<i>N</i>‐acetylglucosamine transporter, Ngt1, undergoes sugar‐responsive endosomal trafficking in<i>Candida albicans</i>

Rao, Kongara Hanumantha; Roy, Kasturi; Paul, Soumita; Ghosh, Swagata

doi:10.1111/mmi.14857

Cited by 3 publications

(3 citation statements)

References 88 publications

(146 reference statements)

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“…The exact physiological function of Rsp5 in C. albicans is still not fully understood. To fully comprehend the role and significance of Rsp5 in C. albicans , further research is required [ 111 ].…”

Section: Resultsmentioning

confidence: 99%

“…Rsp5, an E3 ubiquitin ligase, is a central player in temperature sensing, coordination of the heat shock response, and regulation of sugar transporters, all of which significantly impact Candida’s pathogenicity and its ability to adapt to different environments, as indicated by previous studies [ 111 , 140 ]. However, the precise physiological functions of Rsp5 in C. albicans remain incompletely understood and further research is necessary to gain a comprehensive understanding of its role and its significance [ 111 ].…”

Section: Discussionmentioning

confidence: 99%

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Transcriptomic meta-analysis to identify potential antifungal targets in Candida albicans

Hefny,

Ji,

Elsemman

et al. 2024

BMC Microbiol

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Background Candida albicans is a fungal pathogen causing human infections. Here we investigated differential gene expression patterns and functional enrichment in C. albicans strains grown under different conditions. Methods A systematic GEO database search identified 239 “Candida albicans” datasets, of which 14 were selected after rigorous criteria application. Retrieval of raw sequencing data from the ENA database was accompanied by essential metadata extraction from dataset descriptions and original articles. Pre-processing via the tailored nf-core pipeline for C. albicans involved alignment, gene/transcript quantification, and diverse quality control measures. Quality assessment via PCA and DESeq2 identified significant genes (FDR < = 0.05, log2-fold change > = 1 or <= -1), while topGO conducted GO term enrichment analysis. Exclusions were made based on data quality and strain relevance, resulting in the selection of seven datasets from the SC5314 strain background for in-depth investigation. Results The meta-analysis of seven selected studies unveiled a substantial number of genes exhibiting significant up-regulation (24,689) and down-regulation (18,074). These differentially expressed genes were further categorized into 2,497 significantly up-regulated and 2,573 significantly down-regulated Gene Ontology (GO) IDs. GO term enrichment analysis clustered these terms into distinct groups, providing insights into the functional implications. Three target gene lists were compiled based on previous studies, focusing on central metabolism, ion homeostasis, and pathogenicity. Frequency analysis revealed genes with higher occurrence within the identified GO clusters, suggesting their potential as antifungal targets. Notably, the genes TPS2, TPS1, RIM21, PRA1, SAP4, and SAP6 exhibited higher frequencies within the clusters. Through frequency analysis within the GO clusters, several key genes emerged as potential targets for antifungal therapies. These include RSP5, GLC7, SOD2, SOD5, SOD1, SOD6, SOD4, SOD3, and RIM101 which exhibited higher occurrence within the identified clusters. Conclusion This comprehensive study significantly advances our understanding of the dynamic nature of gene expression in C. albicans. The identification of genes with enhanced potential as antifungal drug targets underpins their value for future interventions. The highlighted genes, including TPS2, TPS1, RIM21, PRA1, SAP4, SAP6, RSP5, GLC7, SOD2, SOD5, SOD1, SOD6, SOD4, SOD3, and RIM101, hold promise for the development of targeted antifungal therapies.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Transcriptomic meta-analysis to identify potential antifungal targets in Candida albicans

Hefny,

Ji,

Elsemman

et al. 2024

BMC Microbiol

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“…Several sugar transporters have been found to be internalized to transduce cellular signal intracellularly, such as the N-acetylglucosamine transporter NGT1 in Candida albicans . NGT1 undergoes continuous internalization when exposed to N-acetylglucosamine (GlcNAc) and trafficks from membrane towards vacuoles [ 35 ]. Besides, the cellobiose transporter CRT1 in T. reesei is localized at the cell membranes as well as at the periphery of the nucleus [ 16 ].…”

Section: Discussionmentioning

confidence: 99%

Role of cellulose response transporter-like protein CRT2 in cellulase induction in Trichoderma reesei

Su¹,

Xu²,

Xiang³

2023

Biotechnol Biofuels

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Background Induction of cellulase in cellulolytic fungi Trichoderma reesei is strongly activated by cellulosic carbon sources. The transport of cellulosic inducer and the perception of inducing signal is generally considered as the critical process for cellulase induction, that the inducing signal would be perceived by a sugar transporter/transceptor in T. reesei. Several sugar transporters are coexpressed during the induction stage, but which function they serve and how they work collaboratively are still difficult to elucidate. Results In this study, we found that the constitutive expression of the cellulose response transporter-like protein CRT2 (previously identified as putative lactose permease TRE77517) improves cellulase induction on a cellulose, cellobiose or lactose medium. Functional studies indicate that the membrane-bound CRT2 is not a transporter of cellobiose, lactose or glucose in a yeast system, and it also does not affect cellobiose and lactose utilization in T. reesei. Further study reveals that CRT2 has a slightly similar function to the cellobiose transporter CRT1 in cellulase induction. Overexpression of CRT2 led to upregulation of CRT1 and the key transcription factor XYR1. Moreover, overexpression of CRT2 could partially compensate for the function loss of CRT1 on cellulase induction. Conclusions Our study uncovers the novel function of CRT2 in cellulase induction collaborated with CRT1 and XYR1, possibly as a signal transductor. These results deepen the understanding of the influence of sugar transporters in cellulase production.

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Role of carbon and nitrogen assimilation in Candida albicans survival and virulence

Sahoo,

Rao

2024

The Microbe

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N‐acetylglucosamine transporter, Ngt1, undergoes sugar‐responsive endosomal trafficking inCandida albicans

Cited by 3 publications

References 88 publications

Transcriptomic meta-analysis to identify potential antifungal targets in Candida albicans

Transcriptomic meta-analysis to identify potential antifungal targets in Candida albicans

Role of cellulose response transporter-like protein CRT2 in cellulase induction in Trichoderma reesei

Role of carbon and nitrogen assimilation in Candida albicans survival and virulence

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