PleurotusSpent Mushroom Compost as Green Supplementary Nutrient in Tissue Culture

Abstract

Abstract. This study investigates the effect of Pleurotus spent mushroom compost as green supplementary nutrient in tissue culture. Different concentration of Pleurotus spent mushroom compost in powder (SMC) and activated carbon (SMCAC) were added into Murashige and Skoog media (MS) with 0.1 mg/L 6-benzylaminopurine (BAP). The shoot regeneration of Clinacantus nutans plant such as number of shoots, number of leaves and length of leaves were recorded for four weeks. Collected data was analyzed using one way analysis of variance (ANOVA) and Tukey test through the SPSS Statistics 21 software. The 0.1 g/L SMC recorded the highest leaves numbers. Meanwhile, 0.2 g/L SMC showed highest performance in length of shoot. The SMCAC showed a negative response in number of leaves and shoot as well as length of shoot. In summary, the SMC showed significant performance in number of leave and shoot length but less effective on number of shoot. In contradictory, the SMCAC reported poor performance in shoot regeneration but contribute in absorption of nutrient from environment and storage of the nutrient asfunction of biochar. Therefore, the SMC has a high potential as a green supplementary nutrient for tissue culture. The application of this material has contributes into the green technology via convert waste to product, which is in-line with zero waste concept.

Preliminary Study of <i>Pleurotus ostreatus</i> (Jacq.) P. Kumm. Spent Mushroom Compost as Nutrient Supplement on the Shoot Induction of Fig Tissue Culture

“…Upon all concentrations of SMC tested on the fig culture, 10% of SMC shows slightly higher proliferation of shoot indicates that small amount of SMC could contribute to new formation of shoots at the cutting area. This is in line with the study conducted by Tay et al, 2016 [16] which SMC as an excellent candidate for supplementary nutrient in tissue culture technique but showed a reverse relationship for number of shoot with increase of SMC concentrations. As for length of shoot and number of leaves, higher concentration show higher performance for production of leaves.…”

Evaluation on the effects of P. ostreatus spent mushroom compost and BAP hormone towards C. nutans in vitro culture

“…9 shows that T3c was the most optimum media to be applied for high leaves formation. In [25] have done a slightly similar research on C. nutans by using SMC only in the growth media. The result found that SMC only did give significant high number of leaves at average 7.…”

<i>Zea mays</i> stem agricultural waste for <i>in-vitro</i> shoot propagation of <i>Clinacantus nutans</i>

“…Consequently, a fast propagation in-vitro tissue culture technique is considered by researchers in order to produce enormous high quality and quantity of plantlets in short time. However, this technique is high in cost due to synthetic hormone and additional chemicals [2]. With this regards, an alternative nutrient source is addressed to enhance shoot propagation and cost reduction.…”