. After the 12-week period of infection, blood samples were obtained by cardiac puncture to determine the total IgG, IgG1 and IgG2a immunoglobulins by a noncommercial indirect ELISA. We showed that the mixture of T. procumbens and A. sativum extracts was better at controlling L. mexicana infection while not being toxic when tested in the acute oral toxicity assay in mice. An increase in the ratio of IgG2a/IgG1 indicated a tendency to raise a Th1-type immune response in mice treated with the mixture. The mixture of T. procumbens and A. sativum extracts is a promising natural treatment for cutaneous leishmaniasis and its healing effects make it a good candidate for a possible new phytomedicine.

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Section: Discussionmentioning

confidence: 99%

Antileishmanial activity of a mixture ofTridax procumbensandAllium sativumin mice

et al. 2014

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“…By other way, E presented more rutin content (2.7 times more than AE). Previously it was demonstrated that, rutin exerted Px "per se" activity related to H 2 O 2 elimination [3], but also it was demonstrated that an aqueous extract from T. x viridis presents stimulating effect on normal lymphocytes without Con A pre-stimulation, not only "in vitro" but also "in vivo", in relation to rutin presence [34]. So, taking into account the Px "per se" activity of rutin, this compound probably could not be related to the antiproliferative action of the extracts on tumoral cells.…”

Section: Discussionmentioning

confidence: 99%

Comparative Antiproliferative Action of Two Extracts from Tilia x viridis on Normal and Tumoral Lymphocytes: Rela-tionship with Antioxidant Activity

Brizi¹,

Marrassini²,

Zettler³

et al. 2012

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Tilia species have been used in Asia, Europe and in America to treat anxiety and also for the treatment of colds and inflammation. The oxygen reactive species (ROS) (hydrogen peroxide (H2O2) and the superoxide anion (O2) are involve in the balance cell proliferation/death in lymphocytes. It was reported the presence of flavonoids in Tilia species which possess antioxidant properties. The aim of this study was to determine comparatively the effect of an aqueous (AE) and ethanol (E) extract from Tilia x viridis, on the proliferation of tumoral and normal concanavalin A stimulated murine lymphocytes in relation to antioxidant activities such as peroxidase (Px), catalase (CAT) and superoxide dismutase (SOD) activities involves in H2O2 modulation. Also a phytochemical pattern of the two extracts in relation to flavonoids content was determined. Both extracts presented antiproliferative action on both type of lymphocytes but E was more selective on the tumoral lymphocytes inhibition (EC50 (µg/ml, Mean ±SEM) (tumoral): 50 ± 4; EC50; (normal lymphocytes): 323 ± 20); this action was related to a high polyphenols content (150 ± 10 mg/g extract) and high “per se” SOD and low Px activities. In conclusion, the extracts could be a source of antioxidant compounds which contribute to a selective antiproliferative action on tumoral cells, acting through the modulation of H2O2 levels

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Phytopharmacological review on flowers: Source of inspiration for drug discovery

Arya

Kumar

Gautam

2014

Biomedicine & Preventive Nutrition

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In Vivo Immunomodulatory Effect of Tilia x viridis Extracts on Normal Lymphocyte Proliferation: A Direct and an Indirect Action

Cited by 6 publications

References 16 publications

Antileishmanial activity of a mixture ofTridax procumbensandAllium sativumin mice

Antileishmanial activity of a mixture ofTridax procumbensandAllium sativumin mice

Comparative Antiproliferative Action of Two Extracts from <i>Tilia x viridis</i> on Normal and Tumoral Lymphocytes: Rela-tionship with Antioxidant Activity

Phytopharmacological review on flowers: Source of inspiration for drug discovery

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In Vivo Immunomodulatory Effect of Tilia x viridis Extracts on Normal Lymphocyte Proliferation: A Direct and an Indirect Action

Cited by 6 publications

References 16 publications

Antileishmanial activity of a mixture ofTridax procumbensandAllium sativumin mice

Antileishmanial activity of a mixture ofTridax procumbensandAllium sativumin mice

Comparative Antiproliferative Action of Two Extracts from &lt;i&gt;Tilia x viridis&lt;/i&gt; on Normal and Tumoral Lymphocytes: Rela-tionship with Antioxidant Activity

Phytopharmacological review on flowers: Source of inspiration for drug discovery

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Comparative Antiproliferative Action of Two Extracts from <i>Tilia x viridis</i> on Normal and Tumoral Lymphocytes: Rela-tionship with Antioxidant Activity