Listeria monocytogenes mediated CFTR transgene transfer to mammalian cells

Abstract

This is the first demonstration of L. monoyctogenes mediated CFTR transgene transfer. The successful in vitro transfer suggests that L. monocytogenes might be a potential vector for cystic fibrosis gene therapy or alternative applications and deserves further investigation in vitro as well as in vivo.

Involvement of Absent in Melanoma 2 in Inflammasome Activation in Macrophages Infected with Listeria monocytogenes

“…At that time, most of bacteria should have escaped into the cytoplasm (21). Penicillin G rapidly killed intracellular bacteria, as described in a previous report (46), and the number of intracellular L. monocytogenes at its peak was markedly fewer compared with the number of organisms in the absence of penicillin G (Fig. 5A).…”

Bactofection of mammalian cells by Listeria monocytogenes: improvement and mechanism of DNA delivery

“…If the attenuation of this carrier should prove to be too rigorous under in vivo conditions, we can lower its attenuation by modulating the expression of the phage lysin within the host cell cytosol by using different variants of the actA promoter. This live vector system, which seems to be not only suitable for the release of DNA but also of RNA (C Schoen et al, unpublished results) and of biologically active proteins (J Stritzker et al, unpublished results), may thus be applicable without risk in genetic therapy approaches 17,18 and vaccine development against infectious agents and tumors. 31 Experimental protocol…”

“…16 One already reported example is the transfer of the human cystic fibrosis transmembrane conductance regulator (cftr) into epithelial cells, which recently was shown by using L. monocytogenes as carrier. 17 Likewise, Salmonella typhimurium were used to deliver genes encoding therapeutic proteins like IL-12 or GM-CSF to achieve antitumor effects in mice. 18 The use of bacterial systems for gene therapy has the added advantage that bacteria often have an innate tropism for specific target tissues.…”

Bactofection of lung epithelial cells in vitro and in vivo using a genetically modified Escherichia coli

“…13,14,[17][18][19] These studies have mainly focused on transfer of the GFP reporter gene. However, more relevant to our work, recombinant E. coli and L. monocytogenes were recently used to transfer artificial chromosomes carrying the CFTR gene locus 20 or a pCMV-CFTR plasmid, 10,21 respectively, to cell lines in vitro.…”