<i>In Vivo</i> Production of Various Cytokines in Splenocytes of Sheep Erythrocyte‐Immunized Mice after Intravenous Administration of Bacterial Lipid A

Mashimo, Jun-ichi; Mita, Akira

doi:10.1111/j.1348-0421.1995.tb02185.x

Cited by 4 publications

(3 citation statements)

References 22 publications

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“…The results suggest that sensitization protocol up regulates Th1 and Th2 cytokine production significantly as compared to unsensitized control (Tables 1 and 2). Such upregulation effect of SRBC on Th1 and Th2 cytokines is consistent with previous reports (Mashimo and Mita, 1995).…”

Section: Effect Of Sensitization Protocol On Selected Immune Markerssupporting

confidence: 93%

Immunomodulatory activity of Asparagus racemosus on systemic Th1/Th2 immunity: Implications for immunoadjuvant potential

Gautam

Saha

Bani

et al. 2009

Journal of Ethnopharmacology

179

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Section: Effect Of Sensitization Protocol On Selected Immune Markerssupporting

confidence: 93%

Immunomodulatory activity of Asparagus racemosus on systemic Th1/Th2 immunity: Implications for immunoadjuvant potential

Gautam

Saha

Bani

et al. 2009

Journal of Ethnopharmacology

179

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“…We previously reported that orally injected PTCF strongly enhanced the splenic anti-sheep red blood cells (SRBC) plaque-forming cell (PFC) at the doses of 3-30 mg/kg/day [4]. The anti-SRBC PFC response is humoral and cellular immune responses requiring activation of B cells, T cells and monocytes [21], and the enhanced cytokines such as IL-2 [22], IFN-g [23], IL-4, IL-5 and IL-6 [24] correlate with PFC number and IgM titer. In this experiment, PTCF selectively triggered and potentiated IL-2 and IFN-g production in the PP cells (Figs.…”

Section: Discussionmentioning

confidence: 99%

The liquid culture filtrates of Paecilomyces tenuipes (Peck) Samson (=Isaria japonica Yasuda) and Paecilomyces cicadae (Miquel) Samson (=Isaria sinclairii (Berk.) Llond) regulate Th1 and Th2 cytokine response in murine Peyer's patch cells in vitro and ex vivo

Takano

Yahagi

et al. 2005

International Immunopharmacology

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“…This may partly explain the results presented herein that show a single LPS injection induces significant suppression of antibody titers, but does not affect on the number of antibody producing spleen cells. Simultaneous injection of SRBC and lipid A from Salmonella Minnesota R595 enhances the number of IgM-plaque-forming spleen cells in mice 3 and 4 days after injection and the serum IgM titer 4 and 5 days after injection [7]. Thus, the timing of LPS injection may complicate the consequent immune response to particular antigens.…”

Section: Discussionmentioning

confidence: 99%

Effect of Lipopolysaccharide (LPS) Injection on the Immune Responses of LPS-Sensitive Mice

Ogikubo¹,

Norimatsu

Sasaki³

et al. 2004

The Journal of Veterinary Medical Science

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ABSTRACT. The effect of lipopolysaccharide (LPS) on humoral and cell-mediated immunity was assessed using LPS-sensitive C3H/HeN mice. A single injection of LPS significantly decreased the anti-sheep red blood cells (SRBC) antibody titers, but not the number of anti-SRBC antibody producing spleen cells. In contrast, double LPS injection did not significantly decrease the anti-SRBC titers and even increased the number of anti-SRBC antibody producing spleen cells. Similarly, single LPS injection significantly suppressed the swelling of the footpad, but double LPS injection caused milder suppression. These results suggest that a difference in the level and timing of exposure to LPS may influence the immune response to infection or vaccination. KEY WORDS: C3H/HeN, immune response, lipopolysaccharide, tolerance.J. Vet. Med. Sci. 66(10): 1189-1193, 2004 In an effort to improve the health of domestic animals, chronic infection is recently being paid more attention than acute epidemic infection, as most acute infections are under control. Since many chronic infections are caused by gramnegative bacteria, vaccines against these microbes have been increasingly produced, most of which are inactivated whole-cell vaccines. Lipopolysaccharide (LPS), which is a common component of the cell walls of gram-negative bacteria, is capable of eliciting a wide variety of pathophysiological effects such as endotoxin shock, tissue injury, and lethality in both man and animals [8,10]. In addition, LPS, which is known to be a potent stimulant of the host immune system, stimulates proliferation and differentiation of B cells and initiates the activation of mononuclear phagocytes such as macrophages, thus enhancing immune responses [6,9,17,18].It has been reported that LPS can induce apoptosis of lymphocytes and the elevation of serum level of tumor necrosis factor (TNF)-α in both piglets and mice [4,5,13,14]. Moreover, it has been recently hypothesized that Tolllike receptor 4 (TLR4) is the critical receptor for LPS signaling in host defense, and the innate immune system may be involved in the regulation of the adaptive immunity, which is mediated by B and T lymphocytes [16,21]. However, little is known about the effects of LPS on the immune response of domestic animals as well as the mechanism behind.In this report, we describe the effect of single and double LPS injections on the immune responses of an LPS-sensitive mouse strain as a model for domestic animals. MATERIALS AND METHODS LPS:LPS from Escherichia coli O55:B5 extracted by the hot phenol-water extraction method was purchased from Difco laboratories, U.S.A. and was suspended in pyrogenfree saline (Otsuka, Tokyo, Japan).Quantification of antibody titers and antibody producing spleen cells: Eight-week-old female mice of the LPS-sensitive C3H/HeN strain (Japan SLC, Inc., Shizuoka, Japan) were intravenously immunized with 0.2 ml of 5% of sheep red blood cells (SRBC) and boosted 14 days later in an identical manner. The single LPS injection (single LPS) group was intravenously injected...

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In Vivo Production of Various Cytokines in Splenocytes of Sheep Erythrocyte‐Immunized Mice after Intravenous Administration of Bacterial Lipid A

Cited by 4 publications

References 22 publications

Immunomodulatory activity of Asparagus racemosus on systemic Th1/Th2 immunity: Implications for immunoadjuvant potential

Immunomodulatory activity of Asparagus racemosus on systemic Th1/Th2 immunity: Implications for immunoadjuvant potential

The liquid culture filtrates of Paecilomyces tenuipes (Peck) Samson (=Isaria japonica Yasuda) and Paecilomyces cicadae (Miquel) Samson (=Isaria sinclairii (Berk.) Llond) regulate Th1 and Th2 cytokine response in murine Peyer's patch cells in vitro and ex vivo

Effect of Lipopolysaccharide (LPS) Injection on the Immune Responses of LPS-Sensitive Mice

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