Pectobacterium carotovorumsubsp.actinidiaesubsp. nov., a new bacterial pathogen causing canker-like symptoms in yellow kiwifruit,Actinidia chinensis

The soft rot Enterobacteriaceae (SRE) Pectobacterium and Dickeya species (formerly classified as pectinolytic Erwinia spp.) cause important diseases on potato and other arable and horticultural crops. They may affect the growing potato plant causing blackleg and are responsible for tuber soft rot in storage thereby reducing yield and quality. Efficient and cost-effective detection and identification methods are essential to investigate the ecology and pathogenesis of the SRE as well as in seed certification programmes. The aim of this review was to collect all existing information on methods available for SRE detection. The review reports on the sampling and preparation of plant material for testing and on over thirty methods to detect, identify and differentiate the soft rot and blackleg causing bacteria to species and subspecies level. These include methods based on biochemical characters, serology, molecular techniques which rely on DNA sequence amplification as well as several less-investigated ones.

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“…Koh et al . ( 2012 ) classified atypical P. carotovorum isolates to new species P. carotovorum subsp. actinidiae subsp.…”

Section: Morphological and Biological Methodsmentioning

confidence: 99%

Detection, identification and differentiation of Pectobacterium and Dickeya species causing potato blackleg and tuber soft rot: a review

Czajkowski

Pérombelon²,

Jafra

et al. 2014

Annals of Applied Biology

191

121

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“…actinidiae has been reported as the causal agent of canker-like symptoms of kiwifruit species Actinidia chinensis in South Korea (1). Canker symptoms caused by P. carotovorum subsp.…”

Section: Genome Announcementmentioning

confidence: 99%

“…actinidiae seems to appear only under conditions of high humidity in the summer season, with the pathogen requiring higher temperatures (~30 to 32°C) to cause disease and not producing the leaf spots characteristic of P. syringae pv. actinidiae (1). We announce here the draft genome sequences of P. carotovorum subsp.…”

Section: Genome Announcementmentioning

confidence: 99%

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Draft Genome Sequences of Pectobacterium carotovorum subsp. actinidiae ICMP 19971 and ICMP 19972, Two Strains Isolated from Actinidia chinensis with Symptoms of Summer Canker in South Korea

et al. 2017

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Pectobacterium carotovorum subsp. actinidiae is the causal agent of summer canker in kiwifruit plants in South Korea. We report here the draft genome sequences of two P. carotovorum subsp. actinidiae strains, ICMP 19971 and ICMP 19972, which were originally isolated from Actinidia chinensis with symptoms of summer canker. These genome sequences will aid in the identification of genetic traits associated with their unusual capacity to cause canker and help understanding of the threat these exotic enterobacteria pose to the New Zealand kiwifruit industry.

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“…betavasculorum is reported almost exclusively on sugar beet [14]; P. aroidearum exhibits with preference for some monocotyledonous plants [15]; P. zantedeschiae strains were isolated from Zantedeschia spp. (Calla lily) [16]; and, P. actinidiae from symptomatic Actinidia chinensis (kiwi fruit) [17]. Recently, some other species have also been described with bacterial strains isolated from surface waters : P. fontis, P. aquaticum and P. versatile [7,18,19].…”

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confidence: 99%

Dickeya dianthicola emerged from a potato culture area exhibiting a wide diversity of pectinolytic pathogens in northern Morocco

Oulghazi¹,

Moumni²,

Khayi³

et al. 2020

Preprint

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Background: Dickeya and Pectobacterium are pectinolytic pathogens that cause damage to many plants including major crops. Emergence of D. solani and re-emergence D. dianthicola were recently observed in potato fields in several continents. The purpose of this work was to describe the species diversity of Dickeya and Pectobacterium collected from potato fields in Northern Morocco, where Dickeya potato pathogens have not been isolated until recently. Results: Along three years, 119 pathogens belonging to Pectobacterium and Dickeya genera were isolated from three potato culture areas and characterized using selective PCR and gapA gene sequencing. Out of them, 19% belonged to P. versatile, 3% to P. carotovorum, 5% to P. polaris, 56% to P. brasiliense, while 17% to D. dianthicola. The taxonomic assignations were confirmed by draft genome analyses of representative isolates belonging to the collected species. D. dianthicola were isolated from a unique area where a wide species diversity of pectinolytic pathogens was also observed. In potato maceration assay, D. dianthicola isolates were more aggressive than Pectobacterium isolates, a feature that should alert stakeholders of a potential threat linked to the emergence of D. dianthicola in this country. By combining Oxford Nanopore Technologies (ONT) and Illumina technologies, the sequence of the complete genome of D. dianthicola LAR.16.03.LID was obtained. A unique circular chromosome of 4,976,211 bp codes for 4,223 predicted proteins. Comparison of the three complete genomes of D. dianthicola strains RNS049, ME23 and LAR.16.03.LID revealed a highly conserved synteny and the occurrence of strain-specific regions associated with the presence of mobile elements. Conclusion: By combining population sampling and genomics, this study highlighted the ecological context from which D. dianthicola emerged in Morocco. Furthermore, the first complete genome of a D. dianthicola strain isolated in Northern Morocco will constitute a reference genome for investigating the dynamics of the emerging D. dianthicola pathogens in this country.

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Pectobacterium carotovorumsubsp.actinidiaesubsp. nov., a new bacterial pathogen causing canker-like symptoms in yellow kiwifruit,Actinidia chinensis

Cited by 50 publications

References 26 publications

Detection, identification and differentiation of Pectobacterium and Dickeya species causing potato blackleg and tuber soft rot: a review

Detection, identification and differentiation of Pectobacterium and Dickeya species causing potato blackleg and tuber soft rot: a review

Draft Genome Sequences of Pectobacterium carotovorum subsp. actinidiae ICMP 19971 and ICMP 19972, Two Strains Isolated from Actinidia chinensis with Symptoms of Summer Canker in South Korea

Dickeya dianthicola emerged from a potato culture area exhibiting a wide diversity of pectinolytic pathogens in northern Morocco

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