2020
DOI: 10.1101/2020.08.31.276279
|View full text |Cite
Preprint
|
Sign up to set email alerts
|

In vivotranscriptome analysis provides insights into host-dependent expression of virulence factors byYersinia entomophagaMH96, during infection ofGalleria mellonella

Abstract: The function of microbes can be inferred from knowledge of genes specifically expressed in natural environments. Here we report the in vivo transcriptome of the entomopathogenic bacterium Yersinia entomophaga MH96, captured during three stages of intrahemocoelic infection in Galleria mellonella. A total of 1,285 genes were significantly upregulated by MH96 during infection; 829 genes responded to in vivo conditions during at least one stage of infection, 289 responded during two stages of infection and 167 tra… Show more

Help me understand this report
View published versions

Search citation statements

Order By: Relevance

Paper Sections

Select...
1
1
1

Citation Types

0
3
0

Year Published

2023
2023
2023
2023

Publication Types

Select...
1

Relationship

0
1

Authors

Journals

citations
Cited by 1 publication
(3 citation statements)
references
References 105 publications
(112 reference statements)
0
3
0
Order By: Relevance
“…Based on the role of RoeA in the transcription of exoproteins including the Yen-Tc and the effect of temperature on virulence regulation in vitro (35) and in vivo in G. mellonella at 25°C and 37°C (15), we investigated the effect of temperature on the translation of RoeA and the Yen-Tc associated Chi1, as an exoprotein proxy.…”
Section: Temperature Dependent Regulation Of Roea and Its Effect On E...mentioning
confidence: 99%
See 2 more Smart Citations
“…Based on the role of RoeA in the transcription of exoproteins including the Yen-Tc and the effect of temperature on virulence regulation in vitro (35) and in vivo in G. mellonella at 25°C and 37°C (15), we investigated the effect of temperature on the translation of RoeA and the Yen-Tc associated Chi1, as an exoprotein proxy.…”
Section: Temperature Dependent Regulation Of Roea and Its Effect On E...mentioning
confidence: 99%
“…Unless stated, cultures were incubated with shaking at 250 rpm in a Ratek model OM11 orbital incubator. Antibiotic concentrations used were (µg mL -1 ): E. coli, kanamycin (50), ampicillin (100); Y. entomophaga, kanamycin (100); spectinomycin (100), chloramphenicol (90), tetracycline (15).…”
Section: Bacterial Strains and Culture Conditionsmentioning
confidence: 99%
See 1 more Smart Citation