<i>Mycoplasma laidlawii</i>in Human Burns

Markham, Jean G.; Markham, N. P.

doi:10.1128/jb.98.2.827-828.1969

Cited by 19 publications

(5 citation statements)

References 9 publications

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“…Studies have shown that M. gallisepticum induced a more severe disease during dual infection with viruses in avian hosts (19,32). Pertinent to our results, A. Iaidlawii has been isolated directly from the human oral cavity (33), leukemic bone marrow (18), and human burns (29). However, this organism has not as yet been examined with regard to its interaction with viral infections in humans and other animals.…”

Section: Min (V) Activity Against Both Vsv and Sindbis Viruses In Flsupporting

confidence: 71%

Mycoplasma-Associated Induction of Interferon in Ovine Leukocytes

et al. 1973

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A mycoplasmal species, Acholeplasma laidlawii, isolated as a contaminant from a fetal lamb kidney cell line, was shown to be associated with the induction of interferon in cultures of ovine peripheral blood leukocytes. Broth cultures of the mycoplasma induced between 20 and 230 U of interferon per ml in leukocytes from two adult ewes. The amount of interferon produced correlated with the inoculum size of mycoplasma. Interferon production was associated with replication of the mycoplasma in the leukocyte cultures. Interferon was not induced by sterile mycoplasmal broth, a cell-free filtrate of the mycoplasmal cultures, or heat-inactivated mycoplasmas. The antiviral substance was characterized as interferon by the usual criteria.

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Section: Min (V) Activity Against Both Vsv and Sindbis Viruses In Flsupporting

confidence: 71%

Mycoplasma-Associated Induction of Interferon in Ovine Leukocytes

et al. 1973

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“…The saprophytic strain M. laidlawii (75), now reclassified as Acholeplasma laidlawii because of its nonrequirement for sterols for growth, and other sterol nonrequirers such as A. granularum (196) and as yet unclassified species (197,198) are becoming increasingly common cell-culture contaminants. The nonhuman origin of these mycoplasmas raises questions as to the source of these contaminants, although the degree of host specificity of mycoplasmas is itself in question since swine mycoplasmas (75) and A. laidlawii (75,110,149) have been isolated directly from human clinical material. Constitutents of cell-culture medium, such as serum and trypsin, have been frequently tested for the presence of mycoplasmas, with negative results.…”

Section: Sources Of Mycoplasma Contami-mentioning

confidence: 99%

Mycoplasmas and cell cultures.

Stanbridge¹

1971

Bacteriological Reviews

118

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Such terms are now in disfavor and the correct generic name, Mycoplasma, should be used. Recently the mycoplasmas have been classified into two separate families, the sterol-requiring Mycoplasmataceae and the sterol-nonrequiring Acholeplasmataceae (44). To the present time, over 30 named species of mycoplasmas have been included within the two families. In addition there are several unidentifiable isolates (L. Hayflick, personal communication). The above classification refers to classic (large-colony) mycoplasmas. It does not include T-strain mycoplasmas (so called, because they form tiny colonies approximately one-fourth the size of classic mycoplasma colonies) on agar. T-strain mycoplasmas were first isolated by Shepard from human urino-genital tracts (170). They have now been isolated from various animals. The T-strain group, as a whole, is unclassified at the present time. A unique requirement for urea (171), in addition to sterol requirement, for growth of these mycoplasmas has prompted the suggestion that they should be included as a separate genus in the order Mycoplasmatales (156a). MORPHOLOGY OF MYCOPLASMAS The mycoplasma organism is distinctive for its small size of 130 to 300 nm and for the lack of a cell wall, the limiting boundary being a plasma 206

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“…1 serologically distinct human Mycoplasma strains and one strain of A. laidlawii A (PG-8). The latter serotype was included because it has been isolated occasionally from humans (8,11).…”

Section: Resultsmentioning

confidence: 99%

Polyacrylamide Gel Identification of Bacterial L-Forms and Mycoplasma Species of Human Origin

Theodore

Tully

Cole

1971

Applied Microbiology

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Polyacrylamide gel electrophoretic patterns of acidified phenol extracts prepared from whole cells can be used for the identification of bacterial L-forms and Mycoplasma species of human origin. Ten human Mycoplasma serotypes and eight L-forms belonging to five different genera were studied. The gel patterns were sufficiently distinct and reproducible that it was possible not only to identify L-forms at the genus level (group with streptococci) and different Mycoplasma serotypes but also to differentiate between the two of them. The parentage of L-forms of Streptobacillus moniliformis Li, Listeria monocytogenes, Streptococcus MG, and Staphylococcus aureus Smith strain was established by relating their gel patterns directly to parent bacteria. It was found that an L-form designated S. moniliformis An (ATCC 14220) was actually an L-form of Proteus. In addition, it was shown electrophoretically that no relationship existed between the Streptococcus MG L-form and M. pneumoniae. The applicability of this method as a diagnostic and taxonomic tool for the differentiation of L-forms and mycoplasmas is discussed.

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Mycoplasma laidlawiiin Human Burns

Cited by 19 publications

References 9 publications

Mycoplasma-Associated Induction of Interferon in Ovine Leukocytes

Mycoplasma-Associated Induction of Interferon in Ovine Leukocytes

Mycoplasmas and cell cultures.

Polyacrylamide Gel Identification of Bacterial L-Forms and Mycoplasma Species of Human Origin

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