<i>Nocardia africana</i>
            sp. nov., a New Pathogen Isolated from Patients with Pulmonary Infections

Hamid, Mohamed E.; Maldonado, Luis A.; Eldin, Ghada S. Sharaf; Mohamed, Maha F.; Saeed, Nageeb S.; Goodfellow, Michael

doi:10.1128/jcm.39.2.625-630.2001

Cited by 75 publications

(42 citation statements)

References 37 publications

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“…beijingensis pertaining to Beijing, the source of the isolate). Hamid et al (2001), Maldonado et al (2000), Yassin et al (2000b) and this study. Aesculin hydrolysis j k k j j j j j j j k j j j k j j j j j j j Nitrate reduction j  j j j k j  j j k j j j  k j j j j j j Urea hydrolysis j j k j j k k k j j k k j j j j j k j j j j Decomposition of (%, w\v) : …”

Section: Description Of Nocardia Beijingensis Sp Novmentioning

confidence: 99%

“…The revised genus encompasses 21 species that form a clade within the evolutionary radiation occupied by the mycolic-acid-containing actinomycetes, that is, by organisms that constitute the suborder Corynebacterineae (Stackebrandt et al, 1997). The taxonomic integrity of most of these species is underpinned by a plethora of molecular systematic and numerical phenetic data Hamid et al, 2001 ;Maldonado et al, 2000). The improved classification of the genus provides an invaluable framework for the recognition of additional species of Nocardia.…”

Section: Introductionmentioning

confidence: 99%

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Nocardia beijingensis sp. nov., a novel isolate from soil.

Wang¹,

Zhang²,

Lu³

et al. 2001

International Journal of Systematic and Evolutionary Microbiology

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The taxonomic status of a soil isolate, provisionally assigned to the genus Nocardia, was established following a polyphasic study. An almost complete sequence of the 16S rDNA of the strain was determined and aligned with available sequences for nocardiae and phylogenetic trees were inferred using four tree-making algorithms. The organism was associated consistently with the type strains of Nocardia brasiliensis and Nocardia farcinica in the neighbour-joining analysis, albeit with low bootstrap values. It was also found to have chemotaxonomic and morphological properties consistent with its assignment to the genus Nocardia. The organism was readily separated from representatives of other Nocardia species by a broad range of phenotypic properties. The name proposed for the novel species is Nocardia beijingensis sp. nov. The type strain is AS4.1521 T (l IFO 16342 T l JCM 10666 T ).

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Section: Description Of Nocardia Beijingensis Sp Novmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Nocardia beijingensis sp. nov., a novel isolate from soil.

Wang¹,

Zhang²,

Lu³

et al. 2001

International Journal of Systematic and Evolutionary Microbiology

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“…While cutaneous disease is still the most common presentation in immunocompetent individuals, such infections also occur in the immunocompromised host. However, pulmonary and disseminated infections are the more common presentations in immunocompromised individuals (16).Molecular methodologies have been instrumental in the recognition or description of several new species of Nocardia which have been recognized as human pathogens (11,(23)(24)(25)(30)(31)(32). For several years we have been using such methodologies to identify Nocardia species isolated from clinical specimens.…”

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confidence: 99%

Nocardia veterana as a Pathogen in North American Patients

et al. 2003

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The molecular methodologies used in our laboratories have allowed us to define a group of Nocardia isolates from clinical samples which resemble the type strain of Nocardia veterana. Three patient isolates and the type strain of N. veterana gave identical and distinctive restriction fragment length polymorphisms (RFLPs) for an amplified portion of the 16S rRNA gene. These three isolates and the N. veterana type strain also gave identical RFLPs for an amplified portion of the 65-kDa heat shock protein gene, but this pattern was identical to that obtained for the Nocardia nova type strain. Sequence analysis of both a 1,359-bp region of the 16S rRNA gene and a 441-bp region of the heat shock protein gene of the patient isolates showed 100% identities with the same regions of the N. veterana type strain. DNA-DNA hybridization of the DNA of one of the patient isolates with the DNA of the N. veterana type strain showed a relative binding ratio of 82%, with 0% divergence, confirming that the isolate was N. veterana. Biochemical and susceptibility testing showed no significant differences among the patient isolates and the N. veterana type strain. Significantly, the results of antimicrobial susceptibility testing obtained for our isolates were similar to those obtained for N. nova, indicating that susceptibility testing alone cannot discriminate between these species. We present two case studies which show that N. veterana is a causative agent of pulmonary disease in immunocompromised patients residing in North America. We also describe difficulties encountered in using 16S rRNA gene sequences alone for discrimination of N. veterana from the related species Nocardia africana and N. nova because of the very high degree of 16S rRNA gene similarity among them.Nocardia species have been implicated as the causes of cutaneous, ocular, pulmonary, and disseminated diseases (16) in both immunocompetent and immunocompromised human hosts. Over the past several years the spectrum of disease caused by Nocardia species has changed due to the increase in the number of immunocompromised patients. While cutaneous disease is still the most common presentation in immunocompetent individuals, such infections also occur in the immunocompromised host. However, pulmonary and disseminated infections are the more common presentations in immunocompromised individuals (16).Molecular methodologies have been instrumental in the recognition or description of several new species of Nocardia which have been recognized as human pathogens (11,(23)(24)(25)(30)(31)(32). For several years we have been using such methodologies to identify Nocardia species isolated from clinical specimens. Our procedure involves PCR amplification of portions of both the 16S rRNA gene and the 65-kDa heat shock protein (HSP) gene and subsequent restriction endonuclease analysis (REA) of the PCR products (6,20). Our experience has shown that in most cases, correct species assignment can be made when the identification obtained by REA of the 16S rRNA gene region is identical to ...

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“…It is also becoming increasingly clear that nocardial species diversity is underestimated in both clinical and non-clinical settings (Gürtler et al, 2001;Hamid et al, 2001;Wang et al, 2001;Yassin et al, 2001;Albuquerque de Barros et al, 2003). It is important to unravel the species richness of nocardiae, especially to determine the roles that members of particular species play in the flow of nutrients and energy in natural habitats.…”

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confidence: 99%

“…Members of these taxa form a distinct phyletic line in the 16S rDNA tree and can be distinguished from one another using a combination of biochemical, chemical and morphological features (Goodfellow et al, 1999). The revised genus Nocardia encompasses 27 species with validly published names at the time of writing; the taxonomic integrity of most of the latter is underpinned by a plethora of genotypic and phenotypic data (Goodfellow et al, 1999;Maldonado et al, 2000;Gürtler et al, 2001;Hamid et al, 2001;Wang et al, 2001;Yassin et al, 2001).…”

mentioning

confidence: 99%