<i>In vivo</i> validation of a cell culture test for biocompatibility testing of urinary catheters

Graham, David; Mark, Gerda E.; Pomeroy, A R; Macarthur, E. B.

doi:10.1002/jbm.820180914

Cited by 28 publications

(9 citation statements)

References 9 publications

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“…Animal studies which assessed silicone C-¯ex and hydrogel ureteral stents with smooth surfaces and low coecients of friction, revealed fewer adverse ureteral reactions (ulceration, epithelial hyperplasia, in¯amma-tion, edema) than materials without these surface properties [12,34]. The most common biocompatibility testing procedures used today include animal models [12,13,19,34], cell culture techniques [27,37,41], and in vitro methods used to study the activation of complement, kallikrein-kinin, ®brinolysis and coagulation [61].…”

Section: Urological Biomaterials and Biocompatibilitymentioning

confidence: 99%

Advances in ureteral stent technology

Denstedt¹,

Reid

Sofer³

2000

World J Urol

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This review focuses on technological advances and relevant research related to ureteral stents. The importance of physical and chemical biomaterial type, biocompatibility, material coatings such as hydrogels, and infection related to indwelling ureteral stents are discussed. Recent in vitro and in vivo research has focused on materials that will reduce encrustation and bacterial biofilm formation. The adsorption of antimicrobials onto devices holds promise of reducing infection rates, but multidrug resistant bacteria, short leaching times and adverse side effects make it essential that alternative strategies be investigated. Just so, encrustation limits the long-term use of urinary materials, and a better understanding of factors involved in encrustation are needed to reduce the problem.

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Section: Urological Biomaterials and Biocompatibilitymentioning

confidence: 99%

Advances in ureteral stent technology

Denstedt¹,

Reid

Sofer³

2000

World J Urol

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“…In this study, the cytotoxicity of latex gloves to cultured L-929 cells was determined under various extraction conditions by using the extract dilution method. Since 1980, the cytotoxicity of latex urinary catheters has been widely reported on in clinical and experimental situations using various cell lines, such as, human urothelial cells, V79 cells and L-929 cells 20,21. Therefore, latex gloves are suitable test samples for evaluating the optimal extraction conditions for cytotoxicity tests.…”

mentioning

confidence: 99%

Evaluation of the Extraction Method for the Cytotoxicity Testing of Latex Gloves

et al. 2005

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In this study, the cytotoxicity of medical latex gloves to cultured L-929 cells was determined using various extraction conditions. According to the extraction time and temperature, three types of extraction conditions were used: 1) 24 h at 37℃; 2) 72 h at 37℃; 3) 72 h at 50℃. Also, four different extraction vehicles were used, namely, distilled water (DW), 9 g/l sodium chloride (saline) in DW, and culture media with or without serum. Under the above-mentioned conditions, the samples were extracted and then 2-fold serially diluted in the concentration range 3.13 - 50%. When extracted with either DW or saline for 24 h or 72 h at 37℃, only 50% diluted samples showed distinct cytotoxicity to L-929 cells. Moreover, no cytotoxic potentials were observed when gloves were extracted with DW or saline at 50℃ for 72 h. Cytotoxicity was markedly greater when gloves were extracted with culture medium, irrespective of the presence of serum in the medium. These results suggest that optimal extraction conditions should be established for the cytotoxicity evaluations of biomaterials and medical devices.

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“…Since 1980 the cytotoxicity of latex urinary catheters has been widely reported in clinical and experimental situations using permanent cell lines [2, 3, 4, 5, 6]. …”

Section: Introductionmentioning

confidence: 99%