<i>Pseudomonas montielli</i>-A Potent Chemotherapeutic Asparaginase producing organism Isolated from Rhizosphere Soil

Subhashini, A.; Vani, G. Sangeetha

doi:10.5958/0974-360x.2020.00178.x

Cited by 2 publications

(1 citation statement)

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“…It can also be isolated from soil microflora. Pseudomonas aeruginosa is one such organism from which L Asparaginase can be isolated (Subhashini and Vani, 2020). Long term usage of this enzyme results in side effects particularly in remission induction phase like hypersensitivity, low blood pressure, stomach cramps, diabetes, drug induced liver disease, pancreatitis etc (Wang et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

L-Asparaginase – A prospective anticancer enzyme on MCF-7 cell lines extracted from soil Microflora

Subhashini¹,

Vani²

2023

EEC

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This study aims at investigating the soil bacteria for L asparaginase enzyme production and its anti-cancer effect on MCF 7 cell line. In this study, 30 soil samples were screened for bacterial producing L asparaginase enzyme. The soil samples were collected from various places in Ethiraj College, Chennai. Isolation was done using spread plate technique, biochemical and microscopic characterization for the potential isolates revealed the bacterial genera as Bacillus, Escherichia and Pseudomonas species. Of the thirty samples collected, six isolates produced pronounced L asparaginase enzyme. These samples were further subjected to crude enzyme extraction and estimation. The isolates S2 and Ni5 showed maximum production of enzyme with 1090 units/ml of activity. Dialysis was carried out to concentrate the enzyme and molecular characterization was performed using SDS PAGE to separate the proteins according to the molecular weight which was found to be approximately 100 to 116 kDa. Anti-cancer activity was evaluated on MCF 7 tumour cell line. The cell viability was dose dependent and IC50 of S2 was 62.5 μg and Ni5 31.2 μg respectively.

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Section: Introductionmentioning

confidence: 99%

L-Asparaginase – A prospective anticancer enzyme on MCF-7 cell lines extracted from soil Microflora

Subhashini¹,

Vani²

2023

EEC

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Synergistic Combinatorial effect of L-asparaginase and Trastuzumab against HER2+ breast cancer cells

Chand

Sharma

Prasad

2022

RJPT

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Multi-targeted drug combinations which selectively inhibit the proliferation of cancer cells are required for effective anticancer treatment. The current anti-HER2 positive breast cancer therapy involves Trastuzumab and doxorubicin drug combination which produces toxic side effects in clinical settings including high cardiotoxicity. In this study, anticancer activity of single drug as well as the drug combination effect of L-asparaginase (Celginase) and Trastuzumab (Herceptin) was studied on HER2 positive breast cancer (SKBR3) cells. Inhibition of cell proliferation assay based on fluorescence readout was studied to estimate the anticancer effect of the drugs. HUVEC cells were used as negative control cells. Individually as a single drug, Trastuzumab (Herceptin) and L-asparaginase (Celginase) showed anticancer activity against SKBR3 cells with IC50 value of 0.031ng/ml and 1.168µg/ml respectively. The drug combination interaction of Trastuzumab and L-asparaginase resulted in combination index of less than 1(CI < 1) showing their synergistic effect against SKBR3 cells. No cytotoxic effect was observed in control HUVEC cells. The results suggested that the antitumor activity of Trastuzumab and L-asparaginase against HER2 positive breast cancer was found specific to HER2 positive cancer (SKBR3) cells. This synergistic drug interaction of L-asparaginase and Trastuzumab could be further explored to as an alternative to current drug combination therapy against the HER2 positive breast cancer.

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Pseudomonas montielli-A Potent Chemotherapeutic Asparaginase producing organism Isolated from Rhizosphere Soil

Cited by 2 publications

References 0 publications

L-Asparaginase – A prospective anticancer enzyme on MCF-7 cell lines extracted from soil Microflora

L-Asparaginase – A prospective anticancer enzyme on MCF-7 cell lines extracted from soil Microflora

Synergistic Combinatorial effect of L-asparaginase and Trastuzumab against HER2+ breast cancer cells

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