N,N‐Dimethylformamide Additions to the Solution for the Electrospinning of Poly(ε‐caprolactone) Nanofibers

Hsu, Chen-Ming; Shivkumar, S.

doi:10.1002/mame.200300224

Cited by 128 publications

(101 citation statements)

References 15 publications

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“…[34][35][36] It has also been reported that the bending instability of polymeric jet is increased when solvents with high dielectric constant are used, resulting in an increased deposition area, the enlargement of the jet path favoring the reduction of fiber size. 37 XRD spectra showed the characteristic (110), (111), (200), and (210) reflections of the orthorhombic crystal structure of PCL, 16,38 as pictured in Figure 4. Thus, electrospinning of PCL seems not to induce significant modifications of its crystal structure.…”

Section: Discussionmentioning

confidence: 99%

Structural characterization and cell response evaluation of electrospun PCL membranes: Micrometric versus submicrometric fibers

Gaudio

Bianco

Folin

et al. 2008

J Biomedical Materials Res

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Electrospinning is a valuable technique to fabricate fibrous scaffolds for tissue engineering. The typical nonwoven architecture allows cell adhesion and proliferation, and supports diffusion of nutrients and waste products. Poly(epsilon-caprolactone) (PCL) electrospun membranes were produced starting from 14% w/v solutions in (a) mixture 1:1 tetrahydrofuran and N,N-dimethylformamide and (b) chloroform. Matrices made up of randomly arranged uniform fibers free of beads were obtained. The average fiber diameters were (a) 0.8 +/- 0.2 microm and (b) 3.6 +/- 0.8 microm. PCL matrices showed the following tensile mechanical properties: tensile modulus (a) 5.0 +/- 0.7 MPa (b) 6.4 +/- 0.2 MPa, yield stress (a) 0.55 +/- 0.06 MPa (b) 0.43 +/- 0.02 MPa, and ultimate tensile stress (a) 1.7 +/- 0.2 MPa and (b) 0.8 +/- 0.1 MPa. The ultimate strain ranged between 300% and 400%. Cytotoxicity of electrospun membranes was continuously evaluated by means of electric cell-substrate impedance sensing technique using human umbilical vein endothelial cells (HUVEC). PCL matrices resulted free of toxic amounts of contaminants and/or process by-products. In vitro studies performed by culturing HUVEC on micrometric and submicrometric fibrous mats showed that both structures supported cell adhesion and spreading. However, cells cultured on the micrometric network showed higher vitality and improved interaction with the polymeric fibers, suggesting an increased ability to promote cell colonization.

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Section: Discussionmentioning

confidence: 99%

Structural characterization and cell response evaluation of electrospun PCL membranes: Micrometric versus submicrometric fibers

Gaudio

Bianco

Folin

et al. 2008

J Biomedical Materials Res

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“…DMF is not a solvent for PCL but it has a high dielectric constant and could enhance electrospinning. Many studies showed that DMF help during the electrospinning and the formation of electrospun fibers [Lee et al, 2003;Hsu and Shivkumar, 2004].…”

Section: Methodsmentioning

confidence: 99%

In vitro Differentiation of Human Cord Blood-Derived Unrestricted Somatic Stem Cells into Hepatocyte-Like Cells on Poly(ε-Caprolactone) Nanofiber Scaffolds

Hashemi¹,

Soleimani²,

Zargarian³

et al. 2008

Cells Tissues Organs

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Tissue engineering of implantable cellular constructs is an emerging cellular therapy for hepatic disease. In this study, we tested the ability of poly(ε-caprolactone) (PCL) nanofiber scaffold to support and maintain hepatic differentiation of human cord blood-derived unrestricted somatic stem cells (USSCs) in vitro. USSCs, self-renewing pluripotent cells, were isolated from human cord blood. The electrospun PCL nanofiber porous scaffold was constructed of uniform, randomly oriented nanofibers. USSCs were seeded onto PCL nanofiber scaffolds, and were induced to differentiate into hepatogenic lineages by culturing with differentiation factors for 6 weeks. RT-PCR analysis of endoderm and hepatic-specific gene expression, immunohistochemical detection of cytokeratin 18 (CK-18), α-fetoprotein, albumin, glycogen storage and indocyanine green uptake confirmed the differentiation of USSCs into endoderm and hepatocyte-like cells. In the present study, we show that hepatocyte-like cells differentiated from USSCs on the PCL nanofiber scaffold can be candidate for tissue engineering and cell therapy of hepatic tissues.

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“…Chloroform is most often applied as solvent for electrospinning PCL but produces rather microfibres instead of nanofibres as the diameters are typically 3 to 5 µm [15,17]. Other relatively highly toxic solvents such as dimethylformamide (DMF), tetrafluoroethylene, methylene chloride, dichloroethane and pyridine have been reported as well [4,6,[18][19][20][21][22]. Some of them allow for nanofibres with diameters in the nanoscale range but in none of the reported systems attention was given to the stability nor the reproducibility of the electrospinning process.…”

Section: Introductionmentioning

confidence: 99%

An alternative solvent system for the steady state electrospinning of polycaprolactone

Schueren

Schoenmaker

Kalaoğlu

et al. 2011

European Polymer Journal

244

139

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Polycaprolactone (PCL) is a biocompatible aliphatic polyester with many possible applications in the medical field. PCL nanofibres, produced by electrospinning, could provide new characteristics that are of interest for these applications. However, a key prerequisite is the ability to obtain bead-free fibres with diameters in the nanoscale range. At present the most commonly used solvent for electrospinning PCL is chloroform, but this only leads to fibres in the microscale range. Therefore various solvent systems were examined in this study.The innovative solvent mixture formic acid/acetic acid was found to allow for nanofibres with a diameter ten times smaller than chloroform. Moreover, steady state conditions could be obtained which thus allow electrospinning in a stable and reproducible way. Further it was noticed that the average fibre diameter decreased with decreasing polymer concentration while the diameter distribution decreased with increasing amount of formic acid. Also the humidity, an often overlooked yet important parameter, was noted to affect both diameter 2 characteristics. Generally it can be concluded that the solvent system formic acid/acetic acid could fill the gap in electrospinning PCL since it is readily able to produce uniform fibres in the nanoscale range.

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N,N‐Dimethylformamide Additions to the Solution for the Electrospinning of Poly(ε‐caprolactone) Nanofibers

Cited by 128 publications

References 15 publications

Structural characterization and cell response evaluation of electrospun PCL membranes: Micrometric versus submicrometric fibers

Structural characterization and cell response evaluation of electrospun PCL membranes: Micrometric versus submicrometric fibers

In vitro Differentiation of Human Cord Blood-Derived Unrestricted Somatic Stem Cells into Hepatocyte-Like Cells on Poly(ε-Caprolactone) Nanofiber Scaffolds

An alternative solvent system for the steady state electrospinning of polycaprolactone

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