<i>Pantoea</i>sp. Isolated from Tropical Fresh Water Exhibiting<i>N</i>-Acyl Homoserine Lactone Production

Tan, Wen-Si; Yunos, Nina Yusrina Muhamad; Tan, Peng Chiong; Mohamad, Nur Izzati; Adrian, Tan-Guan-Sheng; Yin, Wai‐Fong; Chan, Kok‐Gan

doi:10.1155/2014/828971

Cited by 11 publications

(10 citation statements)

References 30 publications

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“…Figure 4 describes that the optimum pH and temperature were 6.5 (200 mM, potassium phosphate buffer) and 30 °C, respectively. The optimum parameters for CO-oxidation were not far from the growth conditions for the Pantoea species, i.e., typically 25–30 °C and pH 72223.…”

Section: Resultsmentioning

confidence: 96%

Expression and characterization of Pantoea CO dehydrogenase to utilize CO-containing industrial waste gas for expanding the versatility of CO dehydrogenase

Choi

Min

Kim

et al. 2017

Sci Rep

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Although aerobic CO dehydrogenases (CODHs) might be applicable in various fields, their practical applications have been hampered by low activity and no heterologous expression. We, for the first time, could functionally express recombinant PsCODH in E. coli and obtained a highly concentrated recombinant enzyme using an easy and convenient method. Its electron acceptor spectra, optimum conditions (pH 6.5 and 30 °C), and kinetic parameters (kcat of 12.97 s−1, Km of 0.065 mM, and specific activity of 0.86 Umg−1) were examined. Blast furnace gas (BFG) containing 20% CO, which is a waste gas from the steel-making process, was tested as a substrate for PsCODH. Even with BFG, the recombinant PsCODH retained 88.2% and 108.4% activity compared with those of pure CO and 20% CO, respectively. The results provide not only a promising strategy to utilize CO-containing industrial waste gases as cheap, abundant, and renewable resources but also significant information for further studies about cascade reactions producing value-added chemicals via CO2 as an intermediate produced by a CODH-based CO-utilization system, which would ultimately expand the versatility of CODH.

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Section: Resultsmentioning

confidence: 96%

Expression and characterization of Pantoea CO dehydrogenase to utilize CO-containing industrial waste gas for expanding the versatility of CO dehydrogenase

Choi

Min

Kim

et al. 2017

Sci Rep

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“…Most gram negative bacteria expressed genetically QS signaling system, which means that N-acyl-lhomoserine lactones (AHLs) influence many aspect of biofilm formation including motility, adhesion, maturation and dispersion [30,31], and it's widespread in gram-negative bacteria, especially having been described for pseudomonas oryzihabitans. Many genes are responsible for expressing the product of QS and regulating its process in their biofilm bacteria on urinary catheter [32], such as lasR gene in P. oryzihabitans.…”

Section: Molecular Assaymentioning

confidence: 99%

Phenotypic and Genotypic Detection of Biofilm Formation Pseudomonas oryzihabitance and Susceptibility to Antibiotics

Hasson¹

2019

Nano BioMed ENG

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Pseudomonas oryzihabitans is uncommon pathogen, but recently there may be warning of this bacterium to be dangerous, highly virulence, and may cause increase in morbidity and mortality rate, especially if there are multi-drug resistance and biofilm former. Biofilm forming bacteria display resistance to antibiotics, reaching to 1000 time higher than planktonic bacteria. The aim of the current study was to detect Pseudomonas oryzihabitans as biofilm producer and determine the susceptibility to the tested antibiotics. Pseudomonas oryzihabitans was isolated from urine of catheterized patients in Iraqi hospitals and diagnosed as biofilm former phenotypically by congo red and tissue culture plate methods, and genetically by polymerase chain reaction analysis to detect lasR gene (quarm sensing gene) as biofilm indicator and determine its susceptibility to antibiotics by disc diffusion and VITEK AST method. The results revealed that Pseudomonas oryzihabitans was present at 3 isolates; all of them had lasR gene and were resistant to most tested antibiotics at 100%, except that it showed sensitivity rate reaching to 100% to imipenem. From the present study, it was concluded that Pseudomonas oryzihabitans was isolated for the first time as biofilm producer from urinary catheter which possessed lasR gene as biofilm formation indicator, and the multi-drug resistance was considered as a distinctive feature of biofilm formation bacteria.

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“…It was pointed out that Pantoea members had been used to synthesize QS which regulated many phenotypes, such as the production of virulence factor, the aggregation of cells and biofilm formation [30] Pantoea sp. showed QS activity by increasing cell density-dependent exopolysaccharide substance (EPS) synthesis [17,31]. Exopolysaccharide (EPS) was a major component of biofilm bacterial matrix and a powerful virulence factor which protected the bacterial cell from antibiotics action and hosted immune defense [32].…”

Section: Pantoea Spmentioning

confidence: 99%

Occurrence of Biofilm Formation in Serratia fonticola and Pantoea sp. Isolates among Urinary Catheterized Patients

Hasson¹,

Al-Hamadani²,

Al-Azawi³

2018

Nano BioMed ENG

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Serratia fonticola and Pantoea sp. are gram negative bacteria belonging Enterobactericeae, which were considered opportunistic pathogens and resulted in a great number of cases of nosocomial infections with serious problems of multi-drug resistance, leading to increasing morbidity and mortality rate. Recently, they were recorded as biofilm producers. There were only a few studies about the capability of these bacteria of forming biofilm. So our aim was evaluate the occurrence of Serratia fonticola and Pantoea sp. biofilm former phenotypically and genetically with the determination of their abilities to multi-drug resistance. Serratia fonticola and Pantoea sp. isolated from urine catheterized patients who were hospitalized in Iraqi hospitals. They were then examined for detection of biofilm formation phenotypically by congo red and tissue culture plate methods and genetically by detecting SmaI and EsaI genes (quarm sensing genes) in Serratia fonticola and Pantoea sp. respectively by using polymerase chain reaction method and tested for antimicrobial susceptibility by disc diffusion and VITEK2 system according to Clinical and Laboratory Standards Institute (CLSI). Serratia fonticola at 3 and Pantoea sp. at 4 isolates revealed to possess the ability of forming biofilm which contained SmaI and EsaI genes with 100% resistance to most tested antibiotics except imipenem and azithromycin. SmaI and EsaI genes are present in Serratia fonticola and Pantoea sp. respectively, and are responsible for biofilm formation and considered as indicator; biofilm formation is a strong cause of multidrug resistance in bacteria.

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Pantoeasp. Isolated from Tropical Fresh Water ExhibitingN-Acyl Homoserine Lactone Production

Cited by 11 publications

References 30 publications

Expression and characterization of Pantoea CO dehydrogenase to utilize CO-containing industrial waste gas for expanding the versatility of CO dehydrogenase

Expression and characterization of Pantoea CO dehydrogenase to utilize CO-containing industrial waste gas for expanding the versatility of CO dehydrogenase

Phenotypic and Genotypic Detection of Biofilm Formation Pseudomonas oryzihabitance and Susceptibility to Antibiotics

Occurrence of Biofilm Formation in Serratia fonticola and Pantoea sp. Isolates among Urinary Catheterized Patients

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