Porphyromonas gingivalisaffects host collagen degradation by affecting expression, activation, and inhibition of matrix metalloproteinases

Abstract

Porphyromonas gingivalis increased the collagen degrading ability of HGFs, in part, by increasing MMP activation and by lowering the TIMP-1 protein level, as well as by affecting the mRNA expression of multiple MMPs and TIMPs.

Effect of Porphyromonas gingivalis PrtC on cytokine expression in ECV304 endothelial cells and its level in subgingival plaques from patients with chronic periodontitis

“…Much attention has been given to proteinase produced by host cells. MMP, enzymes secreted by endothelial cells, macrophages, and fibroblasts, are known to be involved in tissue remodeling, organogenesis, angiogenesis, wound repair, and inflammatory cellular infiltration by degrading extracellular matrix [16,30] . At the inflammatory site, inflammatory cells are mobilized and MMP are produced as pro-enzymes and activated by various stimulatory factors [30] .…”

“…At the inflammatory site, inflammatory cells are mobilized and MMP are produced as pro-enzymes and activated by various stimulatory factors [30] . Recent studies demonstrated that MMP expression was upregulated by pro-inflammatory mediators including IL-1 and TNF-α [16] . Taken together, these findings suggest that although the prtC gene product may not be a true bacterial collagenase, it could have a strong effect on inducing host cells to secret inflammatory cytokines and further enhance the collagenase activity of host cells such as MMP, which lead to the destruction of periodontal connective tissue.…”

“…Proteinase produced by some bacteria could have a direct effect on inducing inflammation besides its specific enzyme activity [13] and may work in combination with true bacterial or host collagenases [14,15] . Recent findings indicate that P gingivalis induces host collagen degradation by affecting expression, activation, and inhibition of matrix metalloproteinases (MMP) produced by host cells [8,16] . MMP is thought to be transcriptionally upregulated by proinflammatory mediators, such as interleukin (IL)-1 and TNF-α, as well as post-translationally activated by proteases from P gingivalis [16] .…”

“…Recent findings indicate that P gingivalis induces host collagen degradation by affecting expression, activation, and inhibition of matrix metalloproteinases (MMP) produced by host cells [8,16] . MMP is thought to be transcriptionally upregulated by proinflammatory mediators, such as interleukin (IL)-1 and TNF-α, as well as post-translationally activated by proteases from P gingivalis [16] . However, studies on the prtC gene product of P gingivalis mainly concentrate on its collagenolytic activity.…”

In Vitro Effects of Nicotine, Cigarette Smoke Condensate, and Porphyromonas gingivalis on Monocyte Chemoattractant Protein-1 Expression from Cultured Human Gingival Fibroblasts

“…Cell viability and sublethal cytotoxic effects on the cells were determined according to previous studies [11,12]. A control group comprised HGFs without treatment.…”