<i>Microbacterium</i>

Suzuki, Ken‐ichiro; Hamada, Moriyuki

doi:10.1002/9781118960608.gbm00104

Cited by 5 publications

(6 citation statements)

References 77 publications

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“…Galactose and rhamnose are common within the genus Microbacterium , while glucose and ribose are present in only a few species. In addition, strain 2C T showed a positive glycolate test, indicating a glycolyl-type cell wall, consistent with the one reported for the genus Microbacterium [2].…”

Section: Physiology and Chemotaxonomysupporting

confidence: 87%

“…The polar lipids include diphosphatidylglycerol, phosphatidylglycerol and one or more unidentified glycolipids. The genomic DNA G+C contents range between 63 and 75 mol% [2]. The genus was classified into the family Microbacteriaceae , order Microbacteriales , class Actinomycetia , and the type species is Microbacterium lacticum [3].…”

Section: Introductionmentioning

confidence: 99%

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Microbacterium paulum sp. nov., isolated from microfiltered milk

Bellassi

Fontana

Callegari

et al. 2021

International Journal of Systematic and Evolutionary Microbiology

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A novel Gram-stain-positive, strictly aerobic, short rod-shaped bacterium, designated 2CT, was isolated from freshly packaged microfiltered milk. This strain was able to grow within the NaCl concentration range of 0–5 % (w/v), temperature range of 8–37 °C (optimally at 30 °C) and at pH 6.0–10.0. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain 2CT was closely related to species of the genus Microbacterium , with the highest sequence similarity (99.2 %) to Microbacterium lacticum DSM 20427T as well as Microbacterium flavum DSM 18909T (=YM18-098T). The phylogenetic tree based on 16S rRNA genes showed that strain 2CT clustered with M. flavum DSM 18909T. However, the phylogenetic tree based on concatenated 16S rRNA and four housekeeping genes showed that strain 2CT clustered with M. lacticum DSM 20427T. Furthermore, the phylogenomic tree showed that strain 2CT clustered with M. lacticum DSM 20427T and M. flavum DSM 18909T. The major respiratory quinones were MK-10, MK-11 and MK-12. The predominant cellular fatty acids were anteiso-C15 : 0, iso-C16 : 0 and anteiso-C17 : 0. The polar lipid composition of strain 2CT consisted of diphosphatidylglycerol, phosphatidylglycerol, three unidentified glycolipids and two unidentified lipids. The cell-wall peptidoglycan type was a variant of B1α {Gly} [l-Lys] d-Glu-l-Lys, with the amino acids lysine, glycine, alanine and glutamic acid. The whole-cell sugars consisted of galactose, glucose, ribose and minor amounts of rhamnose. In addition, strain 2CT showed a glycolyl-type cell wall. The genomic DNA G+C content was 69.8mol%, while the average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values with the closely related Microbacterium species were below the recognized thresholds of 95–96 % ANI and 70 % DDH for species definition. Based on the phenotypic and genotypic data, strain 2CT (=LMG 32277T=CECT 30329T) is considered to represent a new species, for which the name Microbacterium paulum sp. nov. is proposed.

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Section: Physiology and Chemotaxonomysupporting

confidence: 87%

Section: Introductionmentioning

confidence: 99%

Microbacterium paulum sp. nov., isolated from microfiltered milk

Bellassi

Fontana

Callegari

et al. 2021

International Journal of Systematic and Evolutionary Microbiology

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“…lindanitolerans JCM 30493 T [9]. The peptidoglycan of strains HY60 T and HY82 T was of B type containing ornithine, glycine, alanine and glutamic acid with ornithine as the diagnostic diamino acid and the acyl type of the muramic acid was glycolyl, in line with the features of the genus Microbacterium [41]. Sugars rhamnose and ribose were detected in both type strains, different from the galactose and ribose in M.…”

Section: Chemotaxonomy Featuresmentioning

confidence: 81%

“…Strains HY60 T and HY82 T had MK-11 (34.9, 45.0 %, respectively) and MK-12 (44.3, 28.6 %, respectively) as the predominant respiratory quinones, which is a typical characteristic of genus Microbacterium [2, 41]. Strains HY60 T and HY82 T also had a certain amount of MK-9 (10.8, 12.9 %, respectively), which was absent in the reference strains M.…”

Section: Chemotaxonomy Featuresmentioning

confidence: 99%

Microbacterium chengjingii sp. nov. and Microbacterium fandaimingii sp. nov., isolated from bat faeces of Hipposideros and Rousettus species

Zhou

Huang

Yang

et al. 2021

International Journal of Systematic and Evolutionary Microbiology

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Four aerobic, Gram-stain-positive, rod-shaped bacteria (HY60T, HY54, HY82T and HY89) were isolated from bat faeces of Hipposideros and Rousettus species collected in PR China. Phylogenetic analyses based on 16S rRNA gene sequences indicated that the four novel strains formed two separate but adjacent subclades close to Microbacterium agarici CGMCC 1.12260T (97.6–97.7 % similarity), Microbacterium humi JCM 18706T (97.3–97.5 %) and Microbacterium lindanitolerans JCM 30493T (97.3–97.4 %). The 16S rRNA gene sequence similarity was 98.3 % between strains HY60T and HY82T, and identical within strain pairs HY60T/HY54 and HY82T/HY89. The DNA G+C contents of strains HY60T and HY82T were 61.9 and 63.3 mol%, respectively. The digital DNA–DNA hybridization and average nucleotide identity values between each novel strain and their closest relatives were all below the 70 % and 95–96 % thresholds for species delimitation, respectively. All four novel strains contained anteiso-C15 : 0, anteiso-C17 : 0, iso-C16 : 0 and iso-C15 : 0 as the main fatty acids, MK-11 and MK-12 as the major respiratory quinones, and diphosphatidylglycerol, phosphatidylglycerol and one unidentified glycolipid as the predominant polar lipids. The cell-wall peptidoglycan was of B type and contained alanine, glutamate, glycine and ornithine. The acyl type of the muramic acid was glycolyl. The whole-cell sugars were rhamnose and ribose. Based on the foregoing polyphasic analyses, it was concluded that the four uncharacterized strains represented two novel species of the genus Microbacterium , for which the names Microbacterium chengjingii sp. nov. [type strain HY60T (=CGMCC 1.17468T=GDMCC 1.1951T=KACC 22102T)] and Microbacterium fandaimingii sp. nov. [type strain HY82T (=CGMCC 1.17469T=GDMCC 1.1949T=KACC 22101T)] are proposed, respectively.

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“…These observations indicated important, differential and extra-hydrocarbonoclastic contributions of Cyanobacteria in C samples. Furthermore, Microbacterium, which is known to be a stringent chemoorganotroph 64 , was identified to be differentially abundant in A samples (Fig. 4, Supplementary Table S2).…”

Section: Discussionmentioning

confidence: 99%

Metagenomic Characteristics of Bacterial Response to Petroleum Hydrocarbon Contamination in Diverse Environments as Revealed by Functional Taxonomic Strategies

Mukherjee

Chettri

Langpoklakpam

et al. 2016

Preprint

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18 Microbial remediation of oil polluted habitats remains one of the foremost methods for 19 restoration of petroleum hydrocarbon contaminated environments. The development of 20 effective bioremediation strategies however, require an extensive understanding of the 21 36 37 38 39 40 41 42 43 48 during production, operational use, and transportation. The development, effectiveness and 49 availability of technologies and strategies pose a significant challenge for the remediation, 50 rehabilitation and restoration of these contaminated environments. Many of the 51 technologies developed and in use for the restoration of oil contaminated environments 52 exploit the potential of biological systems, in particular microbial systems, to use these toxic 53 compounds as substrates for growth. Hence, much of the research conducted on 54 bioremediation has concentrated on the capabilities of a single or couple of microbes 55 exhibiting robust and effective growth using petroleum hydrocarbons. However, in the 56 environment, bioremediation is often a complex process involving co-metabolism, cross-57 induction, inhibition and non-interaction among microbes 1-3 , possibly as petroleum 58 hydrocarbons are a mixture of organic pollutants and therefore are used differently by 59 different microbes. These findings, along with others, established bioremediation as a 60 process mediated by a consortium of microbes rather than a few. Thus, characterization of 61 microbial communities of oil contaminated environments could potentially provide 62 guidelines for effective remediation and restoration of such environments. 63 Until recently, it was only possible to study a handful of microorganisms of interest 64 isolated from source materials (as blood, soil, water or air), given the restrictions of the 65 composition of culture media which cannot reflect and mimic the dynamic nutrient fluxes of 66 the source environment. Indeed, only 1% of microorganisms were found to be cultivable 67 4 using a set of media from the highly characterized soil rhizosphere 4 . The advent of high 68 throughput massively-parallel sequencing methods has however, allowed us to investigate 69 the entire complement of organisms inhabiting a certain environment. These next-70 generation sequencing methods (NGS) include a variety of methods to holistically study any 71 biological system such as amplicon sequencing (for variant identification and phylogenetic 72 surveys), whole genome shotgun sequencing (single organism genome and metagenomes) 73 and RNA-Seq (transcriptomes, metatranscriptomes and identification of non-regular RNAs). 74 These powerful methods have ushered in rapid advances in bioinformatics approaches 75 leading to development of software capable of handling huge amounts of data and offering 76 meaningful biological interpretations of the same. Although a technological breakthrough in 77 modern science, a number of NGS methods as metagenomic and transcriptomic/ 78 metatranscriptomic sequencing are still expensive and hence, most studies on ecologic...

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Microbacterium

Cited by 5 publications

References 77 publications

Microbacterium paulum sp. nov., isolated from microfiltered milk

Microbacterium paulum sp. nov., isolated from microfiltered milk

Microbacterium chengjingii sp. nov. and Microbacterium fandaimingii sp. nov., isolated from bat faeces of Hipposideros and Rousettus species

Metagenomic Characteristics of Bacterial Response to Petroleum Hydrocarbon Contamination in Diverse Environments as Revealed by Functional Taxonomic Strategies

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