The liquid culture filtrates of Paecilomyces tenuipes (Peck) Samson (=Isaria japonica Yasuda) and Paecilomyces cicadae (Miquel) Samson (=Isaria sinclairii (Berk.) Llond) regulate Th1 and Th2 cytokine response in murine Peyer's patch cells in vitro and ex vivo

Takano, Fumihide; Yahagi, Nobuo; Yahagi, Remiko; Takada, Shoji; Yamaguchi, M.; Shoda, Satoko; Murase, Takeshi; Fushiya, Shinji; Ohta, Tomihisa

doi:10.1016/j.intimp.2005.01.005

Cited by 41 publications

(26 citation statements)

References 25 publications

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“…[28][29][30] We previously reported that intestinal lymphoid cells from mice injected orally with culture fluid from entomogenous fungus, Paecilomyces tenuipes, selectively induced Th1 cytokines (IL-2 and IFN-g) production, and the culture fluid has the potential of a therapeutic or preventive agent for allergy. 31) Thus, it is possible to suggest that the mechanism by which PSE and PA decrease the levels of both IgE and total IgG1 is in part due to an induction of Th2-depolarization. Furthermore, it is also shown that oral administration of GP and AP reduced the IL-4 level without any significant affect on IFN-g, which may explain why the suppressive effects of these polyphenols are weaker than those of PSE and PA.…”

Section: Discussionmentioning

confidence: 99%

Aqueous Extract of Peanut Skin and Its Main Constituent Procyanidin A1 Suppress Serum IgE and IgG1 Levels in Mice-Immunized with Ovalbumin

Takano

Takata

Yoshihara

et al. 2007

Biological & Pharmaceutical Bulletin

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Section: Discussionmentioning

confidence: 99%

Aqueous Extract of Peanut Skin and Its Main Constituent Procyanidin A1 Suppress Serum IgE and IgG1 Levels in Mice-Immunized with Ovalbumin

Takano

Takata

Yoshihara

et al. 2007

Biological & Pharmaceutical Bulletin

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“…10,11) In our previous report, liquid culture filtrates of an entomoparasitic fungus, Paecilomyces tenuipes (ϭIsaria japonica YASUDA) (PTCF), selectively stimulated intestinal T cell immune responses, and the active constituent was identified as a glycoprotein-rich fraction. 12) Furthermore, the glycoprotein also enhanced myeloid growth factors, such as GM-CSF, in cultured Peyer's patch cells in response to concanavalin A. 12) In the present study, we investigated whether PTCF and its main ingredient glycoprotein from the PTCF recovers anemia in 5-FU-treated mice.…”

Section: The Liquid Culture Filtrates Of Entomogenous Fungus Paecilommentioning

confidence: 95%

“…12) Furthermore, the glycoprotein also enhanced myeloid growth factors, such as GM-CSF, in cultured Peyer's patch cells in response to concanavalin A. 12) In the present study, we investigated whether PTCF and its main ingredient glycoprotein from the PTCF recovers anemia in 5-FU-treated mice. 5-FU mainly exerts its cytotoxic effect by inhibiting thymidylate synthase activity, and has often been used in chemotherapeutic combinations for malignancy.…”

Section: The Liquid Culture Filtrates Of Entomogenous Fungus Paecilommentioning

confidence: 95%

“…Conidiospores formed from the new conidia were carefully collected, floated on PT medium without agar and further cultured for 200 d under the same conditions. 12,15,16) Isolation of Glycoprotein-Enriched Fraction (PGF) from Culture Filtrates of P. tenuipes (PTCF) Hyphae and conidia of P. tenuipes were carefully and completely removed from the surface of the culture medium by filtration (pore size: 0.45 mm) and the filtrates were centrifuged at 12000ϫg for 60 min. The supernatants were lyophilized to give a darkbrownish powder (PTCF) in a yield of 0.3% (w/v).…”

Section: Methodsmentioning

confidence: 99%

“…The supernatants were lyophilized to give a darkbrownish powder (PTCF) in a yield of 0.3% (w/v). Isolation of PGF from the PTCF was performed by the methods described in our previous report 12) with minor modifications. Briefly, lyophilized PTCF powder was dissolved in distilled water (1 g/30 ml) and dialyzed (MW cut-off: 7000-8000 Da) against a 10-fold volume of distilled water at 4°C for 18 h. The resulting three samples were combined and lyophilized to give a light-brownish powder (460 mg).…”

Section: Methodsmentioning

confidence: 99%

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The Liquid Culture Filtrates of Entomogenous fungus Paecilomyces tenuipes and Its Glycoprotein Constituent Protects against Anemia in Mice Treated with 5-Fluorouracil

Takata

Tanaka

Yahagi

et al. 2008

Biological & Pharmaceutical Bulletin

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Severe anemia that is resistant to medical treatment is often observed in patients with malignancies who are undergoing chemotherapy, and the pathogenesis of this anemia and leukopenia is multifactorial.1) Low levels of circulating hematopoietic growth factors, such as erythropoietin (EPO) and some cytokines, that shorten the survival time of circulating red blood cells (RBCs) and decrease the number of immature erythroid cells in the bone marrow, probably due to chemotherapy or chronic inflammation, have been demonstrated as causes of anemia.2) To cope with chronic anemia, blood transfusions as well as EPO have been used clinically in various situations. Clinical trials of EPO in patients undergoing cancer chemotherapy have demonstrated prevention of anemia development, although complete recovery has not been found.3) Clibon et al. 4) reported that EPO cannot sufficiently overcome the reduced hematopoiesis induced by the inflammatory cytokine tumor necrosis factor (TNF)-a. Myelopoietic growth factors, such as granulocytemacrophage-colony-stimulating factor (GM-CSF), monocyte colony-stimulating factor (M-CSF) and granulocyte-colonystimulating factor (G-CSF), have also been used clinically for leukopenia in cancer chemotherapy.5) In addition to hematopoietic growth factors and transfusion, Japanese herbal medicines, such as Juzen-taiho-to, have been used clinically to ameliorate erythrocytopenia, fatigue or anorexia in patients undergoing cancer chemotherapy.6,7) In one clinical trial, Juzen-taiho-to was found to enhance the peripheral blood counts in cancer patients receiving radiation therapy, 6) and the active constituents were identified as oleic and linoleic acids. 7) Furthermore, an anti-cancer polysaccharide, lentinan, has been shown to stimulate the proliferation of immature erythroid progenitors, burst forming units-erythroid (BFU-E), and to improve 5-fluorouracil (5-FU)-induced reductions in the number of BFU-E in mice. 8) We previously reported that hot-water extracts from Angelica acutiloba KITAGAWA, which is used as a herbal medicine for postmenstrual blood loss and EPO-resistant anemia in chronic renal failure, and its main active polysaccharide constituent increased peripheral RBC and reticulocyte counts, as well as BFU-E mix and colony forming units-erythroid (CFU-E) in cultured bone marrow cells from mice with 5-FU-induced anemia, and that these mechanisms were, in part, due to inhibition of inflammatory cytokine production without EPO mRNA expression in the liver and kidney.9) These results indicate that herbal medicines and plant metabolites that can stimulate the proliferation of erythroid progenitors and have the potential to recover erythrocytopenia in animal models of anemia may be useful for ameliorating anemia in clinical trials.Certain kinds of entomogenous fungi, genera Cordyceps The purpose of the present study was to investigate the efficacy of a liquid culture filtrates of the entomogenous fungus Paecilomyces tenuipes (PTCF) and its main active glycoprotein-enriched (PGF) fraction...

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Anti-hypertensive effect of the dongchunghacho, isaria sinclairii, in the spontaneously hypertensive rats

et al. 2007

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The present study examined the effect of the methanol extract of Isaria sinclairii, a kind of Donchunghacho (Tochukaso), on blood pressure in spontaneously hypertensive rats (SHR). Blood pressure and heart rate were measured after treatment with the methanol extract of I. sinclairii by the indirect tail-cuff method and the direct in vivo model. Starting at 12 weeks of age, male SHR were treated with the extracts for 2 or 4 weeks. We found that, when compared to untreated control SHR, oral treatment with I. sinclairii methanol extract (30 mg/kg/day) remarkably decreased systolic blood pressure from 200 to 112 mmHg and decreased diastolic blood pressure from 114 to 88 mmHg. Furthermore, efficacy of methanol extract of I. sinclairii was superior to captopril (30 mg/kg/mL, positive control), an angiotensin-converting enzyme inhibitor, with a lowering effect that dropped systolic blood pressure from 201 to 130 mmHg and diastolic blood pressure from 102 to 92 mmHg. However, in normal Wistar Kyoto rats, I. sinclairii methanol extract did not significantly change the normal blood pressure, suggesting that this type of Dongchunghacho has a selective effect against hypertension. Therefore, methanol extract of I. sinclairii may be used as an anti-hypertensive food/agent. Furthermore, this extract also has multiple actions such as No production in endothelial cells, inhibiting thrombin-induced blood coagulation by thrombin and mildly decreasing in prostaglandin E2 levels in cultured macrophage cells, all of which might contribute to protection against atherogenesis and thrombus formation. HPLC and MS analysis of methanol extract of I. sinclairii revealed the presence of adenosine.

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The liquid culture filtrates of Paecilomyces tenuipes (Peck) Samson (=Isaria japonica Yasuda) and Paecilomyces cicadae (Miquel) Samson (=Isaria sinclairii (Berk.) Llond) regulate Th1 and Th2 cytokine response in murine Peyer's patch cells in vitro and ex vivo

Cited by 41 publications

References 25 publications

Aqueous Extract of Peanut Skin and Its Main Constituent Procyanidin A1 Suppress Serum IgE and IgG1 Levels in Mice-Immunized with Ovalbumin

Aqueous Extract of Peanut Skin and Its Main Constituent Procyanidin A1 Suppress Serum IgE and IgG1 Levels in Mice-Immunized with Ovalbumin

The Liquid Culture Filtrates of Entomogenous fungus Paecilomyces tenuipes and Its Glycoprotein Constituent Protects against Anemia in Mice Treated with 5-Fluorouracil

Anti-hypertensive effect of the dongchunghacho, isaria sinclairii, in the spontaneously hypertensive rats

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