<i>Mycobacterium vaccae</i>induces a strong Th1 response that subsequently declines in C57BL/6 mice

Zhang, Lijiao; Jiang, Yanlong; Cui, Ziyin; Yang, Wentao; Liu, Yue; Ma, Yingcong; Shi, Shaohua; Wang, Chunfang; Wang, Chunfeng; Ai-dong, Qian

doi:10.4142/jvs.2016.17.4.505

Cited by 14 publications

(14 citation statements)

References 38 publications

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“…The long-term, Treg-dependent protection from stress-induced exaggeration of spontaneous colitis and anxiety-like defensive behavioral responses in the CSC paradigm (measured 4 weeks after the final immunization with heat-killed M. vaccae NCTC 11659) are consistent with the long-term, Treg-dependent protection from allergic airway inflammation in a murine model of allergic asthma (Zuany-Amorim et al, 2002a,b). Finally, the time course of protective effects of M. vaccae NCTC 11659, in the context of spontaneous colitis and anxiety-like defensive behavioral responses (Reber et al, 2016b), are consistent with the time course of induction of Treg following infection of mice with a live strain of M. vaccae isolated from bovine submaxillary lymph nodes, in which CD4 + CD25 + Foxp3 + Treg are elevated within 1 week of infection, reach maximal levels 3 or 4 weeks following infection, and remain elevated until at least 8 weeks following infection (Zhang et al, 2016).…”

Section: Effects Of M Vaccae Nctc 11659 On the Host Brainstem Serotosupporting

confidence: 64%

Effects of Immunization With the Soil-Derived Bacterium Mycobacterium vaccae on Stress Coping Behaviors and Cognitive Performance in a “Two Hit” Stressor Model

et al. 2021

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Previous studies demonstrate that Mycobacterium vaccae NCTC 11659 (M. vaccae), a soil-derived bacterium with anti-inflammatory and immunoregulatory properties, is a potentially useful countermeasure against negative outcomes to stressors. Here we used male C57BL/6NCrl mice to determine if repeated immunization with M. vaccae is an effective countermeasure in a “two hit” stress exposure model of chronic disruption of rhythms (CDR) followed by acute social defeat (SD). On day –28, mice received implants of biotelemetric recording devices to monitor 24-h rhythms of locomotor activity. Mice were subsequently treated with a heat-killed preparation of M. vaccae (0.1 mg, administered subcutaneously on days –21, –14, –7, and 27) or borate-buffered saline vehicle. Mice were then exposed to 8 consecutive weeks of either stable normal 12:12 h light:dark (LD) conditions or CDR, consisting of 12-h reversals of the LD cycle every 7 days (days 0–56). Finally, mice were exposed to either a 10-min SD or a home cage control condition on day 54. All mice were exposed to object location memory testing 24 h following SD. The gut microbiome and metabolome were assessed in fecal samples collected on days –1, 48, and 62 using 16S rRNA gene sequence and LC-MS/MS spectral data, respectively; the plasma metabolome was additionally measured on day 64. Among mice exposed to normal LD conditions, immunization with M. vaccae induced a shift toward a more proactive behavioral coping response to SD as measured by increases in scouting and avoiding an approaching male CD-1 aggressor, and decreases in submissive upright defensive postures. In the object location memory test, exposure to SD increased cognitive function in CDR mice previously immunized with M. vaccae. Immunization with M. vaccae stabilized the gut microbiome, attenuating CDR-induced reductions in alpha diversity and decreasing within-group measures of beta diversity. Immunization with M. vaccae also increased the relative abundance of 1-heptadecanoyl-sn-glycero-3-phosphocholine, a lysophospholipid, in plasma. Together, these data support the hypothesis that immunization with M. vaccae stabilizes the gut microbiome, induces a shift toward a more proactive response to stress exposure, and promotes stress resilience.

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Section: Effects Of M Vaccae Nctc 11659 On the Host Brainstem Serotosupporting

confidence: 64%

Effects of Immunization With the Soil-Derived Bacterium Mycobacterium vaccae on Stress Coping Behaviors and Cognitive Performance in a “Two Hit” Stressor Model

et al. 2021

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“…In DAR‐901 clinical trials, where enhanced protection was observed compared with BCG, the amount of cytokine produced was no higher than in BCG vaccination 38 . Alternatively, a marked increase in the number of polyfunctional T cells has been attributed to the protective capacity of some Mtb vaccines candidates 9–11,43 …”

Section: Discussionmentioning

confidence: 99%

“…The poorly characterized mechanism of protection achieved through BCG vaccination in children, and the lack of natural sterilizing immunity following Mtb infection have hindered the identification of a correlate of protection, further complicating Mtb vaccine development. While there is no definitive correlate of protection, Mtb vaccines candidates have been postulated to elicit protection through mechanisms such as the development of peptide‐specific T CM (central memory) and T RM (resident memory) cells, 8 and induction of T cells that simultaneously produce tumor necrosis factor‐α (TNF‐α), interferon‐γ (IFNγ), interleukin (IL)‐2 and IL‐17 and combinations thereof 9–11 …”

Section: Introductionmentioning

confidence: 99%

Intranasal immunization with Ag85B peptide 25 displayed on Lactococcuslactis using the PilVax platform induces antigen‐specific B‐ and T‐cell responses

Blanchett

Tsai

Sandford

et al. 2021

Immunol. Cell Biol.

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Mycobacterium tuberculosis (Mtb) remains a global epidemic despite the widespread use of Bacillus Calmette–Guérin (BCG). Consequently, novel vaccines are required to facilitate a reduction in Mtb morbidity and mortality. PilVax is a peptide delivery strategy for the generation of highly specific mucosal immune responses and is based on the food‐grade bacterium Lactococcus lactis that is used to express selected peptides engineered within the Streptococcus pyogenes M1T1 pilus, allowing for peptide amplification, stabilization and enhanced immunogenicity. In the present study, the dominant T‐cell epitope from the Mtb protein Ag85B was genetically engineered into the pilus backbone subunit and expressed on the surface of L. lactis. Western blot and flow cytometry confirmed formation of pilus containing the peptide DNA sequence. B‐cell responses in intranasally vaccinated mice were analyzed by ELISA while T‐cell responses were analyzed by flow cytometry. Serum titers of peptide‐specific immunoglobulin (Ig) G and IgA were detected, confirming that vaccination produced antibodies against the cognate peptide. Peptide‐specific IgA was also detected across several mucosal sites sampled. Peptide‐specific CD4+ T cells were detected at levels similar to those of mice immunized with BCG. PilVax immunization resulted in an unexpected increase in the numbers of CD3+CD4–CD8– [double negative (DN)] T cells in the lungs of vaccinated mice. Analysis of cytokine production following stimulation with the cognate peptide showed the major cytokine producing cells to be CD4+ T cells and DN T cells. This study provides insight into the antibody and peptide‐specific cellular immune responses generated by PilVax vaccination and demonstrates the suitability of this vaccine for conducting a protection study.

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“…Par conséquent, M. vaccae pourrait protéger les souris contre l'infection par M. tuberculosis et améliorer l'immunité à médiation cellulaire innée et adaptative de la souris, suggérant que M. vaccae est un agent immunothérapeutique potentiel dans la tuberculose pulmonaire (Xu et al, 2009). Un constat similaire a été rapporté par l'étude menée par Zhang et al, 2016 montrant que des injections contenant M. vaccae tuée par la chaleur peut provoquer une augmentation des réponses de lymphocytes T régulateurs et T auxiliaires de type Th1, ce qui peut protéger l'hôte contre l'infection tuberculeuse (Zhang et al, 2016).…”

Section: -Tuberculoseunclassified

Apport de Mycobacterium vaccae dans la Prévention et le Traitement de Certaines Maladies : Revue Bibliographique

Zahir

Karym

Ouatat

et al. 2019

ESJ

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Ces dernières décennies, le rôle de Mycobacterium vaccae, une bactérie non pathogène et vivant naturellement dans le sol, comme modulatrice de l'immunité a été évoqué par plusieurs investigations. Ainsi, son apport dans la prévention et le traitement de nombreuses maladies telles que la tuberculose, la lèpre, l'allergie et l'asthme dans lesquelles l'immunité occupe une grande place a été mis en évidence. De telles pathologies présentent un fardeau pour la santé humaine et exigent pour leur traitement et surveillance l'administration des médicaments possédant une pléthore d'effets secondaires. Dans ce sens, l'effet des préparations à la base de Mycobacterium vaccae administrées sous différentes formes a été évalué sur des modèles murins ou dans des essaies cliniques. Il semble, dans le cas des maladies inflammatoires par exemple, que cette mycobactérie renforce l'immunité par l'augmentation de la réponse immunitaire de type T auxiliaires 1 (Th1), l'induction des lymphocytes T régulateurs, menant par la suite à la suppression de cellules de type TH2 et leurs médiateurs impliqués dans l'apparition de l'inflammation ce qui entraine, par conséquent, l'atténuation des symptômes pathologiques. Subséquemment, la présente synthèse bibliographique a pour objectif d'exposer l'impact de la bactérie en question pour lutter contre certaines maladies. Mots-clés :Agent immuno-thérapeutique, lutte contre les maladies, modulation de l'immnité, Mycobacterium vaccae, réponse immunitaire de type T auxiliaires 1

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Mycobacterium vaccaeinduces a strong Th1 response that subsequently declines in C57BL/6 mice

Cited by 14 publications

References 38 publications

Effects of Immunization With the Soil-Derived Bacterium Mycobacterium vaccae on Stress Coping Behaviors and Cognitive Performance in a “Two Hit” Stressor Model

Effects of Immunization With the Soil-Derived Bacterium Mycobacterium vaccae on Stress Coping Behaviors and Cognitive Performance in a “Two Hit” Stressor Model

Intranasal immunization with Ag85B peptide 25 displayed on Lactococcuslactis using the PilVax platform induces antigen‐specific B‐ and T‐cell responses

Apport de Mycobacterium vaccae dans la Prévention et le Traitement de Certaines Maladies : Revue Bibliographique

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