<i>LeERF‐1</i>, a novel AP2/ERF family gene within the B3 subcluster, is down‐regulated by light signals in <i>Lithospermum erythrorhizon</i>

Zhang, W.; Zou, Ailan; Miao, Jianjun; Yin, Yujing; Tian, Runan; Pang, Yan-Jun; Yang, Rongwu; Qi, Jinliang; Yang, Yonghua

doi:10.1111/j.1438-8677.2010.00375.x

Cited by 26 publications

(26 citation statements)

References 20 publications

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“…7a, b). This phenomenon is consistent with that of LeERF-1, as we previously reported (Zhang et al 2010a), indicating that ethylene may be an important regulator involved in the formation of shikonin.…”

Section: Identification Of the Prokaryotically Expressed Leeil-1 Protsupporting

confidence: 93%

“…The samples for LeEIL-1 expression analysis were collected at 0, 3, 6, and 12 hours; 1, 2, 4, and 6 days after the cells were transferred to the M9 production medium (Zhang et al 2010a). The mature seeds of L. erythrorhizon were germinated and grown in a greenhouse, and roots, stems, and leaves of the intact seedlings with 16 true leaves were chosen for tissue-specific expression analysis of LeEIL-1 (Zhang et al 2010a). …”

Section: Characterization and Bioinformatic Analysis Of Leeil-1mentioning

confidence: 99%

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Cloning, characterization, and expression of LeEIL-1, an Arabidopsis EIN3 homolog, in Lithospermum erythrorhizon

Zou

Zhang

Pan

et al. 2010

Plant Cell Tiss Organ Cult

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Ethylene is a crucial signal in regulating the biosynthesis of shikonin in Lithospermum erythrorhizon. Arabidopsis ethylene-insensitive 3 (EIN3) is the key transcriptional factor of the ethylene signal transduction pathway; thus, EIN3 homologs might play an important role in shikonin formation. Here, LeEIL-1, a gene with high similarity to EIN3, was cloned from L. erythrorhizon using a combination method of touch-down PCR and rapid amplification of cDNA ends. The full-length cDNA of LeEIL-1 is 2,359 bp, encoding a polypeptide of 635 amino acids. By inserting the entire coding region of LeEIL-1 into the pET32a (?) expression vector, the prokaryotic expression of LeEIL-1 was successfully induced by isopropyl b-D-1-thiogalactopyranoside in BL21(DE3)pLysS cells. Moreover, the recombinant LeEIL-1 protein was verified by western blotting with the Arabidopsis anti-EIN3 antibody. Real-time PCR results show that the mRNA level of LeEIL-1 was first dramatically induced within 3 h when the L. erythrorhizon cells were transferred from B5 to M9 medium for shikonin formation; subsequently, the transcripts of LeEIL-1 decreased to a relatively stable level. Tissue-specific expression analysis indicates that less LeEIL-1 mRNA accumulated in the stem and leaf than in the root, where shikonin was biosynthesized. These results imply that LeEIL-1 could be a possible reverse genetic target for revealing the relationship between ethylene and shikonin formation.

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Section: Identification Of the Prokaryotically Expressed Leeil-1 Protsupporting

confidence: 93%

Section: Characterization and Bioinformatic Analysis Of Leeil-1mentioning

confidence: 99%

Cloning, characterization, and expression of LeEIL-1, an Arabidopsis EIN3 homolog, in Lithospermum erythrorhizon

Zou

Zhang

Pan

et al. 2010

Plant Cell Tiss Organ Cult

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“…Real-time PCR results showed that LeERF-1 was significantly down-regulated by white, blue and red light, and the inhibitory effect of red light was the weakest compared to other light conditions. These expression patterns were consistent with the content variation of shikonin and its derivatives under different light signal conditions [34].…”

Section: Ap2/erf In Regulation Of Secondary Metabolites In Medicinal supporting

confidence: 84%

Research Advances of AP2/ERF Transcription Factors

Wang¹,

Li²

2018

Proceedings of the 2017 2nd International Conference on Biological Sciences and Technology (BST 2017)

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Abstract. The AP2/ERF transcription factor family is one of the largest transcription factor groups in plants and it plays very important roles in growth, development, biotic and abiotic stress response. In this study, we summarized the research advances on AP2/ERF transcription factor involved in plant growth, development, stress resistance, regulation of secondary metabolites and the current research status in poplar, in order to provide reference for plant-breeding applications. IntroductionTranscription factor (TF), also known as trans-acting factor, is a kind of protein that can specifically bind to the cis-acting element in the promoter of eukaryotes. It plays a role as a switch in initiating downstream gene expression via interaction between transcription factor and proteins [1]. With the completion of genome sequences in plants and the development of bioinformatics analysis, a variety of transcription factors, which are associated with drought, high salinity, cold, hormone, pathogen response, growth and development, have been isolated from higher plants since Paz-Ares first reported the TF from Zea mays in 1987 [2].The AP2/ERF (APETALA 2/ethylene-responsive element binding factor, AP2/ERF) family is one of the largest TF families in plants. It accounts for nearly 9% of the TFs in plants and contains a 58 or 59 amino acid conserved domain. The AP2/ERF TF is widely involved in regulation of growth, development and various stress responses in plant [3]. According to the characteristics and number of AP2/ERF domain, it can be divided into 3 families: AP2, RAV, and ERF. The AP2 family contains two repeated AP2/ERF domains, and this family plays a very important role in plant developmental processes [4]. The RAV family contains one AP2/ERF domain and a B3 domain, which plays an important role in response to ethylene, brassinolide, biotic and abiotic stress [5]. The ERF family contains one AP2/ERF domain and it can be divided into two subfamilies: CBF/DREB subfamily and ERF subfamily. The differences between the two subfamilies are the 14th and 19th amino acid. The 14th and 19th amino acids of DREB are valine and glutamic acid, while the corresponding amino acids in ERF are alanine and aspartic acid. DREB and ERF transcription factors are mainly involved in plant response to hormone [6] and environmental stresses [7].In this review, we analyzed the functions of AP2/ERF TFs in plant growth, development, stress resistance, regulation of secondary metabolites in medicinal plant and the current research status in poplar, in order to provide reference for plant-breeding applications.

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“…For example, DREB1a is a classical regulator of drought and cold tolerance responses in plants (Sakuma et al, 2002;Miura et al, 2007). ERF1 is known to play a role in biotic stress (Lorenzo et al, 2003;Zhang et al, 2011), and RAV1 has been described in context with senescence and different abiotic stress conditions (Sohn et al, 2006;Woo et al, 2010;Yun et al, 2010). It is therefore likely that both MATH overexpression and 6xamiBPM plants display different sensitivities toward stress, such as cold or drought, as well as treatments with phytohormones, such as ethylene or jasmonic acid, in addition to ABA.…”

Section: Discussionmentioning

confidence: 99%

Arabidopsis BPM Proteins Function as Substrate Adaptors to a CULLIN3-Based E3 Ligase to Affect Fatty Acid Metabolism in Plants

Chen

Lee

Weber³

et al. 2013

The Plant Cell

114

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Regulation of transcriptional processes is a critical mechanism that enables efficient coordination of the synthesis of required proteins in response to environmental and cellular changes. Transcription factors require accurate activity regulation because they play a critical role as key mediators assuring specific expression of target genes. In this work, we show that CULLIN3-based E3 ligases have the potential to interact with a broad range of ETHYLENE RESPONSE FACTOR (ERF)/APETALA2 (AP2) transcription factors, mediated by MATH-BTB/POZ (for Meprin and TRAF [tumor necrosis factor receptor associated factor] homolog)-Broad complex, Tramtrack, Bric-a-brac/Pox virus and Zinc finger) proteins. The assembly with an E3 ligase causes degradation of their substrates via the 26S proteasome, as demonstrated for the WRINKLED1 ERF/AP2 protein. Furthermore, loss of MATH-BTB/POZ proteins widely affects plant development and causes altered fatty acid contents in mutant seeds. Overall, this work demonstrates a link between fatty acid metabolism and E3 ligase activities in plants and establishes CUL3-based E3 ligases as key regulators in transcriptional processes that involve ERF/AP2 family members.

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LeERF‐1, a novel AP2/ERF family gene within the B3 subcluster, is down‐regulated by light signals in Lithospermum erythrorhizon

Cited by 26 publications

References 20 publications

Cloning, characterization, and expression of LeEIL-1, an Arabidopsis EIN3 homolog, in Lithospermum erythrorhizon

Cloning, characterization, and expression of LeEIL-1, an Arabidopsis EIN3 homolog, in Lithospermum erythrorhizon

Research Advances of AP2/ERF Transcription Factors

Arabidopsis BPM Proteins Function as Substrate Adaptors to a CULLIN3-Based E3 Ligase to Affect Fatty Acid Metabolism in Plants

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