Klebsiellasp. strain C2A isolated from olive oil mill waste is able to tolerate and degrade tannic acid in very high concentrations

Abstract

Four bacterial strains capable of growing in the presence of tannic acid as sole carbon and energy source were isolated from olive mill waste mixtures. 16S rRNA gene sequencing assigned them to the genus Klebsiella. The most efficient strain, Klebsiella sp. strain C2A, was able to degrade 3.5 g L(-1) tannic acid within 35 h with synthesizing gallic acid as main product. The capability of Klebsiella sp. strain C2A to produce tannase was evidenced at high concentrations of tannic acid up to 50 g L(-1) . The bacteria adapted to the toxicity of tannic acids by an increase in the membrane lipid fatty acids degree of saturation, especially in the presence of concentrations higher than 20 g L(-1) . The highly tolerant and adaptable bacterial strain characterized in this study could be used in bioremediation processes of wastes rich in polyphenols such as those derived from olive mills, winery or tanneries.

Nutritive value of treatedQuercus infectoriaandQuercus libanileaves with the tannin‐degrading bacteriumKlebsiella pneumoniaefor ruminant feedingin vitro

“…Therefore, this study was planned to investigate the chemical composition, in vitro gas production (IVGP) and fermentation parameters of Q. infectoria and Q. libani oak leaf following treatment with Klebsiella pneumoniae , a native tannase producing bacterium. The K. pneumoniae used in the present study was isolated from farm soil and this is consistent with other studies that isolated several bacterial strains with phenolic degrading potential from different sources (Arcuri et al ; Pepi et al ; Mosleh et al ).…”

Degradation of euptox A by tannase-producing rumen bacteria from migratory goats

“…), soil, water, industrial wastes (Pepi et al . ), nasal and GI tract of humans and animals. This study affirms occurrence of Klebsiella sp.…”

“…Klebsiella species with multiple metabolic activities are widely distributed in nature including plants (Mart ınez-Romero et al 2015), soil, water, industrial wastes (Pepi et al 2013), nasal and GI tract of humans and animals. This study affirms occurrence of Klebsiella sp.…”

Isolation, Identification and Molecular Characterization of Tannase Producing Klebsiella sp., from the Rumen of Migratory Goats and Sheep

“…All these reports suggest tannase enzyme activity from anaerobic microbes. Tannase activity was reported in Klebsiella species from different sources (Deschamps et al, 1983;Jadhav et al, 2011;Sivashanmugam and Jayaraman, 2011;Pepi et al, 2013) but not from the rumen microbial culture. Information is scarce on molecular characterization of tannase of the gut microbial origin.…”

“…Recently discovered Klebsiella sp. C2A strain proposed by Pepi et al (2013) has not taken into consideration as in NCBI it has partial 16S ribosomal RNA of ~700 nucleotides length only. Sivashanmugam and Jayaraman (2011) reported about Klebsiella pneumoniae MTCC 7162, isolated from tannery effluent.…”