In Vivo Wound-Healing Effect of Chemical and Green Synthesized Chitosan Nanoparticles Using Lawsonia inermis Ethanolic Extract

Abstract

Wounds can be a result of surgery, an accident, or other factors. There is still a challenge to find effective topical wound-healing agents. This study aims to investigate the wound-healing activity of chemical and green synthesized chitosan nanoparticles (Ch-NPs) using Lawsonia inermis leaves extract. The nanoparticles were morphologically and chemically characterized using X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), and high-resolution transmission electron microscopy (HRTEM). Forty-five adult female albino rats were randomly divided into three groups. The cutaneous surgical wounds were topically treated with 0.9% normal saline (control group), green Ch-NPs (second group), and chemical Ch-NPs gels (third group), respectively. The clinical picture of wounds and histopathological changes were assessed on the 3rd, 7th, 14th, and 21st days post-treatment. X-ray diffraction analysis revealed great crystallinity and purity of nanoparticles. The studied nanoparticles increased the wound contraction percent (WC%), reduced healing time and wound surface area (WSA), and these results were backed up by histological findings that indicated improved epithelialization, dermal differentiation, collagen deposition, and angiogenesis in treated rats compared with control rats (p < 0.05). We concluded that the wound-healing effects of the studied nanoparticles are encouraging, and further studies for complete assessment are still needed.

Back to nature: henna extracts from nanotech to environmental biotechnology – a review

“…Metwally et al ( 2022 ) reported that the green synthesis of ZnO-NPs via the methanolic extract of HLE exhibited outstanding wound-healing efficacy with an undetectable scar, compared to chemically manufactured ZnO-NPs. Additionally, it has been shown that green-synthesized chitosan nanoparticles (Ch-NPs) utilizing HLE increase wound surface area, healing duration, and wound contraction percentage (Metwally et al, 2023 ). Henna seed extract (HSE) and ultrasound vibrations were utilized to create a spherical and irregular mixture of silver and gold nanoparticles (Au–Ag BNPs), with particle sizes ranging from 15 to 35 nm (Akilandaeaswari and Muthu, 2021 ).…”