<i>In Vivo</i>Magnetic Resonance Imaging to Detect Malignant Melanoma in Adult Zebrafish

Kabli, Samira; He, Shuning; Spaink, Herman P.; Hurlstone, Adam; Jagalska, Ewa Snaar; Groot, Huub J. M. de; Alia, A.

doi:10.1089/zeb.2009.0649

Cited by 22 publications

(21 citation statements)

References 22 publications

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“…However, in the embryonic stage of zebrafish, OCT has difficulty in visualizing the weak vascular circulation with sufficient contrast. Besides optical imaging methods, magnetic resonance microimaging (μMRI), micro computed tomography (Micro-CT), and ultrasound biomicroscopy, have been applied in zebrafish studies [16][17][18]. However, μMRI suffers from the expensive cost and difficulty in deriving functional parameters.…”

Section: Introductionmentioning

confidence: 99%

Label-free photoacoustic imaging of the cardio-cerebrovascular development in the embryonic zebrafish

Chen¹,

Jin²,

Qi³

et al. 2017

Biomed. Opt. Express

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Abstract:Zebrafish play an important role in biology, pharmacology, toxicology, and medicine. The cardio-cerebrovascular development of zebrafish is particularly critical to understand both brain disorders and cardiovascular diseases in human. In this paper, we applied optical resolution photoacoustic microscopy (ORPAM) to image the whole-body vasculature of the embryonic zebrafish with a special focus on the development of the cardiocerebrovascular system. Using the intrinsic optical absorption contrast of the embryo, we successfully visualized the formation of the cardio-cerebrovascular network in highresolution using a 10 × objective, and monitored the whole-body vascular development using a 4 × objective. In addition, we evaluated the impact of the eggshell and pigment inhibitor on the image quality. Our results suggest that ORPAM is capable of studying the cardiocerebrovascular development of zebrafish in the embryonic stage, and thus has the potential to investigate the cardiovascular and cerebrovascular diseases of human in the future.

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Section: Introductionmentioning

confidence: 99%

Label-free photoacoustic imaging of the cardio-cerebrovascular development in the embryonic zebrafish

Chen¹,

Jin²,

Qi³

et al. 2017

Biomed. Opt. Express

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“…1 Most recently, various NMR-based techniques for imaging and chemical analysis (including HR-MAS NMR) have been reported for the zebrafish as an increasingly important animal model. [2][3][4][5][6] To date, however, NMR has been largely applied to adult stages of zebrafish, and has enabled in vivo imaging, and localized chemical analyses, in this system. With respect to the former, NMR-based microimaging (lMRI) was recently demonstrated with live zebrafish and specifically applied to the detection and characterization of melanomas.…”

mentioning

confidence: 99%

“…With respect to the former, NMR-based microimaging (lMRI) was recently demonstrated with live zebrafish and specifically applied to the detection and characterization of melanomas. 2,3 Localized in vivo high-resolution spectroscopy, likewise, was achieved in the brain of adult zebrafish (with a spatial resolution of voxels as small as 3.3 lL) and specifically utilized, as an example, for metabolic profiling of spatially defined regions of the brain including, most recently, effective lipid profiling. 4,5 Although the past studies have largely focused on adult stages of the zebrafish, one such study applied NMR, and HR-MAS specifically, for chemical analysis of intact embryonic stages and, in particular, was able to quantify ethanol levels as part of exposure studies.…”

mentioning

confidence: 99%

High-Resolution Magic Angle Spinning Nuclear Magnetic Resonance of Intact Zebrafish Embryos Detects Metabolic Changes Following Exposure to Teratogenic Polymethoxyalkenes from Algae

et al. 2016

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Techniques based on nuclear magnetic resonance (NMR) for imaging and chemical analyses of in vivo, or otherwise intact, biological systems are rapidly emerging and finding diverse applications within a wide range of fields. Very recently, several NMR-based techniques have been developed for the zebrafish as a model animal system. In the current study, the novel application of high-resolution magic angle spinning (HR-MAS) NMR is presented as a means of metabolic profiling of intact zebrafish embryos. Toward investigating the utility of HR-MAS NMR as a toxicological tool, these studies specifically examined metabolic changes of embryos exposed to polymethoxy-1-alkenes (PMAs)-a recently identified family of teratogenic compounds from freshwater algae-as emerging environmental contaminants. One-dimensional and two-dimensional HR-MAS NMR analyses were able to effectively identify and quantify diverse metabolites in early-stage (£36 h postfertilization) embryos. Subsequent comparison of the metabolic profiles between PMA-exposed and control embryos identified several statistically significant metabolic changes associated with subacute exposure to the teratogen, including (1) elevated inositol as a recognized component of signaling pathways involved in embryo development; (2) increases in several metabolites, including inositol, phosphoryl choline, fatty acids, and cholesterol, which are associated with lipid composition of cell membranes; (3) concomitant increase in glucose and decrease in lactate; and (4) decreases in several biochemically related metabolites associated with central nervous system development and function, including c-aminobutyric acid, glycine, glutamate, and glutamine. A potentially unifying model/hypothesis of PMA teratogenicity based on the data is presented. These findings, taken together, demonstrate that HR-MAS NMR is a promising tool for metabolic profiling in the zebrafish embryo, including toxicological applications.

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“…New methods for creating gene modifications or disruptions, such as zinc finger nucleases and transcription activator-like effector nucleases (TALENS), are being applied in zebrafish to generate targeted, heritable genetic mutations. 21,42,70,95 Recent technological advances for analyzing tumor development in zebrafish in vivo, including magnetic resonance imaging, 49 ultrasound biomicroscopy, 34 and high-throughput fluorescent macroscopic imaging, 101 reflect the development and adaptation of increasingly sophisticated systems tailored to zebrafish research.…”

Section: Zebrafish Cancer Models: Future Directionsmentioning

confidence: 99%

Zebrafish Models for Human Cancer

Shive

2012

Vet Pathol

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For decades, the advancement of cancer research has relied on in vivo models for examining key processes in cancer pathogenesis, including neoplastic transformation, progression, and response to therapy. These studies, which have traditionally relied on rodent models, have engendered a vast body of scientific literature. Recently, experimental cancer researchers have embraced many new and alternative model systems, including the zebrafish (Danio rerio). The general benefits of the zebrafish model for laboratory investigation, such as cost, size, fecundity, and generation time, were quickly superseded by the discovery that zebrafish are amenable to a wide range of investigative techniques, many of which are difficult or impossible to perform in mammalian models. These advantages, coupled with the finding that many aspects of carcinogenesis are conserved in zebrafish as compared with humans, have firmly established a unique niche for the zebrafish model in comparative cancer research. This article introduces methods for generating cancer models in zebrafish and reviews a range of models that have been developed for specific cancer types. Keywords animal disease models, cancer, genetic modification, zebrafishThe zebrafish (Danio rerio) emerged as a model for scientific research in the 1930s, with many early studies devoted to the investigation of developmental processes in embryos. 53 The use of zebrafish in research has subsequently expanded into a broad range of disciplines. As a research species, zebrafish offer certain advantages over traditional vertebrate models, including rapid generation time, high fecundity, external embryonic development, a capacity for high stocking density in relatively small areas, and lower maintenance costs. 64 In recent years, the zebrafish has come into its own as a relevant comparative model for a diverse spectrum of human diseases.Although zebrafish are relative newcomers to the field of comparative cancer research, these small animals have emerged as a powerful tool for investigators. As vertebrate organisms, they share many features of embryonic development and adult anatomy that are critical for comparative animal models. 64,71 In the world of cancer research, this has important implications ranging from conserved signaling pathways and transcriptional regulatory mechanisms, to organogenesis and tissue differentiation, to cellular transformation and tumor initiation. Zebrafish cancer models have been produced by a variety of methods, leading to a broad spectrum of cancer susceptibilities in animals of multiple age groups. Flexibility in genetic manipulation, adaptability to current tools for cancer analysis, and capacity to define cellular and molecular interactions in vivo provide the zebrafish with its own very unique set of advantages for the investigation of cancer biology. Mechanisms for Developing Cancer Models in ZebrafishZebrafish cancer models have been developed by a number of mechanisms (Table 1). Although initial studies relied on chemical carcinogens for t...

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In VivoMagnetic Resonance Imaging to Detect Malignant Melanoma in Adult Zebrafish

Cited by 22 publications

References 22 publications

Label-free photoacoustic imaging of the cardio-cerebrovascular development in the embryonic zebrafish

Label-free photoacoustic imaging of the cardio-cerebrovascular development in the embryonic zebrafish

High-Resolution Magic Angle Spinning Nuclear Magnetic Resonance of Intact Zebrafish Embryos Detects Metabolic Changes Following Exposure to Teratogenic Polymethoxyalkenes from Algae

Zebrafish Models for Human Cancer

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