Polyacrylamide Gel Identification of Bacterial L-Forms and Mycoplasma Species of Human Origin

Theodore, Theodore S.; Tully, Joseph G.; Cole, Roger M.

doi:10.1128/aem.21.2.272-277.1971

Cited by 11 publications

(4 citation statements)

References 14 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…We found differences between the membrane proteins of the L-form and bacillary form by SDS-polyacrylamide gel electrophoresis which could not be traced to changes in composition of the growth medium. Theodore et al (39) identified the genera of L-forms by comparing their electrophoretic patterns to phenol extracts prepared from whole parental cells. The dissimilarity of band patterns between the bacillus and L-form in our study made identification of the parent-.…”

Section: P-mentioning

confidence: 99%

Characterization of a Stable L-Form ofBacillus subtilis168

1973

View full text Add to dashboard Cite

A stable L-form of Bacillus subtilis 168 (sal-1) has been isolated which grows and divides logarithmically in liquid medium with a generation time of 60 min. This mutant does not synthesize cell wall as evidenced by chemical, biochemical, and morphological analyses. Antibiotics which specifically inhibit cell wall biosynthesis do not affect the growth of the L-form. Significant differences exist between the membrane proteins of the bacillary form and the L-form. The relative profile of membrane proteins varies with the salt concentration of the medium in both the L-form and the bacillary form.

show abstract

Section: P-mentioning

confidence: 99%

Characterization of a Stable L-Form ofBacillus subtilis168

1973

View full text Add to dashboard Cite

show abstract

“…Polyacrylamide gel electrophoresis of the urea-phenol-acetic acid-solubilized membrane proteins reveals 20 to 30 bands (125, 131,148,171). Since not all of the proteins enter the gels, the actual number of membrane proteins may be larger.…”

Section: E F'mentioning

confidence: 99%

Cell biology of the mycoplasmas.

Maniloff¹,

Morowitz²

1972

Bacteriological Reviews

101

View full text Add to dashboard Cite

“…In recent years, reports have been published on the use of PAGE with a great variety of microorganisms, ranging from mycoplasmas (5,6,(27)(28)(29)40) to bacteria and even fungi (Aspergillus fumigatus) (38); the bacteria have included brucellae (20) (43), and Pasteurella haemolytica (42). For the genus Haemophilus, only a few studies of this type have been reported.…”

mentioning

confidence: 99%

Polyacrylamide Gel Electrophoresis of Whole-Cell Proteins of Porcine Strains of Haemophilus

Nicolet

Paroz

Krawinkler

1980

International Journal of Systematic Bacteriology

View full text Add to dashboard Cite

The protein patterns of sodium dodecyl sulfate-solubilized whole cells of porcine strains of Haemophilus were studied by using polyacrylamide gel electrophoresis. The pattern of Haemophilus pleuropneumoniae was very homogenous and was independent of the serological type. At least two different patterns could be distinguished in Haemophilus parasuis, suggesting some heterogeneity in this species. The results were highly reproducible and were not affected by growth conditions. Comparable patterns were obtained after solubilization with sodium taurocholate and sodium carbonate and after ultrasonic treatment, but not after phenol-acetic acid extraction. In addition, we compared porcine strains with human strains (Haemophilus influenzae, Haemophilus parahaemolyticus) and also with some bacteria of the genera Pasteurella, Actinobacillus, Brucella, Moraxella, and Bordetella. The species-specific picture is given mostly by the pattern of a group of proteins with molecular weights just above 68,OOO (Haemophilus) and in the region of molecular weights between 23,000 and 40,000 and between 15,000 and 17,000 (Haemophilus, Pasteurella, Actinobacillus) . These observations suggest the possible use of this method as an aid in studying the taxonomy of these bacteria.The electrophoretic patterns obtained by polyacrylamide gel electrophoresis (PAGE) of cell proteins from bacteria has proved to be useful in taxonomic studies. Since these cell proteins are genetically directed, their patterns tend to express genetic relationships between microorganisms (29).In recent years, reports have been published on the use of PAGE with a great variety of microorganisms, ranging from mycoplasmas (5, 6, 27-29,40) to bacteria and even fungi (Aspergillus fumigatus) (38); the bacteria have included brucellae (20), staphylococci (3), enteric bacteria (26, 30, 32, 33, 44), streptomycetes (7), yersiniae (lo), mycobacteria (8), streptococci (17,18), campylobacters (vibrios) (21), neisseriae (24, 31, 45), Corynebacterium diphtheriae (16), Erysipelothrix rhusiopathiae (43), and Pasteurella haemolytica (42). For the genus Haemophilus, only a few studies of this type have been reported. Neumann and Him (22) found that the protein patterns of strains of Haemophilus paragallinarum, Haemophilus avium, Haemophilus parainfluenzae, and Haemophilus parasuis are quite different, even among strains within the same species, so that the method has been judged to be unreliable for taxonomic purposes.It has been clearly demonstrated that the cytoplasmic membranes and whole-cell proteins of mycoplasmas and also of L-forms of bacteria show highly reproducible and species-specific electrophoretic patterns with PAGE (28,39,40).It is likely in such cases that PAGE can be used as a taxonomic tool. In the case of other bacteria, the majority of authors have observed similarities in the electrophoretic patterns within a genus (20, 32, 41) or within a species (7, 8, 10, 18, 20, 24, 31, 42) but not between genera (20, 32, 41).Despite the rather complex structure of bacterial ce...

show abstract

Polyacrylamide Gel Identification of Bacterial L-Forms and Mycoplasma Species of Human Origin

Cited by 11 publications

References 14 publications

Characterization of a Stable L-Form ofBacillus subtilis168

Characterization of a Stable L-Form ofBacillus subtilis168

Cell biology of the mycoplasmas.

Polyacrylamide Gel Electrophoresis of Whole-Cell Proteins of Porcine Strains of Haemophilus

Contact Info

Product

Resources

About