Lactiplantibacillus plantarum 22A-3-induced TGF-β1 secretion from intestinal epithelial cells stimulated CD103+ DC and Foxp3+ Treg differentiation and amelioration of colitis in mice

Lamubol, Jarukan; Ohto, Nobuaki; Kuwahara, Hiroshige; Mizuno, Masashi

doi:10.1039/d1fo00990g

Cited by 6 publications

(19 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In addition, administration of LP22A3 resulted in increased concentrations of IL-10 in the circulatory system, which is recognized as a significant antiinflammatory mediator. 65 The mRNA expression levels of genes related to TGF-β, IL-10, and Foxp3 (a marker indicative of Tregs) exhibited a particular increase inside the small intestines of mice treated with LP22A3. This finding indicates that LP22A3 has a specific impact on the immune response of the small intestine.…”

Section: Effects Of Probiotics On Dcs In Ucmentioning

confidence: 96%

“…This finding indicates that LP22A3 has a specific impact on the immune response of the small intestine. 65 Additionally, the investigation revealed that the administration of LP22A3 resulted in an augmentation of the mRNA expression of aldehyde dehydrogenase 1 family member A2 (Aldh1a2) and a spike in the number of CD103+DCs inside the small intestine. 65 DCs expressing the CD103 marker are of significant importance in the control of the immune system.…”

Section: Effects Of Probiotics On Dcs In Ucmentioning

confidence: 99%

“…The primary objective of the research is to gain insights into the fundamental molecular pathways that contribute to the observed effects of this probiotic strain. The primary emphasis of their investigation lies in assessing the impacts of DSS‐induced colitis on a mouse model 65 . The TLR2 signaling pathway is often linked to an NF‐κB‐dependent pro‐inflammatory reaction.…”

Section: Effects Of Probiotics On Dcs In Ucmentioning

confidence: 99%

“…Recent studies have provided insights into the capacity of probiotics to regulate DCs, presenting a viable approach for treating UC. [64][65][66] Okada and colleagues investigated the potential of a newly discovered probiotic yeast strain, Zygosaccharomyces sapae strain I-6, obtained from Miso, a traditional fermented cuisine in Japan. 64 The researchers examine the influence of this yeast on distinct immune cells inside the DCs and evaluate its capacity to alleviate inflammation in a murine model of colitis produced by DSS.…”

Section: Effects Of Probiotics On Dcs In Ucmentioning

confidence: 99%

See 3 more Smart Citations

Mechanistic insights into the role of probiotics in modulating immune cells in ulcerative colitis

Guo,

2023

Immunity Inflam & Disease

View full text Add to dashboard Cite

BackgroundUlcerative colitis (UC) is a persistent inflammatory disorder that affects the gastrointestinal tract, mainly the colon, which is defined by inflammatory responses and the formation of ulcers. Probiotics have been shown to directly impact various immune cells, including dendritic cells (DCs), macrophages, natural killer (NK) cells, and T and B cells. By interacting with cell surface receptors, they regulate immune cell activity, produce metabolites that influence immune responses, and control the release of cytokines and chemokines.MethodsThis article is a comprehensive review wherein we conducted an exhaustive search across published literature, utilizing reputable databases like PubMed and Web of Science. Our focus centered on pertinent keywords, such as “UC,” ‘DSS,” “TNBS,” “immune cells,” and “inflammatory cytokines,” to compile the most current insights regarding the therapeutic potential of probiotics in managing UC.ResultsThis overview aims to provide readers with a comprehensive understanding of the effects of probiotics on immune cells in relation to UC. Probiotics have a crucial role in promoting the proliferation of regulatory T cells (Tregs), which are necessary for preserving immunological homeostasis and regulating inflammatory responses. They also decrease the activation of pro‐inflammatory cells like T helper 1 (Th1) and Th17 cells, contributing to UC development. Thus, probiotics significantly impact both direct and indirect pathways of immune cell regulation in UC, promoting Treg differentiation, inhibiting pro‐inflammatory cell activation, and regulating cytokine and chemokine release.ConclusionProbiotics demonstrate significant potential in modulating the immune reactions in UC. Their capacity to modulate different immune cells and inflammation‐related processes makes them a promising therapeutic approach for managing UC. However, further studies are warranted to optimize their use and fully elucidate the molecular mechanisms underlying their beneficial effects in UC treatment.

show abstract

Section: Effects Of Probiotics On Dcs In Ucmentioning

confidence: 96%

Section: Effects Of Probiotics On Dcs In Ucmentioning

confidence: 99%

Section: Effects Of Probiotics On Dcs In Ucmentioning

confidence: 99%

Section: Effects Of Probiotics On Dcs In Ucmentioning

confidence: 99%

See 2 more Smart Citations

Mechanistic insights into the role of probiotics in modulating immune cells in ulcerative colitis

Guo,

2023

Immunity Inflam & Disease

View full text Add to dashboard Cite

show abstract

“…Endogenous EGF signaling in crypt–villus axis cells in the intestinal tract promotes the colonization of ISCs and short-lived TA cells. The mechanism of EGF action is triggered after the specific binding to EGFR on the membrane of the cell ( Lamubol et al, 2021 ). EGFR activates other kinases via phosphorylation, initiating the activation of a series of downstream signaling pathways (such as PI3K/AKT and RAS/RAF/MEK/ERK) that ultimately activate intracellular transcription factors and regulate downstream gene transcription to modulate the proliferation and differentiation of cells.…”

Section: Introductionmentioning

confidence: 99%

Molecular regulation after mucosal injury and regeneration in ulcerative colitis

Zheng

Duan

Wen

et al. 2022

Front. Mol. Biosci.

View full text Add to dashboard Cite

Ulcerative colitis (UC) is a chronic nonspecific inflammatory disease with a complex etiology. Intestinal mucosal injury is an important pathological change in individuals with UC. Leucine-rich repeat-containing G protein-coupled receptor 5 (LGR5+) intestinal stem cells (ISCs) exhibit self-renewal and high differentiation potential and play important roles in the repair of intestinal mucosal injury. Moreover, LGR5+ ISCs are intricately regulated by both the Wnt/β-catenin and Notch signaling pathways, which jointly maintain the function of LGR5+ ISCs. Combination therapy targeting multiple signaling pathways and transplantation of LGR5+ ISCs may lead to the development of new clinical therapies for UC.

show abstract

Contamination of bacterial extracellular vesicles (bEVs) in human urinary extracellular vesicles (uEVs) samples and their effects on uEVs study

Noonin

Peerapen

Thongboonkerd

2022

J of Extracellular Bio

View full text Add to dashboard Cite

Bacterial overgrowth is common for improperly stored urine. However, its effects on human urinary extracellular vesicles (uEVs) study had not been previously examined nor documented. This study investigated the presence of bacterial EVs (bEVs) contaminated in uEVs samples and their effects on uEVs study. Nanoscale uEVs were isolated from normal human urine immediately after collection (0-h) or after 25 • Cstorage with/without preservative (10 mM NaN 3 ) for up to 24-h. Turbidity, bacterial count and total uEVs proteins abnormally increased in the 8-h and 24-h-stored urine without NaN 3 . NanoLC-ESI-LTQ-Orbitrap MS/MS identified 6-13 bacterial proteins in these contaminated uEVs samples. PCR also detected bacterial DNAs in these contaminated uEVs samples. Besides, uEVs derived from 8-h and 24-h urine without NaN 3 induced macrophage activation (CD11b and phagocytosis) and secretion of cytokines (IFN-α, IL-8, and TGF-β) from macrophages and renal cells (HEK-293, HK-2, and MDCK). All of these effects induced by bacterial contamination were partially/completely prevented by NaN 3 . Interestingly, macrophage activation and cytokine secretion were also induced by bEVs purified from Escherichia coli. This study clearly shows evidence of bEVs contamination and their effects on human uEVs study when the urine samples were inappropriately stored, whereas NaN 3 can partially/completely prevent such effects from the contaminated bEVs.

show abstract

Lactiplantibacillus plantarum 22A-3-induced TGF-β1 secretion from intestinal epithelial cells stimulated CD103⁺ DC and Foxp3⁺ Treg differentiation and amelioration of colitis in mice

Abstract: In the present study, we evaluated the anti-inflammatory property of Lactiplantibacillus plantarum 22A-3 (LP22A3) and attempted to elucidate the underlying molecular mechanism. The oral administration of LP22A3 significantly inhibited body...

Cited by 6 publications

References 32 publications

Mechanistic insights into the role of probiotics in modulating immune cells in ulcerative colitis

Mechanistic insights into the role of probiotics in modulating immune cells in ulcerative colitis

Molecular regulation after mucosal injury and regeneration in ulcerative colitis

Contamination of bacterial extracellular vesicles (bEVs) in human urinary extracellular vesicles (uEVs) samples and their effects on uEVs study

Contact Info

Product

Resources

About