<i>mda-7/IL-24</i> Mediates Cancer Cell–Specific Death via Regulation of miR-221 and the Beclin-1 Axis

Pradhan, Anjan K.; Talukdar, Sarmistha; Bhoopathi, Praveen; Shen, Xue‐Ning; Emdad, Luni; Das, Swadesh K.; Sarkar, Devanand; Fisher, Paul B.

doi:10.1158/0008-5472.can-16-1731

Cited by 48 publications

(63 citation statements)

References 49 publications

(62 reference statements)

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“…IL‐24 was also found to be regulated by miR‐203 but the type of miR‐203 was not mentioned . In another report, it was shown that IL‐24 regulates miR‐221 expression in cancer cells . In this study, we identified that IL‐24 was regulated by miR‐203a‐3p.1 in HCC cells.…”

Section: Discussionmentioning

confidence: 60%

miR‐203a‐3p.1 targets IL‐24 to modulate hepatocellular carcinoma cell growth and metastasis

Huo

Pan

et al. 2017

FEBS Open Bio

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Hepatocellular carcinoma ( HCC ) is one of the most common causes of cancer‐related death. Cytokines, including interleukin 24 ( IL ‐24), play an important role in HCC . IL ‐24 inhibits HCC metastasis but the molecular mechanism by which this occurs is still unknown. Micro RNA s (mi RNA s) are regulators of cancers including hepatocellular carcinoma ( HCC ). However, the role that mi RNA s play in the regulation of IL ‐24 in HCC is unclear. The aim of this study was to investigate the effects of regulation of IL‐24 by miR‐203a‐3p.1 on liver cancer cell proliferation and metastasis. IL ‐24 mRNA and miR‐203a‐3p.1 were detected by real‐time RT ‐ PCR , and IL ‐24 protein in the cell growth medium was measured by ELISA . A luciferase assay was used to verify that the IL ‐24 gene was the target of miR‐203a‐3p.1. Cell survival ability was detected by the MTT assay and colony formation. Cell metastasis was assayed by the Transwell system. The results showed that IL ‐24 could be down‐regulated by miR‐203a‐3p.1 in HCC cells and that miR‐203a‐3p.1 acted as an onco‐mi RNA by targeting IL ‐24. Inhibition of miR‐203a‐3p.1 in cells could lead to the reversal of HCC cell proliferation and metastasis. The study highlights a novel molecular interaction between miR‐203a‐3p.1 and IL ‐24, which indicates that IL ‐24 and miR‐203a‐3p.1 may constitute potential therapeutic targets for HCC .

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Section: Discussionmentioning

confidence: 60%

miR‐203a‐3p.1 targets IL‐24 to modulate hepatocellular carcinoma cell growth and metastasis

Huo

Pan

et al. 2017

FEBS Open Bio

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“…MiR-221 has been considered to act as a tumor regulator [26,27]. In recent years, studies have found that miR-221 is associated with cardiovascular disease.…”

Section: Discussionmentioning

confidence: 99%

MiR-221 is involved in depression by regulating Wnt2/CREB/BDNF axis in hippocampal neurons

et al. 2018

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Objective: The aim of this study was to investigate the mechanism of miR-221 in depression. Methods: The molecules expressions were measured by qRT-PCR and western blot. The sucrose preference test (SPT), forced swimming test (FST) and tail suspension test (TST) were used to detect depressive-like symptoms. MTT assay and flow cytometric was used to measure the proliferation and apoptosis of hippocampal neuronal. Results: MiR-221 expression in the cerebrospinal fluid and serum of major depressive disorder patients and the hippocampus of chronic unpredictable mild stress (CUMS) mice were increased, while the expression of Wnt2, p-CREB and BDNF were decreased. Additionally, silence of miR-221 increased sucrose preference of CUMS mice and shortened the immobility time of CUMS mice in SPT and FST. MiR-221 could targeted regulate Wnt2, and knockdown of Wnt2 reversed the effect of miR-221 inhibitor on the proliferation and apoptosis of hippocampal neurons and countered the promoting effect of miR-221 inhibitor on the expression of Wnt2, p-CREB and BDNF. Conclusion: MiR-221 could promote the development of depression by regulating Wnt2/CREB/ BDNF axis. ARTICLE HISTORY

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“…Another recent study from our group demonstrates that overexpression of mda‐7/IL‐24 , either via Ad. or as a recombinant protein, downregulates miR‐221 and upregulates p27 and PUMA, resulting in cancer cell death (Pradhan et al, ). This study also identified beclin‐1 as a new transcriptional target of miR‐221 and showed that mda‐7/IL‐24 regulates autophagy through miR‐221/beclin‐1 feedback (Pradhan et al, ).…”

Section: Mda‐7/il‐24: Novel Multifunctional Cancer Therapeutic Cytokinementioning

confidence: 99%

Cancer terminator viruses (CTV): A better solution for viral‐based therapy of cancer

Emdad¹,

Das²,

Wang³

et al. 2018

Journal Cellular Physiology

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In principle, viral gene therapy holds significant potential for the therapy of solid cancers. However, this promise has not been fully realized and systemic administration of viruses has not proven as successful as envisioned in the clinical arena. Our research is focused on developing the next generation of efficacious viruses to specifically treat both primary cancers and a major cause of cancer lethality, metastatic tumors (that have spread from a primary site of origin to other areas in the body and are responsible for an estimated 90% of cancer deaths). We have generated a chimeric tropism-modified type 5 and 3 adenovirus that selectively replicates in cancer cells and simultaneously produces a secreted anti-cancer toxic cytokine, melanoma differentiation associated gene-7/Interleukin-24 (mda-7/IL-24), referred to as a Cancer Terminator Virus (CTV) (Ad.5/3-CTV). In preclinical animal models, injection into a primary tumor causes selective cell death and therapeutic activity is also observed in non-injected distant tumors, that is, "bystander anti-tumor activity." To enhance the impact and therapeutic utility of the CTV, we have pioneered an elegant approach in which viruses are encapsulated in microbubbles allowing "stealth delivery" to tumor cells that when treated with focused ultrasound causes viral release killing tumor cells through viral replication, and producing and secreting MDA-7/IL-24, which stimulates the immune system to attack distant cancers, inhibits tumor angiogenesis and directly promotes apoptosis in distant cancer cells. This strategy is called UTMD (ultrasound-targeted microbubble-destruction). This novel CTV and UTMD approach hold significant promise for the effective therapy of primary and disseminated tumors.

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mda-7/IL-24 Mediates Cancer Cell–Specific Death via Regulation of miR-221 and the Beclin-1 Axis

Cited by 48 publications

References 49 publications

miR‐203a‐3p.1 targets IL‐24 to modulate hepatocellular carcinoma cell growth and metastasis

miR‐203a‐3p.1 targets IL‐24 to modulate hepatocellular carcinoma cell growth and metastasis

MiR-221 is involved in depression by regulating Wnt2/CREB/BDNF axis in hippocampal neurons

Cancer terminator viruses (CTV): A better solution for viral‐based therapy of cancer

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