<i>Populus euphratica</i> APYRASE2 Enhances Cold Tolerance by Modulating Vesicular Trafficking and Extracellular ATP in Arabidopsis Plants

Deng, Shumin; Sun, Jian; Zhao, Rui; Ding, Mingquan; Zhang, Yinan; Yuanling, Sun; Wang, Wei; Tan, Yeqing; Liu, Dandan; Ma, Xujun; Hou, Peichen; Wang, Meijuan; Lu, Cunfu; Shen, Xin; Chen, Shao‐Liang

doi:10.1104/pp.15.00581

Cited by 87 publications

(87 citation statements)

References 95 publications

(153 reference statements)

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“…4). This suggested that ATP hydrolysis in RIL82 plants were adversely inhibited by cold stress, and thus the lower GSH synthesis because of the higher unavailable ATP (Puhakainen et al 1999;Mendoza et al 2000;Deng et al 2015;Muzi et al 2016). This hypothesis was confirmed by the present results that the PARP inhibitor (3-ab) only enhanced the cold tolerance and GSH content in ZZ39 plants under cold stress ( Fig.…”

Section: The Role Of Atp Hydrolysis In Gsh Synthesis In Rice Plants Usupporting

confidence: 84%

ATP Hydrolysis Determines Cold Tolerance by Regulating Available Energy for Glutathione Synthesis in Rice Seedling Plants

Jiang

et al. 2020

Rice

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Background: Glutathione (GSH) is important for plants to resist abiotic stress, and a large amount of energy is required in the process. However, it is not clear how the energy status affects the accumulation of GSH in plants under cold stress. Results: Two rice pure lines, Zhongzao39 (ZZ39) and its recombinant inbred line 82 (RIL82) were subjected to cold stress for 48 h. Under cold stress, RIL82 suffered more damages than ZZ39 plants, in which higher increases in APX activity and GSH content were showed in the latter than the former compared with their respective controls. This indicated that GSH was mainly responsible for the different cold tolerance between these two rice plants. Interestingly, under cold stress, greater increases in contents of carbohydrate, NAD(H), NADP(H) and ATP as well as the expression levels of GSH1 and GSH2 were showed in RIL82 than ZZ39 plants. In contrast, ATPase content in RIL82 plants was adversely inhibited by cold stress while it increased significantly in ZZ39 plants. This indicated that cold stress reduced the accumulation of GSH in RIL82 plants mainly due to the inhibition on ATP hydrolysis rather than energy deficit. Conclusion: We inferred that the energy status determined by ATP hydrolysis involved in regulating the cold tolerance of plants by controlling GSH synthesis.

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Section: The Role Of Atp Hydrolysis In Gsh Synthesis In Rice Plants Usupporting

confidence: 84%

ATP Hydrolysis Determines Cold Tolerance by Regulating Available Energy for Glutathione Synthesis in Rice Seedling Plants

Jiang

et al. 2020

Rice

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“…Extracellular ATP levels were monitored with the Enlighten ATP assay system bioluminescence kit (Promega, Madison, WI, United States; Sun et al, 2012a ; Deng et al, 2015 ). Briefly, G. uralensis roots were exposed to 0 or 100 mM NaCl at room temperature.…”

Section: Methodsmentioning

confidence: 99%

“…eATP was measured in an assay with luciferin-luciferase Turner Designs Modulus TM Microplate Multimode Reader (Promega Corp., Madison, WI, United States). The eATP levels were calculated, based on a standard curve created by measuring a linear range (0.01–100 nM) of standard eATP concentrations ( Sun et al, 2012a ; Deng et al, 2015 ).…”

Section: Methodsmentioning

confidence: 99%

Salt-Sensitive Signaling Networks in the Mediation of K+/Na+ Homeostasis Gene Expression in Glycyrrhiza uralensis Roots

et al. 2017

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We investigated the effects of salt-sensitive signaling molecules on ionic fluxes and gene expression related to K+/Na+ homeostasis in a perennial herb, Glycyrrhiza uralensis, during short-term NaCl stress (100 mM, 24 h). Salt treatment caused more pronounced Na+ accumulation in root cells than in leaf cells. Na+ ions were mostly compartmentalized in vacuoles. Roots exposed to NaCl showed increased levels of extracellular ATP (eATP), cytosolic Ca2+, H2O2, and NO. Steady-state flux recordings revealed that these salt-sensitive signaling molecules enhanced NaCl-responsive Na+ efflux, due to the activated Na+/H+ antiport system in the plasma membrane (PM). Moreover, salt-elicited K+ efflux, which was mediated by depolarization-activated cation channels, was reduced with the addition of Ca2+, H2O2, NO, and eATP. The salt-adaptive effects of these molecules (Na+ extrusion and K+ maintenance) were reduced by pharmacological agents, including LaCl3 (a PM Ca2+ channel inhibitor), DMTU (a reactive oxygen species scavenger), cPTIO (an NO scavenger), or PPADS (an antagonist of animal PM purine P2 receptors). RT-qPCR data showed that the activation of the PM Na+/H+ antiport system in salinized roots most likely resulted from the upregulation of two genes, GuSOS1 and GuAHA, which encoded the PM Na+/H+ antiporter, salt overly sensitive 1 (SOS1), and H+-ATPase, respectively. Clear interactions occurred between these salt-sensitive agonists to accelerate transcription of salt-responsive signaling pathway genes in G. uralensis roots. For example, Ca2+, H2O2, NO, and eATP promoted transcription of GuSOS3 (salt overly sensitive 3) and/or GuCIPK (CBL-interacting protein kinase) to activate the predominant Ca2+-SOS signaling pathway in salinized liquorice roots. eATP, a novel player in the salt response of G. uralensis, increased the transcription of GuSOS3, GuCIPK, GuRbohD (respiratory burst oxidase homolog protein D), GuNIR (nitrate reductase), GuMAPK3, and GuMAPK6 (the mitogen-activated protein kinases 3 and 6). Moreover, GuMAPK3 and GuMAPK6 expression levels were enhanced by H2O2 in NaCl-stressed G. uralensis roots. Our results indicated that eATP triggered downstream components and interacted with Ca2+, H2O2, and NO signaling to maintain K+/Na+ homeostasis. We propose that a multiple signaling network regulated K+/Na+ homeostasis in NaCl-stressed G. uralensis roots.

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“…Most recently, eATP was reported to be involved in cold tolerance of P. euphratica (Deng et al 2015). Cold stress stimulated the release of ATP.…”

Section: Stress Responsesmentioning

confidence: 99%

Extracellular ATP: An Essential Apoplastic Messenger in Plants

Liu

Shang

2016

Progress in Botany

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Populus euphratica APYRASE2 Enhances Cold Tolerance by Modulating Vesicular Trafficking and Extracellular ATP in Arabidopsis Plants

Cited by 87 publications

References 95 publications

ATP Hydrolysis Determines Cold Tolerance by Regulating Available Energy for Glutathione Synthesis in Rice Seedling Plants

ATP Hydrolysis Determines Cold Tolerance by Regulating Available Energy for Glutathione Synthesis in Rice Seedling Plants

Salt-Sensitive Signaling Networks in the Mediation of K+/Na+ Homeostasis Gene Expression in Glycyrrhiza uralensis Roots

Extracellular ATP: An Essential Apoplastic Messenger in Plants

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