Lactobacillus salivarius Ren prevent the early colorectal carcinogenesis in 1, 2-dimethylhydrazine-induced rat model

Abstract

Aims: The objective of this study was to investigate the impact of Lactobacillus salivarius Ren (LS) on modulating colonic micro flora structure and influencing host colonic health in a rat model with colorectal precancerous lesions. Methods and Results: Male F344 rats were injected with 1, 2-dimethylhydrazine (DMH) and treated with LS of two doses (5 9 10 8 and 1 9 10 10 CFU kg À1 body weight) for 15 weeks. The colonic microflora profiles, luminal metabolites, epithelial proliferation and precancerous lesions [aberrant crypt foci (ACF)] were determined. A distinct segregation of colonic microflora structures was observed in LS-treated group. The abundance of one Prevotella-related strain was increased, and the abundance of one Bacillusrelated strain was decreased by LS treatment. These changes were accompanied by increased short-chain fatty acid levels and decreased azoreductase activity. LS treatment also reduced the number of ACF by c. 40% and suppressed epithelial proliferation. Conclusions: Lactobacillus salivarius Ren improved the colonic microflora structures and the luminal metabolisms in addition preventing the early colorectal carcinogenesis in DMH-induced rat model. Significance and Impact of the Study: Colonic microflora is an important factor in colorectal carcinogenesis. Modulating the structural shifts of microflora may provide a novel option for preventing colorectal carcinogenesis. This study suggested a potential probiotic-based approach to modulate the intestinal microflora in the prevention of colorectal carcinogenesis.

Effects of Lactobacillus salivarius Ren on cancer prevention and intestinal microbiota in 1, 2-dimethylhydrazine-induced rat model

“…ACF was detected in DMH-treated animals after 16 weeks in our previous study (Zhu et al, 2014). Some strains such as Bacteroides sp.…”

“…In our previous study, we demonstrated that Ren could modulate colonic microflora and therefore prevent early colorectal carcinogenesis in a DMH-induced rat model (Zhu et al, 2014). However, it was unclear if such an effect is apparent in CRC development.…”

“…We also found that Ren may significantly improve the colonic microflora structures and therefore prevent early colorectal carcinogenesis, as measured by the presence of aber- rant crypt foci (ACF) in a 1,2-dimethyl hydrazine (DMH)-induced rat model (Zhu et al, 2014). Whether such an effect operates in colorectal tumor development remains to be shown.…”

Podem as bactérias ácido lácticas probióticas apresentarem efeito antitumoral em modelo animal de câncer de cólon? Uma revisão da literatura

“…A maioria dos animais teve o câncer de cólon induzido por DMH (Brasil, 2012, Kumar et al 2012, Zhu et al 2014 Urbanska et al (2009) verificaram que para os camundongos tratados L. acidophilus, o peso corporal manteve-se constante durante 17 dias em relação ao grupo de animais controle, minimizando a inflamação intestinal e retardando a progressão de adenomas. Kumar et al (2012) observaram que uso de L. plantarum na alimentação dos animais tratados resultou em um aumento significativo no ganho de peso em comparação ao grupo não tratado.…”

“…Os ACF induzidos por DMH são estabelecidos como um substituto biomarcador de câncer de cólon, considerando sua alta correlação com a carcinogênese e sua recapitulação de características patológicas e moleculares de pacientes potenciais com este câncer (Zhu et al 2014). O câncer de cólon induzido por DMH experimental em ratos é um processo prolongado de múltiplos estágios, apresentando características de cinética celular, molecular e histopatoló-gica da tumorigênese que imita o câncer colorretal humano (Kumar et al 2012).…”