Lycium barbarum polysaccharides promoted proliferation and differentiation in osteoblasts

Abstract

Osteoblasts have the capacity to differentiate into several different cell types, including adipocyte, chondrocyte, and muscle lineages. Therefore, osteoblast can be potentially applied in the treatment of bone diseases. The factors controlling osteoblast differentiation is complex. Recently, it has been reported that some natural products regulate the differentiation in osteoblasts and promote bone formation. Based on these findings, this study demonstrated that Lycium barbarum polysaccharides (LBP) could promote proliferation of osteoblast MC3T3‐E1 cells through 3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyltetrazolium bromide (MTT) assay. Besides, expression of key proteins correlated with cellular proliferation such as proliferating cell nuclear antigen (PCNA) and Ki67 was enhanced in the presence of LBP. We also detected the increased expression of bone‐specific matrix proteins such as morphogenetic protein 2 (BMP2), bone Gla protein (BGP), osteopontin (OPN), and α‐1 type‐I collagen (COL1A1) when treated with LBP. This process was mediated by some signals, such as smad1, smad8, Runt‐related transcription factor 2 (RUNX2), and Osterix. Furthermore, RUNX2 silencing inhibited osteoblast differentiation by decreasing expression of bone‐specific matrix proteins. Collectively, we proposed a previously unidentified function of LBP in osteoblast differentiation, suggesting its potential clinical role in bone disease treatment.

Molecular insights for an anti-osteoporotic properties of Litsea glutinosa on Saos-2 cells: An in-vitro approach

“…Thus, upregulation of PCNA and OCN along with morphological observation clearly indicate the proliferative effects of LG. These results are in accordance with the previous studies on increased proliferation with number of herbs [ 54 , 55 ].…”

Lycium barbarum polysaccharide‐glycoprotein promotes osteogenesis in hPDLSCs via ERK activation

“…In addition, many studies have shown that LBP can reduce the damage of free radicals to the organism by reducing the number of free radicals and enhancing the activity of antioxidant enzymes in the process of damage repair. Researchers have demonstrated that LBP can stimulate osteoblast proliferation (Chan et al, 2019), increase the expression of key proteins linked to cellular proliferation such as proliferating cell nuclear antigen (PCNA) and Ki67, and can also enhance the activation of osteogenesis‐related proteins including bone morphogenetic protein 2 (BMP2), bone gamma‐carboxyglutamate protein (BGP), secreted phosphoprotein 1 (OPN), and collagen type I alpha 1 chain (COL1A1) (H. Y. Zhang et al, 2019).…”

Antiosteoporosis Studies of 20 Medicine Food Homology Plants Containing Quercetin, Rutin, and Kaempferol: TCM Characteristics, In Vivo and In Vitro Activities, Potential Mechanisms, and Food Functions

“…The above three samples were tested on calvarial osteoblasts from the rat/mice calvaria at postnatal day. The polysaccharides from L. barbarum [ 28 ], and three compounds including crocin [ 34 ], linarin via bone morphogenetic proteins 2(BMP-2)/runt-related transcription factor 2（Runx2） pathway [ 42 ], and ginsenoside Rb2 via a reduction of oxidative damage [ 20 ] were all tested on the osteoblast MC3T3‐E1 cell line. The results showed that osteoblast differentiation could be induced by the above extracts and compounds [ 18 , 20 , 28 , 30 , 33 , 34 , 42 ].…”