<i>Pseudomonas aeruginosa</i> strain RW41 mineralizes 4‐chlorobenzenesulfonate, the major polar by‐product from DDT manufacturing

Blasco, Rafael; Ramos, Juan L.; Wittich, Rolf-Michael

doi:10.1111/j.1462-2920.2008.01575.x

Cited by 4 publications

(1 citation statement)

References 37 publications

(45 reference statements)

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“…Numerous bacteria have been discovered that can degrade DDT (Stenerse 1965;Nadeau et al 1994;Bidlan and Manonmani 2002;Ahuja and Kumar 2003;Kamanavalli and Ninnekar 2004;Blasco et al 2008;Wang et al 2010). However, the enzymes responsible for the early steps in this degradation have yet to be identified.…”

Section: Bacterial Enzymesmentioning

confidence: 99%

The evolution of new enzyme function: lessons from xenobiotic metabolizing bacteria versus insecticide‐resistant insects

Russell

Scott

Jackson

et al. 2011

Evolutionary Applications

124

100

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Here, we compare the evolutionary routes by which bacteria and insects have evolved enzymatic processes for the degradation of four classes of synthetic chemical insecticide. For insects, the selective advantage of such degradative activities is survival on exposure to the insecticide, whereas for the bacteria the advantage is simply a matter of access to additional sources of nutrients. Nevertheless, bacteria have evolved highly efficient enzymes from a wide variety of enzyme families, whereas insects have relied upon generalist esterase-, cytochrome P450- and glutathione-S-transferase-dependent detoxification systems. Moreover, the mutant insect enzymes are less efficient kinetically and less diverged in sequence from their putative ancestors than their bacterial counterparts. This presumably reflects several advantages that bacteria have over insects in the acquisition of new enzymatic functions, such as a broad biochemical repertoire from which new functions can be evolved, large population sizes, high effective mutation rates, very short generation times and access to genetic diversity through horizontal gene transfer. Both the insect and bacterial systems support recent theory proposing that new biochemical functions often evolve from ‘promiscuous’ activities in existing enzymes, with subsequent mutations then enhancing those activities. Study of the insect enzymes will help in resistance management, while the bacterial enzymes are potential bioremediants of insecticide residues in a range of contaminated environments.

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