N-terminal amino acid sequence of wheat proteins that lack phenylalanine and histidine residues

Abstract

The 24 residues of the N-terminal CNBr peptide from a wheat albumin, that lacks phenylalanine and histidine, have been sequenced. Three of the assignments were made partly by analogy with two other proteins, as evidence is presented that all three proteins are probably identical in this region. Extra evidence for the sequences of the alpha-chymotryptic peptides derived from the N-terminal CNBr peptides of the three proteins, and also for their assembly, has been deposited as Supplementary Publication SUP 50063 (11 pages) at the British Library Lending Division, Boston Spa, Wetherby, West Yorkshire LS23 7BQ, U.K., from whom copies may be obtained on the terms given in Biochem J. (1976) 153, 5. The nature of such evidence is stated in the text of this present communication.

Structural studies of wheat monomeric and dimeric protein inhibitors of α-amylase

“…The recovery of the N-terminal residue (serine) was close to 60 %; such a yield, which is usual for single-chain proteins (Niall, 1973), suggests that this residue was derived from both subunits of inhibitor 0.19. In agreement with the previous reports by Redman (1975Redman ( , 1976, we found that serine is also the N-terminal residue of inhibitor 0.28. We were unable to identify the amino acid phenylthiohydantoin released at the second cycle of the automated Edman procedure with inhibitor 0.19; this product did not give any characteristic peak by g.l.c.…”

“…These two proteins with identical molecular weight not only have similar inhibitory behaviour towards a-amylases from Vol. 173 different origins (Silano et al, 1973) and have homologous amino acid sequences of large peptide fragments (Redman, 1975(Redman, , 1976, but are also characterized by an identical content of disulphide bridges and closely related conformations as monitored by c.d. spectra.…”

“…2. N-Terminal sequence ofinhibitor 0.19 For comparison the N-terminal sequence of inhibitor 0.28, as described by Redman (1976), is also reported. The amino acid sequence of inhibitor 0.19 has been shifted by one position after residue 4 to allow a better overlapping of primary structures of inhibitors 0.19 and 0.28.…”

“…The 12000-mol.wt. inhibitor family consists of five components with a closely related structure as well as a similar inhibitory behaviour towards amylases of different origins (Silano et al, 1973;Redman, 1975Redman, , 1976. The main components of the 12000-mol.wt.…”

Synthesis and Biological Evaluation of an N6-Diethylphospholysyl-Containing Peptide-A Model Compound with a Covalently Attached Metabolite of an Organophosphate Pesticide

“…Compound 3 was used in this study in its nonphosphorylated form only. The basic sequence in 3, Tyr-Lys-Val, occurs naturally in wheat albumin (Redman, 1976), and the preparation and biological evaluation of its N-phosphorylated form will be reported later. Its characteristics are shown in Table 1.…”