<i>In vivo</i> reduction in ATP cost of contraction is not related to fatigue level in stimulated rat gastrocnemius muscle

Giannesini, Benoı̂t; Izquierdo, Marguerite; Fur, Yann Le; Cozzone, Patrick J.; Bendahan, David

doi:10.1111/j.1469-7793.2001.00905.x

Cited by 33 publications

(39 citation statements)

References 37 publications

(56 reference statements)

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“…We actually found that ATP production was significantly higher in Mstn Ϫ/Ϫ animals, notably at the end of the stimulation period when glycolytic ATP production was 135% larger compared with Mstn ϩ/ϩ animals. According to the quantitative interpretation of bioenergetics data (3,13,19), this glycolytic flux enhancement was due mainly to the larger changes in pH i during the stimulation period and is in agreement with the acceleration of glucose metabolism through increased glucose uptake and utilization in Mstn Ϫ/Ϫ mouse muscle (24).…”

Section: Nssupporting

confidence: 61%

“…An attractive explanation for this phenomenon would lie in the shift of the fiber-type recruitment pattern since fatigue development in mixed muscle such as gastrocnemius is associated with a decreased recruitment of glycolytic fibers at the expense of oxidative fibers, which are more fatigue resistant (7,13). However, such an issue can be ruled out herein because specific force produced by Mstn Ϫ/Ϫ animals was lower as soon as the onset of the electrical stimulation period, i.e., before fatigue development.…”

Section: Nsmentioning

confidence: 99%

“…ATP production from CK reaction, mitochondrial oxidative phosphorylation, and glycolysis during the stimulation period were calculated according to quantitative interpretation of bioenergetics data (3,13,19). ATP cost of contraction referred to total ATP production scaled to force generation during the same time period.…”

mentioning

confidence: 99%

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Lack of myostatin impairs mechanical performance and ATP cost of contraction in exercising mouse gastrocnemius muscle in vivo

Giannesini

Vilmen

Amthor

et al. 2013

American Journal of Physiology-Endocrinology and Metabolism

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Although it is well established that the lack of myostatin (Mstn) promotes skeletal muscle hypertrophy, the corresponding changes regarding force generation have been studied mainly in vitro and remain conflicting. Furthermore, the metabolic underpinnings of these changes are very poorly documented. To clarify this issue, we have investigated strictly noninvasively in vivo the impact of the lack of Mstn on gastrocnemius muscle function and energetics in Mstn-targeted knockout (Mstn Ϫ/Ϫ ) mice using 1 H-magnetic resonance (MR) imaging and 31 P-MR spectroscopy during maximal repeated isometric contractions induced by transcutaneous electrostimulation. In Mstn Ϫ/Ϫ animals, although body weight, gastrocnemius muscle volume, and absolute force were larger (ϩ38, ϩ118, and ϩ34%, respectively) compared with wild-type (Mstn ϩ/ϩ ) mice, specific force (calculated from MR imaging measurements) was significantly lower (Ϫ36%), and resistance to fatigue was decreased. Besides, Mstn deficiency did not affect phosphorylated compound concentrations and intracellular pH at rest but caused a large increase in ATP cost of contraction (up to ϩ206% compared with Mstn ϩ/ϩ ) throughout the stimulation period. Further, Mstn deficiency limits the shift toward oxidative metabolism during muscle activity despite the fact that oxidative ATP synthesis capacity was not altered. Our data demonstrate in vivo that the absence of Mstn impairs both mechanical performance and energy cost of contraction in hypertrophic muscle. These findings must be kept in mind when considering Mstn as a potential therapeutic target for increasing muscle mass in patients suffering from muscle-wasting disorders. adenosine 5=-triphosphate; skeletal muscle; hypertrophy; energy metabolism; fatigue; nuclear magnetic resonance MYOSTATIN (Mstn) is part of the transforming growth factor-␤ signaling molecule family and acts as an endogenous negative regulator of skeletal muscle growth in mammalians (23). The absence or postnatal blockade of Mstn results in a large and widespread increase in skeletal muscle mass (20,23,24).

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Section: Nssupporting

confidence: 61%

Section: Nsmentioning

confidence: 99%

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Lack of myostatin impairs mechanical performance and ATP cost of contraction in exercising mouse gastrocnemius muscle in vivo

Giannesini

Vilmen

Amthor

et al. 2013

American Journal of Physiology-Endocrinology and Metabolism

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“…Although data from electrically stimulated contractions consistently indicate higher ATP cost as stimulation frequency increases (2,17,20), the impact of voluntary contraction intensity on ME is less clear. Some reports suggest a positive relationship between voluntary contraction intensity and ME (70), while other reports suggest a negative relationship (34), and still others report no change in economy across intensities (3,63).…”

Section: Metabolic Economy During Voluntary Contractionsmentioning

confidence: 97%

Human skeletal muscle metabolic economy in vivo: effects of contraction intensity, age, and mobility impairment

Christie

Tonson

Larsen

et al. 2014

American Journal of Physiology-Regulatory, Integrative and Comp

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Christie AD, Tonson A, Larsen RG, DeBlois JP, Kent JA. Human skeletal muscle metabolic economy in vivo: effects of contraction intensity, age, and mobility impairment. Am J Physiol Regul Integr Comp Physiol 307: R1124 -R1135, 2014. First published August, 27, 2014; doi:10.1152/ajpregu.00083.2014.-We tested the hypothesis that older muscle has greater metabolic economy (ME) in vivo than young, in a manner dependent, in part, on contraction intensity. Twenty young (Y; 24 Ϯ 1 yr, 10 women), 18 older healthy (O; 73 Ϯ 2, 9 women) and 9 older individuals with mild-to-moderate mobility impairment (OI; 74 Ϯ 1, 7 women) received stimulated twitches (2 Hz, 3 min) and performed nonfatiguing voluntary (20, 50, and 100% maximal; 12 s each) isometric dorsiflexion contractions. Torque-time integrals (TTI; Nm·s) were calculated and expressed relative to maximal fat-free muscle cross-sectional area (cm 2 ), and torque variability during voluntary contractions was calculated as the coefficient of variation. Total ATP cost of contraction (mM) was determined from flux through the creatine kinase reaction, nonoxidative glycolysis and oxidative phosphorylation, and used to calculate ME (Nm·s·cm Ϫ2 ·mM ATP Ϫ1 ). While twitch torque relaxation was slower in O and OI compared with Y (P Յ 0.001), twitch TTI, ATP cost, and economy were similar across groups (P Ն 0.15), indicating comparable intrinsic muscle economy during electrically induced isometric contractions in vivo. During voluntary contractions, normalized TTI and total ATP cost did not differ significantly across groups (P Ն 0.20). However, ME was lower in OI than Y or O at 20% and 50% MVC (P Յ 0.02), and torque variability was greater in OI than Y or O at 20% MVC (P Յ 0.05). These results refute the hypothesis of greater muscle ME in old age, and provide support for lower ME in impaired older adults as a potential mechanism or consequence of age-related reductions in functional mobility. bioenergetics; mitochondria; creatine kinase; glycolysis; oxidative phosphorylation WHILE MANY OF THE CHANGES in neuromuscular properties that occur with advanced age, such as declines in strength and contractile velocity (5, 64), can be detrimental to physical function, some age-related physiological changes may act in a compensatory manner and, thereby, help maintain function to some degree. For instance, it has been suggested that a greater proportion of type I muscle fibers (35,55,56), slowed contractile properties (62, 71), and slower motor unit discharge rates, particularly during maximal contractions (8,12,37,38,68), may place older muscle at an economic advantage (41, 49). While age-related differences in muscle metabolic economy (ME; mass-normalized torque produced per unit ATP consumed) have been shown in rat muscle (13, 32), a systematic analysis of muscle ME in aging humans has only recently begun (41,54). Such a focus on muscle ME is critical to informing studies of age-related changes in the energy cost of whole body activities, such as walking, which generally involve measures of...

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“…With these parameters, one can estimate the energy production efficiency of the muscle, such as the speed of PCr degradation and resynthesis, provided that simultaneous force output is recorded (Giannesini et al 2001).…”

Section: P-mrs Measurements (I)mentioning

confidence: 99%

Effects of intrinsic aerobic capacity, aging and voluntary running on skeletal muscle sirtuins and heat shock proteins

Karvinen

Silvennoinen

Vainio

et al. 2016

Experimental Gerontology

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In vivo reduction in ATP cost of contraction is not related to fatigue level in stimulated rat gastrocnemius muscle

Cited by 33 publications

References 37 publications

Lack of myostatin impairs mechanical performance and ATP cost of contraction in exercising mouse gastrocnemius muscle in vivo

Lack of myostatin impairs mechanical performance and ATP cost of contraction in exercising mouse gastrocnemius muscle in vivo

Human skeletal muscle metabolic economy in vivo: effects of contraction intensity, age, and mobility impairment

Effects of intrinsic aerobic capacity, aging and voluntary running on skeletal muscle sirtuins and heat shock proteins

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