<i>Lactococcus garvieae</i>, a cause of disease in grey mullet, <i>Mugil cephalus</i> L., in Taiwan

Chen, Shuo; Liaw, L-L; Su, Huafang; Ko, S-C; Wu, Chen-Hsuan; Chaung, H-C; Tsai, Y-H; Yang, K-L; Yc, Chen; Chen, Tzung‐Her; Lin, G. G.; Cheng, S-Y; Lin, Y-D; Lee, J-L.; Lai, CC; Weng, Y-J; Chu, S-Y

doi:10.1046/j.1365-2761.2002.00415.x

Cited by 62 publications

(66 citation statements)

References 16 publications

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“…In Taiwan, lactococcosis has appeared regularly in grey mullet farms from 2002, representing an important risk factor in the grey mullet industry during the summer (Chen et al 2002). This disease has been found in Taiwan in other aquatic animals such as rainbow trout, giant freshwater prawns, tilapia and bullfrogs, necessitating the development of a rapid and sensitive detection method to help limit its further spread.…”

Section: Discussionmentioning

confidence: 99%

“…The PCR assay targeting the 16S rDNA, developed by Zlotkin et al (1998), has been used to identify L. garvieae as described previously (Chen et al 2002, Colorni et al 2003, Kang et al 2004, Wang et al 2007, Madinabeitia et al 2009). The present study compared the sensitivities of both PCR (Zlotkin et al 1998) and LAMP.…”

Section: Discussionmentioning

confidence: 99%

“…(Kusuda et al 1991), amberjack S. dumerili, kingfish S. lalandi (Kawanishi et al 2005), wild wrasse Coris aygula (Colorni et al 2003), black rockfish Sebastes schlegeli (Kang et al 2004), grey mullet Mugil cephalus (Chen et al 2002) and in giant freshwater prawn Macrobrachium rosenbergii (Chen et al 2001). In addition to aquatic animals, L. garvieae has been isolated from other homoiothermic and poikilothemic animals such as cows, buffalos, cats and dogs (Pot et al 1996, Carvalho et al 1997, Devriese et al 1999.…”

Section: Introductionmentioning

confidence: 99%

“…L. garvieae is reported to be the major pathogen in bacterial infections of cultured grey mullet, occurring mainly from May to September in Taiwan. Epizootics with up to 10% mortality were recorded on some Taiwanese grey mullet farms (Chen et al 2002, Lee et al 2006. Additionally, the gross pathology of lactococcosis initially appears with rapid and general anorexia, melanosis, lethargy, loss of orientation and erratic swimming.…”

Section: Introductionmentioning

confidence: 99%

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Development of a sensitive and specific LAMP PCR assay for detection of fish pathogen Lactococcus garvieae

Tsai¹,

Wang²,

Yoshida³

et al. 2013

Dis. Aquat. Org.

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Based on use of a loop-mediated isothermal amplification (LAMP) identification protocol, this study attempted to detect Lactococcus garvieae in fish by using primer sets designed from an L. garvieae alpha/beta fold family hydrolase gene. Reaction time and temperatures were optimized for 60 min at 60°C with the resulting amplicons visualized by adding SYBR Green I to the reaction tube. The assay specificity was assessed using 45 different bacterial strains. Positive results were observed in all 30 L. garvieae isolates from various aquatic animals. No false-positive results were observed in 15 non-L. garvieae strains. The detection limit of the LAMP assay was 10-fold more sensitive than the conventional polymerase chain reaction (PCR) targeting 16S rDNA when using purified L. garvieae DNA. The detection limit of the LAMP assay was approximately 300 colony-forming units (CFU) using crude bacterial lysates, 100-fold more sensitive than PCR. Furthermore, L. garvieae in spleen, kidney and brain of experimentally challenged tilapia and grey mullet were detected using this optimized LAMP assay. Results of this study demonstrate the effectiveness of LAMP in providing a rapid yet simple test for detecting L. garvieae in fish.KEY WORDS: Lactococcosis · Alpha/beta fold family · Hydrolase gene · Loop-mediated isothermal amplification · Polymerase chain reaction · SYBR Green I Resale or republication not permitted without written consent of the publisherDis Aquat Org 102: [225][226][227][228][229][230][231][232][233][234][235] 2013 swimming. However, similar clinical signs are also found in Streptococcus sp. infections (Eldar & Ghittino 1999).Molecular detection methods of Lactococcus garvieae have been developed, including polymerase chain reaction (PCR) (Zlotkin et al. 1998, Aoki et al. 2000, Pu et al. 2002 and 16S rRNA and sodA sequence analysis (Fihman et al. 2006). However, although considered rapid and specific methods for detection, these require expensive equipment and other costly materials (Gunimaladevi et al. 2005).A method of amplifying nucleic acid sequences with high specificity, sensitivity and rapidity under isothermal conditions (60 to 65°C), loop-mediated isothermal amplification (LAMP) has been developed more recently (Notomi et al. 2000) and applied to fish pathogen detection (Caipang et al. 2004, Savan et al. 2004, Yeh et al. 2005, Itano et al. 2006. The assay uses a set of 4 or 6 primers that create a targetspecific stem loop DNA structure during initial amplification steps; subsequent LAMP auto-cycling is performed by the Bst DNA polymerase large fragment (Notomi et al. 2000). Since it does not require complicated equipment, the LAMP method provides a cost-effective, simple, and rapid (producing a result within 60 min) DNA amplification method.Recently, a fosmid library of Lactococcus garvieae from grey mullet has been constructed and many new genes of L. garvieae were cloned (M. A. Tsai et al. unpubl. data). The clone flg08-6 contained an alpha/beta fold family hydrolase gene that is also...

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Development of a sensitive and specific LAMP PCR assay for detection of fish pathogen Lactococcus garvieae

Tsai¹,

Wang²,

Yoshida³

et al. 2013

Dis. Aquat. Org.

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“…Likewise, L. garvieae is an established pathogen of fish, causing a variety of clinical signs and significant mortalities among fish worldwide. Affected fish species include rainbow trout (Oncorhynchus mykiss) (Ravelo et al, 2001;Chang et al, 2002), yellowtail (Seriola quinqueradiata) (Zlotkin et al, 1998a), and grey mullet (Mugil cephalus L.) (Chen et al, 2002). Streptococcal organisms are also of concern to public aquaria, because they affect aquarium species such as danios, Brachydanio rerio and Brachydanio albolineatus, and minnows (Tanichthys albonubes) (Ferguson et al, 1994).…”

Section: Introductionmentioning

confidence: 99%

First Report of Streptococcus Agalactiae and Lactococcus Garvieae From a Wild Bottlenose Dolphin (Tursiops Truncatus)

Evans

Pasnik

Klesius

et al. 2006

Journal of Wildlife Diseases

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ABSTRACT:The isolation and characterization of two bacterial species, Streptococcus agalactiae and Lactococcus garvieae, previously unreported in wild marine mammals are described from a freshly dead bottlenose dolphin, Tursiops truncatus, from Kuwait Bay, Kuwait, in September 2001. Conventional and rapid identification systems were used to determine that isolates from muscle and kidney were S. agalactiae and L. garvieae, respectively. The isolates were grampositive, catalase-negative, oxidase-negative, nonhemolytic cocci. The S. agalactiae was serotyped to group antigen B, whereas the L. garvieae could not be assigned to any serogroup. These Kuwait isolates displayed considerable homogeneity with corresponding American Type Culture Collection (ATCC) type isolates. Although the dolphin S. agalactiae isolate was nonhemolytic, it was biochemically similar to S. agalactiae isolated from mullet sampled in the concurrent Kuwait Bay fish kill. Some biochemical heterogeneity was observed between the dolphin isolates and corresponding mammalian ATCC type isolates, especially with Voges Proskauer, alaninephenylanaline-proline arylamidase, and alpha-galactosidase tests. Nile tilapia, Oreochromis niloticus, experimentally infected with the dolphin S. agalactiae and L. garvieae isolates experienced 90% and 0% mortalities, respectively. This is the first isolation of S. agalactiae and L. garvieae from a wild marine mammal, and the microbial characteristics established here provide pertinent information for the future isolation of these bacteria.

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Vaccination against Streptococcosis and Lactococcosis

Pridgeon

Klesius

2014

Fish Vaccination

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Lactococcus garvieae, a cause of disease in grey mullet, Mugil cephalus L., in Taiwan

Cited by 62 publications

References 16 publications

Development of a sensitive and specific LAMP PCR assay for detection of fish pathogen Lactococcus garvieae

Development of a sensitive and specific LAMP PCR assay for detection of fish pathogen Lactococcus garvieae

First Report of Streptococcus Agalactiae and Lactococcus Garvieae From a Wild Bottlenose Dolphin (Tursiops Truncatus)

Vaccination against Streptococcosis and Lactococcosis

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