<i>Lactobacillus</i> spp. reduces ethanol‑induced liver oxidative stress and inflammation in a mouse model of alcoholic steatohepatitis

Hsieh, Ping-Cheng; Chen, Ching‐Wei; Kuo, Yi‐Wei; Ho, Hao-Chung

doi:10.3892/etm.2021.9619

Cited by 26 publications

(20 citation statements)

References 32 publications

(20 reference statements)

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“…The maintenance of liver function forms a positive cycle on stabilizing fat metabolism and reducing fat accumulation [ 42 ]. Some strains of Lactobacillus have been shown to reduce TG content in the liver and alleviate inflammation of alcoholic steatohepatitis in a mouse model [ 44 ]. In this study, probiotic strains AP-32, bv-77, and CP-9 reduced serum AST and ALT levels, indicating a relief on fat metabolism and the restoration of liver function.…”

Section: Discussionmentioning

confidence: 99%

A Combined Supplement of Probiotic Strains AP-32, bv-77, and CP-9 Increased Akkermansia mucinphila and Reduced Non-Esterified Fatty Acids and Energy Metabolism in HFD-Induced Obese Rats

Liao

Huang

et al. 2022

Nutrients

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Obesity is referred to as a condition in which excess body fat has accumulated to an extent that it causes negative impacts on health. The formation of body fat is regulated by complicated networks in relation to energy metabolism, and gut microbiota have been regarded as a key player. Studies have shown that supplements of probiotics provide benefits to health, including an improvement in metabolic syndrome and the control of body weight. In the present study, three probiotic strains, AP-32, bv-77, and CP-9, stood out from nine candidates using a lipid consumption assay, and were subsequently introduced to further animal tests. A rodent model of obesity was induced by a high-fat diet (HFD) in Sprague-Dawley (SD) rats, and three probiotic strains were administered either separately or in a mixture. A low dose (5 × 109 CFU/kg/day) and a high dose (2.5 × 1010 CFU/kg/day) of probiotics were orally provided to obese rats. The bioeffects of the probiotic supplements were evaluated based on five aspects: (1) the body weight and growth rate; (2) ketone bodies, non-esterified fatty acids (NEFAs), and feed efficiency; (3) blood biochemistry; (4) fat content; and (5) gut microbiota composition. Our results demonstrated that the supplement of AP-32, CP-9, and bv-77 alleviated the increasing rate of body weight and prevented the elevation of NEFAs and ketone bodies in obese rats. Although the effect on fat content showed a minor improvement, the supplement of probiotics displayed significant improvements in HFD-induced poor blood biochemical characteristics, such as alanine aminotransferase (ALT), aspartate Transaminase (AST), and uric acid, within 4 weeks. Furthermore, the combined supplement of three strains significantly increased Akkermansia mucinphila as compared with three individual strains, while its enrichment was negatively correlated with NEFAs and energy metabolism. In general, a mixture of three probiotic strains delivered a better outcome than a single strain, and the high dose of supplements provided a more profound benefit than the low dose. In conclusion, three probiotic strains, AP-32, bv-77, and CP-9, can alleviate body fat formation in obese rats. Furthermore, a combined supplement of these three probiotic strains may have potential in treating or controlling metabolic disorders.

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Section: Discussionmentioning

confidence: 99%

A Combined Supplement of Probiotic Strains AP-32, bv-77, and CP-9 Increased Akkermansia mucinphila and Reduced Non-Esterified Fatty Acids and Energy Metabolism in HFD-Induced Obese Rats

Liao

Huang

et al. 2022

Nutrients

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“…Moreover, antioxidants can protect against the oxidative stress induced by alcohol, accompanied with anti-inflammation, antiapoptosis, and autophagy regulation effects, such as milk thistle 31) , betaine 32) , β-carotene 33,34) . In addition, some nutrients can prevent ALD by improving the intestinal permeability and microbiota composition in rats fed with alcohol, such as glutamine 35) , epidermal growth factor (EGF) 36) , probiotics 37) or synbiotics 38) . Although a multitude of animal studies have been demonstrated, clinical trials in human subjects are necessary.…”

Section: Nutrition-related Alternative Medicinementioning

confidence: 99%

A New Perspective on Fish Oil: The Prevention of Alcoholic Liver Disease

Yang

2021

J. Oleo Sci.

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“…IL-10, MIP-3α, IL-1α and IL-12p70 were determined with different levels between Non-Severe and Severe cohorts, suggesting the changes in cytokines were associated with the effectiveness of LI10 against liver damage. The alterations of cytokines were also reported in potential probiotics-treated cohorts with different liver diseases (Hsieh et al, 2021;Karamese et al, 2020;Wang et al, 2019).…”

Section: Discussionmentioning

confidence: 76%

Characteristics of the gut microbiota in Bifidobacterium catenulatum LI10 pretreated rats with lower levels of D-galactosamine-induced liver damage

Zha

Wang

et al. 2022

Journal of Applied Microbiology

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Aims Liver damage has caused great illness in human beings. Bifidobacterium catenulatum LI10 has been determined with protective effect against D‐galactosamine‐induced liver damage. However, due to the sample limitation, the individual difference in its protective effect was not determined. The current study was designed to characterize the gut microbiota of LI10‐pretreated rats with lower levels of liver damage. Methods and Results A series of experiments and bioinformatic analyses were carried out. Two rat cohorts with different levels of liver damage were determined, that is, Non‐Severe and Severe cohorts. Six out of the seven measured liver function variables were lower in the Non‐Severe cohort, while four cytokine variables also yielded differences between the two cohorts. The Non‐Severe and Severe cohorts were determined with distinct gut microbiota, among which ASV14_Parabacteroides and ASV7_Bacteroides were most associated with Non‐Severe and Severe cohorts, respectively. Five phylotypes were determined as structural gatekeepers in the microbiota network of Non‐Severe cohort, ASV135_Lachnospiraceae_NK4A136 of which contributed most to the stability of the network. Conclusions The relevant findings suggest that some gut bacteria could benefit the protective effect of LI10 on lowering the severity of rat liver damage. Significance and Impact of the Study The bacteria benefiting the protective effects of potential probiotics could be further investigated for future clinical application.

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Lactobacillus spp. reduces ethanol‑induced liver oxidative stress and inflammation in a mouse model of alcoholic steatohepatitis

Cited by 26 publications

References 32 publications

A Combined Supplement of Probiotic Strains AP-32, bv-77, and CP-9 Increased Akkermansia mucinphila and Reduced Non-Esterified Fatty Acids and Energy Metabolism in HFD-Induced Obese Rats

A Combined Supplement of Probiotic Strains AP-32, bv-77, and CP-9 Increased Akkermansia mucinphila and Reduced Non-Esterified Fatty Acids and Energy Metabolism in HFD-Induced Obese Rats

A New Perspective on Fish Oil: The Prevention of Alcoholic Liver Disease

Characteristics of the gut microbiota in Bifidobacterium catenulatum LI10 pretreated rats with lower levels of D-galactosamine-induced liver damage

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