<i>NtPDR3</i>, an iron‐deficiency inducible ABC transporter in <i>Nicotiana tabacum</i>

Ducos, Eric; Fraysse, Å. Staffan; Boutry, Marc

doi:10.1016/j.febslet.2005.11.014

Cited by 50 publications

(41 citation statements)

References 34 publications

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“…In contrast to previously reported transcription factors related to plant nutrition, IDEF1 binds specifically to the functionally identified cis-acting element IDE1, which is thought to be functional in a wide range of plant species (8,(16)(17)(18). Transcripts of IDEF1 are constitutively expressed, and its levels do not increase under iron deficiency (Fig.…”

Section: Idef1 As a Key Component In The Regulation Of Iron-deficiencymentioning

confidence: 44%

“…The promoter regions of many iron deficiencyinducible genes in barley, rice, and Arabidopsis possess IDE-like sequences (8,16). In addition, a tobacco ABC transporter gene containing an IDE1-like sequence in its promoter region, Nt-PDR3, was found to be inducible by iron deficiency (18). These facts suggest that gene regulation mechanisms involving IDEs not only are conserved among graminaceous (Strategy II) plants but also are functional in nongraminaceous (Strategy I) plant species.…”

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confidence: 67%

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The transcription factor IDEF1 regulates the response to and tolerance of iron deficiency in plants

Kobayashi

Ogo²,

Itai³

et al. 2007

Proc. Natl. Acad. Sci. U.S.A.

206

219

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Iron is essential for most living organisms and is often the major limiting nutrient for normal growth. Plants induce iron utilization systems under conditions of low iron availability, but the molecular mechanisms of gene regulation under iron deficiency remain largely unknown. We identified the rice transcription factor IDEF1, which specifically binds the iron deficiency-responsive cis-acting element IDE1. IDEF1 belongs to an uncharacterized branch of the plant-specific transcription factor family ABI3/VP1 and exhibits the sequence recognition property of efficiently binding to the CATGC sequence within IDE1. IDEF1 transcripts are constitutively present in rice roots and leaves. Transgenic tobacco plants expressing IDEF1 under the control of the constitutive cauliflower mosaic virus 35S promoter transactivate IDE1-mediated expression only in iron-deficient roots. Transgenic rice plants expressing an introduced IDEF1 exhibit substantial tolerance to iron deficiency in both hydroponic culture and calcareous soil. IDEF1 overexpression leads to the enhanced expression of the iron deficiency-induced transcription factor gene OsIRO2, suggesting the presence of a sequential gene regulatory network. These findings reveal cis element/trans factor interactions that are functionally linked to the iron deficiency response. Manipulation of IDEF1 also provides another approach for producing crops tolerant of iron deficiency to enhance food and biomass production in calcareous soils.ABI3/VP1 ͉ iron deficiency-responsive elements ͉ mugineic acid family phytosiderophores ͉ transgenic rice M ost living organisms require iron for growth and reproduction, and the iron absorbed by plants constitutes a major iron source for animals and humans. Although abundant in mineral soils, iron is sparingly soluble under aerobic conditions at high pH. Consequently, in calcareous soils, which constitute Ϸ30% of the world's cultivated soils, plants often exhibit iron deficiency symptoms manifested as chlorosis (yellowing caused by chlorophyll deficiency), reducing crop yield and quality (1). Higher plants use two major iron uptake strategies under conditions of low iron supply: reduction (Strategy I) and chelation (Strategy II) (2). The Strategy II mechanism is specific to graminaceous plants and is mediated by natural iron chelators, the mugineic acid family phytosiderophores (MAs) (3). Extensive physiological, biochemical, and molecular studies have identified the biosynthetic pathway of MAs and the genes encoding the biosynthetic enzymes (4-9). The expression of these biosynthetic genes is coordinately upregulated in response to iron deficiency (6,8,9), but the molecular mechanisms regulating these iron deficiency-induced genes are poorly understood.Recent studies have demonstrated that a rice iron deficiencyinduced basic helix-loop-helix (bHLH) transcription factor, Os-IRO2, regulates the Strategy II-based iron deficiency response by inducing the related genes (10, 11). The core sequence to which OsIRO2 binds (CACGTGG) is overrepresented in...

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Section: Idef1 As a Key Component In The Regulation Of Iron-deficiencymentioning

confidence: 44%

mentioning

confidence: 67%

The transcription factor IDEF1 regulates the response to and tolerance of iron deficiency in plants

Kobayashi

Ogo²,

Itai³

et al. 2007

Proc. Natl. Acad. Sci. U.S.A.

206

219

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show abstract

“…When expressed in rice, IDE1 and IDE2 induce Fe deficiency-responsive gene expression in roots and leaves (Kobayashi et al 2004). Sequences similar to IDE1 or IDE2 are present in various Fe deficiency-inducible promoters in barley, rice, tobacco, and Arabidopsis (Ducos et al 2005;Kobayashi et al 2003Kobayashi et al , 2005, suggesting that gene regulatory mechanisms involving IDEs are also functional in nongraminaceous plant species. The transcription factors for IDE1 and IDE2 have been characterized; IDE-binding factor 1 (IDEF1) and IDEF2 specifically bind to IDE1 and IDE2, respectively Ogo et al 2008).…”

Section: Transcription Factors Controlling the Fe Homeostasis In Ricementioning

confidence: 99%

Iron Uptake and Loading into Rice Grains

Bashir

Ishimaru

Nishizawa

2010

Rice

115

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“…When introduced into rice, the pair IDE1 and IDE2 is able to induce Fe-deficiency-responsive expression both in roots and leaves [25]. Sequences similar to IDE1 or IDE2 were found in various Fe-deficiency-inducible promoters of barley, rice, tobacco, and Arabidopsis [6,24,26]. This suggests that gene regulation mechanisms involving IDEs are not only conserved among graminaceous (strategy II) plants but are also functional in nongraminaceous (strategy I) plant species.…”

Section: Manipulating Transcription Factors Regulating the Fe Deficiementioning

confidence: 84%