“…37,38 Due to the dynamicity of the lysosomal compartment, imaging lysosomal properties to comprehend its activities is extremely fascinating and to pursue this, numerous synthetic organic lysotrackers have been reported by various research groups. [38][39][40][41] The majority of such reported lysosome targeting probes and commercially available lysotrackers like Neutral Red (NR), DND-189, and DND-99 suffer from pHdependent fluorescence, interference from non-specific binding, background fluorescence, insufficient photostability, and cytotoxicity. 26,42,43 .…”