<i>Leclercia adecarboxylata</i>Infections: Case Report and Review

Temesgen, Zelalem; Toal, Douglas R.; Cockerill, Franklin R.

doi:10.1086/514514

Cited by 83 publications

(73 citation statements)

References 8 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Conventional biochemical tests were used for the phenotypic identification of aerobic and anaerobic bacteria (22,24). For some nonfermenting aerobic bacteria, the MicroLog system, release 4.0 (Biolog, Inc., Hayward, Calif.), was used.…”

Section: Methodsmentioning

confidence: 99%

“…It has been estimated that there are 500,000 cases of bacteremia each year, with an associated crude mortality of 35% (28). The rapid and reliable detection and subsequent identification of microorganisms from blood remain critical so that the appropriate antimicrobial therapy can be provided.DNA sequencing of conserved regions within phylogenetically informative genetic targets, such as the small-subunit (16S) rRNA gene, is promising as a means for identifying bacteria (5,9,22,27). Broad-range PCR primers can be used to amplify highly conserved regions of the 16S rRNA gene from a wide range of organisms; highly variable regions within the amplicon permit phylogenetic analysis and, in many cases, species-level identification (7, 29).…”

mentioning

confidence: 99%

“…DNA sequencing of conserved regions within phylogenetically informative genetic targets, such as the small-subunit (16S) rRNA gene, is promising as a means for identifying bacteria (5,9,22,27). Broad-range PCR primers can be used to amplify highly conserved regions of the 16S rRNA gene from a wide range of organisms; highly variable regions within the amplicon permit phylogenetic analysis and, in many cases, species-level identification (7, 29).…”

mentioning

confidence: 99%

See 2 more Smart Citations

Direct Identification of Bacteria from Positive Blood Cultures by Amplification and Sequencing of the 16S rRNA Gene: Evaluation of BACTEC 9240 Instrument True- Positive and False-Positive Results

Qian¹,

Tang²,

Kolbert³

et al. 2001

J Clin Microbiol

View full text Add to dashboard Cite

In a previous study which evaluated the BACTEC 9240 automated blood culture system (Becton Dickinson Diagnostic Instrument Systems, Sparks, Md.), we noted a 1.3% "instrument false-positive" rate. That is, the BACTEC system signaled that a bottle (BACTEC Plus Aerobic/F bottle or BACTEC Anaerobic Lytic/10 bottle) culture was positive but a Gram stain was negative and there was no growth of bacteria or yeasts on subculture to chocolate agar. Furthermore, from the same sample of blood, cultures for fungi using the Isolator blood culture system (Wampole Laboratories, Cranbury, N.J.) were negative for growth. For the present study, we evaluated 76 instrument false-positive samples for the presence of 16S ribosomal DNA using the MicroSeq 500 kit (PE Biosystems, Foster City, Calif.). These samples also were negative for fungi by the Isolator method. This kit has a PCR module and sequencing module for the amplification and sequencing of the 16S RNA gene and provides a database for sequence alignment and identification of bacteria. To optimize the assay, we evaluated the effect of adding 0.5% bovine serum albumin to the sample from blood culture bottles and found that it decreased the effects of inhibitors on the PCR. Two control groups of blood culture specimens were also evaluated. One group (n ‫؍‬ 45) were "instrument true positives"; the instrument signaled positive, and subsequent Gram stains were positive and subcultures on chocolate agar grew bacteria. The other group (n ‫؍‬ 20) were "instrument true negatives"; the instrument signaled negative, the Gram stain was negative, and subcultures on chocolate agar and from the Isolator tube on fungal media showed no growth. None of the 76 instrument false-positive samples had evidence for 16S rRNA gene sequences. All of the instrument true-positive samples and all of the instrument true-negative specimens were positive and negative, respectively, using the MicroSeq 500 kit. Total peripheral white blood cell counts were statistically significantly higher for patients who had instrument false-positive results than for patients who had instrument true-positive or true-negative results (P ‫؍‬ 0.001). We conclude that instrument false positives signaled by the BACTEC 9240 system are not due to bacteria in the blood culture samples.Bacteremia has become a major nosocomial infection in most medical centers and represents a formidable source of morbidity and mortality (1,14,19,26). Patients with bloodstream infections have a three-times-greater risk of dying than that expected from the underlying diseases alone (27). It has been estimated that there are 500,000 cases of bacteremia each year, with an associated crude mortality of 35% (28). The rapid and reliable detection and subsequent identification of microorganisms from blood remain critical so that the appropriate antimicrobial therapy can be provided.DNA sequencing of conserved regions within phylogenetically informative genetic targets, such as the small-subunit (16S) rRNA gene, is promising as a means for identifying bacte...

show abstract

Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

See 1 more Smart Citation

Direct Identification of Bacteria from Positive Blood Cultures by Amplification and Sequencing of the 16S rRNA Gene: Evaluation of BACTEC 9240 Instrument True- Positive and False-Positive Results

Qian¹,

Tang²,

Kolbert³

et al. 2001

J Clin Microbiol

View full text Add to dashboard Cite

show abstract

“…Leclercia adecarboxylata is a part of normal flora in the gut of animals and its epidemiological significance is not clear though it has been recovered from cases of wound infection, bacteraemia, sputum samples, urinary tract infections, vaginal discharge. [6][7][8] Most recently it has been reported from India, as the cause of ventilator associated pneumonia and pharyngeal, peri-tonsillar abscess and in immune-competent patients. 9,10 It has most commonly been isolated from different infections as a part of mixed flora and most of the cases were immunecompromised, the clinical significance of which is not clear.…”

Section: Discusssionmentioning

confidence: 99%

“…9,10 It has most commonly been isolated from different infections as a part of mixed flora and most of the cases were immunecompromised, the clinical significance of which is not clear. 6,11 Few reports raised suspicion of association between L. adecarboxylata cutaneous infection and contaminated sea water especially in cases wound infection among healthy individuals. 12 Stock et al studied the natural antimicrobial susceptibility pattern of 101 strains and showed that all of them were naturally sensitive to all tested aminoglycosides.…”

Section: Discusssionmentioning

confidence: 99%

Peritonitis by Leclercia adecarboxylata in a patient with continuous ambulatory peritoneal dialysis: the first case report from India

et al. 2016

View full text Add to dashboard Cite

Leclercia

O'Hara¹,

Farmer²

2015

Bergey's Manual of Systematics of Archaea and Bacteria

View full text Add to dashboard Cite

Le.clerc' i.a . M.L. fem. n. Leclercia named to honor H. Leclerc, a French bacteriologist, who first described and named this organism Escherichia adecarboxylata in 1962, and who made many other contributions to enteric bacteriology. Proteobacteria / Gammaproteobacteria / Enterobacteriales / Enterobacteriaceae / Leclercia Small rod‐shaped cells, conforming to the general definition of the family Enterobacteriaceae . Gram negative. Motile at 36°C and 25°C with peritrichous flagella. Facultatively anaerobic. Catalase positive. Oxidase negative. Many strains produce a nondiffusible yellow pigment that may be weak and may be lost on storage and subculture . Ferment, rather than oxidize, D ‐glucose and other carbohydrates. Reduce nitrate to nitrite. Positive for indole production, methyl red, growth in the presence of cyanide, malonate utilization, esculin hydrolysis, ONPG, and the fermentation of lactose , d ‐ mannitol, dulcitol, salicin, adonitol , l ‐ arabinose , l ‐ rhamnose, maltose , d ‐ xylose, trehalose, cellobiose, erythritol, esculin, melibiose , d ‐ arabitol, mucate , d ‐ mannose, and D ‐galactose. Produce visible gas during fermentation . Negative for Voges–Proskauer, citrate utilization (Simmons), H 2 S production (TSI), phenylalanine deaminase, lysine decarboxylase, arginine dihydrolase, ornithine decarboxylase , gelatin hydrolysis (22°C), lipase (corn oil) production, DNase production, and the fermentation of myo ‐inositol, D ‐sorbitol, α‐methyl‐ D ‐glucoside, and erythritol. Most strains are susceptible to colistin, nalidixic acid, sulfadiazine, gentamicin, streptomycin, kanamycin, tetracycline, chloramphenicol, carbenicillin, cephaloridine, and ampicillin (disk diffusion method on Mueller–Hinton agar); resistant to penicillin . The mol % G + C of the DNA is : 52–55. Type species : Leclercia adecarboxylata (Leclerc 1962) Tamura, Sakazaki, Kosako, and Yoshizaki 1987, 179 (Effective publication: Tamura, Sakazaki, Kosako, and Yoshizaki 1986, 183) ( Escherichia adecarboxylata Leclerc 1962, 737.)

show abstract

Leclercia adecarboxylataInfections: Case Report and Review

Cited by 83 publications

References 8 publications

Direct Identification of Bacteria from Positive Blood Cultures by Amplification and Sequencing of the 16S rRNA Gene: Evaluation of BACTEC 9240 Instrument True- Positive and False-Positive Results

Direct Identification of Bacteria from Positive Blood Cultures by Amplification and Sequencing of the 16S rRNA Gene: Evaluation of BACTEC 9240 Instrument True- Positive and False-Positive Results

Peritonitis by Leclercia adecarboxylata in a patient with continuous ambulatory peritoneal dialysis: the first case report from India

Leclercia

Contact Info

Product

Resources

About