Notch is an alternative splicing gene in brown planthopper, Nilaparvata lugens

Abstract

Much research has assumed that Notch codes one protein. Then the protein will be cleaved into two parts and regenerates a heterodimers receptor to construct Notch signal pathways to regulate development in the past three decades. Here, we show that Notch in brown planthopper is a complex alternatively spliced gene has at least three transcriptional start sites, four exon skips, and 21 transcriptional endpoints that uses these to form variants and codes a series of proteins. When used dsRNAs to suppression different regions of the full‐length variant NlNF resulted in a similar phenotype. Insects were molting after treatment, sensation circles on antennas near to root decayed, bristles on wings shortened, thickened or disappeared, accompanied by thickening veins and blades of fore‐wing apex regions thickened. These results suggested that Notch influenced developmental of sensation circles, bristles, veins, and blades in nymph late periods. This study has deepened our understanding of Notch.

The Expression and Function of Notch Involved in Ovarian Development and Fecundity in Basilepta melanopus

“…The notch gene and its signaling pathway play crucial roles in various developmental processes in insects, including wing formation, embryonic morphogenesis, and regionalization [32][33][34][35]. Studies on the notch gene in the brown planthopper indicate that its expression is greater in adults than in pupae, with elevated expression in the wings and midgut compared to other tissues [33].…”

Notch Signaling in Insect Development: A Simple Pathway with Diverse Functions

“…A recent study in the brown planthopper, Nilaparvata lugens , revealed that the planthopper Notch gene encodes multiple protein isoforms by alternative splicing [ 184 ]. When dsRNA targeting different isoforms were injected into the planthopper nymphs, several Notch variants were found to regulate bristle, vein and wing blade development [ 184 ].…”

“…A recent study in the brown planthopper, Nilaparvata lugens , revealed that the planthopper Notch gene encodes multiple protein isoforms by alternative splicing [ 184 ]. When dsRNA targeting different isoforms were injected into the planthopper nymphs, several Notch variants were found to regulate bristle, vein and wing blade development [ 184 ]. While the expression of Notch pathway genes has been detected in the wing discs of various insect species, their specific functions require further investigation [ 185 , 186 ].…”