AbstractInterest has grown in harnessing biological agents for cancer treatment as dynamic and responsive vectors with enhanced tumor targeting. While bacterial traits such as proliferation in tumors, modulation of an immune response, and local secretion of toxins have been well studied, less is known about bacteria as competitors for nutrients. Here, we investigated the use of a bacterial strain as a living iron chelator, competing for this nutrient vital to tumor growth and progression. We established an in vitro co-culture system consisting of the magnetotactic strain Magnetospirillum magneticum AMB-1 incubated under hypoxic conditions with human melanoma cells. Siderophores produced by 108 AMB-1/mL in human transferrin (Tf)-supplemented media were found to alter the Tf structure with an effect equivalent to 3.78 μM ± 0.117 μM deferoxamine, a potent drug used in iron chelation therapy. Our experiments revealed an increased expression of transferrin receptor 1 (TfR1), indicating the bacteria’s ability to influence iron homeostasis in human melanoma cells. Our results show the potential of a bacterial strain as a self-replicating iron-chelating agent, which could serve as an additional mechanism reinforcing current bacterial cancer therapies.