This study was aimed to evaluate the wound healing activity of both green and chemical Zinc oxide Nanoparticles in equine. Ten animals (nine donkeys and one horse) suffered from traumatized infected or non-infected wounds were used in this study. The visible signs of wound infection were evaluated in six cases, while the other four cases were admitted with fresh recent wounds. Animals were topically treated once a day in and day out and monitored for three weeks. It was observed that, the macroscopic wound contraction percents in animals treated with green Zinc oxide Nanoparticles gel in 1st, 2nd and 3rd weeks were 45%, 76%, and 93.6% respectively. While, those which treated with chemical Zinc oxide Nanoparticles gel were 40.4%, 67.2%, and 90.6% respectively. The study revealed that, green Zinc oxide Nanoparticles gel accelerated the wound healing and cleared the wound infection faster when compared to chemical Zinc oxide Nanoparticles gel. It was concluded that, topical green Zinc oxide Nanoparticles gel can significantly accelerate the process of wound healing and clear the wound infections in wounded equine from clinical field cases.

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Back ground: Synthetic bone substitutes have been advanced, and researches are developed to more effective biomaterials with promising ability to reconstruct lost bone structure. Tricalcium phosphate biomaterials and white Portland cement are among them. The aim of the current study is to assess the effect of β-tricalcium phosphate versus white Portland cement in surgically mandibular bone defect dogs. Material and methods:Six adult dogs were used. The anaesthesia was conducted by iv injection of thiopental sodium 20mg/kg.b.wt. and maintained using (atropine 0.04 mg/kg b.wt., xylazine 1mg/kg.b.wt. and diazepam 1mg/kg.b.wt.in saline iv drop by drop infusion ), three critical size defects were designed at the buccal side of the mandible. The defects were 5 cm apart from each other. One defects filled with sterile white Portland cement, the middle defect filled with β-tricalcium phosphate and the later one was left empty. The dogs were euthanized at the end of 1 and 8 week postoperatively respectively. The specimens were prepared for light microscope using H&E and Masson Tri-chrome staining. Results: The experimental sides of all periods revealed increasing the amount of new bone trabeculae extended from original bone towards the center of the defect with both white Portland cement & β-tricalcium phosphate. While, the control sides displayed few new bone trabeculae at central area and the lateral wall of the bony defects. The amount of new bone trabeculae confirmed the histological results. Conclusion: White Portland cement is a biocompatible osteoconductive economically affordable graft material and it id more efficient than β-tricalcium phosphate. (3476) Altaib Abdel Razik Mohammed, et al.

Wound healing is one of the utmost medical issues in human and veterinary medicine, which explains the urgent need for developing new agents that possess wound-healing activities. The present study aimed to assess the effectiveness of green and chemical ZnO-NPs for wound healing. ZnO-NPs (chemical and green using Lawsonia inermis leaf extract) were synthesized and characterized by XRD; FTIR, and HRTEM. The gels containing the nanomaterials were prepared and inspected. Forty-ve albino rats were divided into three groups, the control group was treated with normal saline 0.9 % and the other two groups were treated with gels containing green and chemical ZnO-NPs, respectively. On the 3rd, 7th, 14th, and 21st dpt, the wounds were clinical and histologically examined. Both nanomaterials have good crystallinity and high purity, but green ZnO-NPs have a longer nanowire length and diameter than chemical ZnO-NPs. The formed gels were highly viscous with a pH of 6.5 to 7. Both prepared gels showed clinical improvements, such as a decrease in WSA and WSA%, increase in WC%, and reduced healing time (P < 0.05) in both treated groups when compared with control group. The histological scoring of this study showed that the epithelization score was signi cantly higher at 21st dpt in treated groups than in the control group (P < 0.05), but the vasculature, necrosis, connective tissue formation, and collagen synthesis scores were mostly similar. The green and chemical ZnO-NPs gels showed promising wound healing properties however, the LI mediated-ZnO-NPs were more effective.

Wounds can be a result of surgery, an accident, or other factors. There is still a challenge to find effective topical wound-healing agents. This study aims to investigate the wound-healing activity of chemical and green synthesized chitosan nanoparticles (Ch-NPs) using Lawsonia inermis leaves extract. The nanoparticles were morphologically and chemically characterized using X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), and high-resolution transmission electron microscopy (HRTEM). Forty-five adult female albino rats were randomly divided into three groups. The cutaneous surgical wounds were topically treated with 0.9% normal saline (control group), green Ch-NPs (second group), and chemical Ch-NPs gels (third group), respectively. The clinical picture of wounds and histopathological changes were assessed on the 3rd, 7th, 14th, and 21st days post-treatment. X-ray diffraction analysis revealed great crystallinity and purity of nanoparticles. The studied nanoparticles increased the wound contraction percent (WC%), reduced healing time and wound surface area (WSA), and these results were backed up by histological findings that indicated improved epithelialization, dermal differentiation, collagen deposition, and angiogenesis in treated rats compared with control rats (p &lt; 0.05). We concluded that the wound-healing effects of the studied nanoparticles are encouraging, and further studies for complete assessment are still needed.