QDtbn1, an F‐box gene affecting maize tassel branch number by a dominant model

Qin, Xiner; Tian, Shike; Zhang, Wenliang; Dong, Xue; Ma, Chengxin; Wang, Yi; Yan, Jianbing; Yue, Bing

doi:10.1111/pbi.13540

Cited by 14 publications

(9 citation statements)

References 57 publications

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“…By integrating the results of genetic mapping conducted over the past 20 years, we constructed a consensus physical map for tassel traits and extracted 97 HSIs ( Figure 4 ), which lays the foundation for subsequent candidate gene cloning, and provides valuable targets for MAS in improving maize yield via tassel morphology modification. Application of the quantitative genetic variations in Q Dtbn1 locus for tassel-trait improvement has been reported [ 89 ]. However, GWAS studies aimed at the discovery of more tassel-trait QTNs will still be the main task in the future.…”

Section: Discussionmentioning

confidence: 99%

“…However, there has been some progress in the application of the quantitative genetic variations in maize tassel-trait improvement recently. For example, one QTN ( Q Dtbn1 ) for TBN was detected by GWAS located in the 5′-upstream of an F-box/kelch-repeat protein coding gene; the TBN was significantly reduced in F-box gene over-expression and the hybrid lines, indicating that Q Dtbn1 negatively influences TBN via a dominant model, and making Q Dtbn1 particularly valuable in maize hybrid breeding [ 89 ].…”

Section: Application Of the Quantitative Genetic Variation Regulating...mentioning

confidence: 99%

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Genetic Structure and Molecular Mechanisms Underlying the Formation of Tassel, Anther, and Pollen in the Male Inflorescence of Maize (Zea mays L.)

Bao

Wei

et al. 2022

Cells

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Maize tassel is the male reproductive organ which is located at the plant’s apex; both its morphological structure and fertility have a profound impact on maize grain yield. More than 40 functional genes regulating the complex tassel traits have been cloned up to now. However, the detailed molecular mechanisms underlying the whole process, from male inflorescence meristem initiation to tassel morphogenesis, are seldom discussed. Here, we summarize the male inflorescence developmental genes and construct a molecular regulatory network to further reveal the molecular mechanisms underlying tassel-trait formation in maize. Meanwhile, as one of the most frequently studied quantitative traits, hundreds of quantitative trait loci (QTLs) and thousands of quantitative trait nucleotides (QTNs) related to tassel morphology have been identified so far. To reveal the genetic structure of tassel traits, we constructed a consensus physical map for tassel traits by summarizing the genetic studies conducted over the past 20 years, and identified 97 hotspot intervals (HSIs) that can be repeatedly mapped in different labs, which will be helpful for marker-assisted selection (MAS) in improving maize yield as well as for providing theoretical guidance in the subsequent identification of the functional genes modulating tassel morphology. In addition, maize is one of the most successful crops in utilizing heterosis; mining of the genic male sterility (GMS) genes is crucial in developing biotechnology-based male-sterility (BMS) systems for seed production and hybrid breeding. In maize, more than 30 GMS genes have been isolated and characterized, and at least 15 GMS genes have been promptly validated by CRISPR/Cas9 mutagenesis within the past two years. We thus summarize the maize GMS genes and further update the molecular regulatory networks underlying male fertility in maize. Taken together, the identified HSIs, genes and molecular mechanisms underlying tassel morphological structure and male fertility are useful for guiding the subsequent cloning of functional genes and for molecular design breeding in maize. Finally, the strategies concerning efficient and rapid isolation of genes controlling tassel morphological structure and male fertility and their application in maize molecular breeding are also discussed.

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Section: Discussionmentioning

confidence: 99%

Section: Application Of the Quantitative Genetic Variation Regulating...mentioning

confidence: 99%

Genetic Structure and Molecular Mechanisms Underlying the Formation of Tassel, Anther, and Pollen in the Male Inflorescence of Maize (Zea mays L.)

Bao

Wei

et al. 2022

Cells

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“…Other authors [ 44 ] have reported that BAD1 is a TCP class II gene that functions to promote cell proliferation in a lateral organ, the pulvinus, and affects the inflorescence architecture by influencing lateral branch emergence angle. According to Qin et al [ 45 ], tassel branch number (TBN) is an important agronomic trait that directly contributes to grain yield in maize ( Zea mays L.), and the identification of genes precisely regulating TBN in the parental lines was important for maize hybrid breeding. The authors concluded that the SCF (Skp1/Cul1/F-box protein/Roc1) complex and the ABA signaling pathway might be involved in TBN modulation in maize.…”

Section: Discussionmentioning

confidence: 99%

DArTseq-Based High-Throughput SilicoDArT and SNP Markers Applied for Association Mapping of Genes Related to Maize Morphology

Tomkowiak

Bocianowski

Spychała

et al. 2021

IJMS

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Today, agricultural productivity is essential to meet the needs of a growing population, and is also a key tool in coping with climate change. Innovative plant breeding technologies such as molecular markers, phenotyping, genotyping, the CRISPR/Cas method and next-generation sequencing can help agriculture meet the challenges of the 21st century more effectively. Therefore, the aim of the research was to identify single-nucleotide polymorphisms (SNPs) and SilicoDArT markers related to select morphological features determining the yield in maize. The plant material consisted of ninety-four inbred lines of maize of various origins. These lines were phenotyped under field conditions. A total of 14 morphological features was analyzed. The DArTseq method was chosen for genotyping because this technique reduces the complexity of the genome by restriction enzyme digestion. Subsequently, short fragment sequencing was used. The choice of a combination of restrictases allowed the isolation of highly informative low copy fragments of the genome. Thanks to this method, 90% of the obtained DArTseq markers are complementary to the unique sequences of the genome. All the observed features were normally distributed. Analysis of variance indicated that the main effect of lines was statistically significant (p < 0.001) for all 14 traits of study. Thanks to the DArTseq analysis with the use of next-generation sequencing (NGS) in the studied plant material, it was possible to identify 49,911 polymorphisms, of which 33,452 are SilicoDArT markers and the remaining 16,459 are SNP markers. Among those mentioned, two markers associated with four analyzed traits deserved special attention: SNP (4578734) and SilicoDArT (4778900). SNP marker 4578734 was associated with the following features: anthocyanin coloration of cob glumes, number of days from sowing to anthesis, number of days from sowing to silk emergence and anthocyanin coloration of internodes. SilicoDArT marker 4778900 was associated with the following features: number of days from sowing to anthesis, number of days from sowing to silk emergence, tassel: angle between the axis and lateral branches and plant height. Sequences with a length of 71 bp were used for physical mapping. The BLAST and EnsemblPlants databases were searched against the maize genome to identify the positions of both markers. Marker 4578734 was localized on chromosome 7, the closest gene was Zm00001d022467, approximately 55 Kb apart, encoding anthocyanidin 3-O-glucosyltransferase. Marker 4778900 was located on chromosome 7, at a distance of 45 Kb from the gene Zm00001d045261 encoding starch synthase I. The latter observation indicated that these flanking SilicoDArT and SNP markers were not in a state of linkage disequilibrium.

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“…However, the establishment of high-density genetic maps of single-nucleotide polymorphism (SNP) markers and genome-wide association study (GWAS) analysis of natural populations provide powerful tools for the fine mapping and analysis of quantitative traits. For instance, Qin employed Mo17 as a test inbred line to conduct whole-genome association analysis and identified the tassel branch-related gene Q Dtbn1 ( Qin et al., 2021 ). Using a similar strategy, Wu identified 63 QTLs distributed on 10 chromosomes, primarily concentrated on chromosomes 1, 2, and 7, that are associated with tassel branches ( Wu et al., 2016 ).…”

Section: Introductionmentioning

confidence: 99%

Identification of QTLs and their candidate genes for the number of maize tassel branches in F2 from two higher generation sister lines using QTL mapping and RNA-seq analysis

Ruidong,

Shijin,

Yuwei

et al. 2023

Front. Plant Sci.

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Tassel branch number is an important agronomic trait that is closely associated with maize kernels and yield. The regulation of genes associated with tassel branch development can provide a theoretical basis for analyzing tassel branch growth and improving maize yield. In this study. we used two high-generation sister maize lines, PCU (unbranched) and PCM (multiple-branched), to construct an F2 population comprising 190 individuals, which were genotyped and mapped using the Maize6H-60K single-nucleotide polymorphism array. Candidate genes associated with tassel development were subsequently identified by analyzing samples collected at three stages of tassel growth via RNA-seq. A total of 13 quantitative trait loci (QTLs) and 22 quantitative trait nucleotides (QTNs) associated with tassel branch number (TBN) were identified, among which, two major QTLs, qTBN6.06-1 and qTBN6.06-2, on chromosome 6 were identified in two progeny populations, accounting for 15.07% to 37.64% of the phenotypic variation. Moreover, we identified 613 genes that were differentially expressed between PCU and PCM, which, according to Kyoto Encyclopedia of Genes and Genomes enrichment analysis, were enriched in amino acid metabolism and plant signal transduction pathways. Additionally, we established that the phytohormone content of Stage I tassels and the levels of indole-3-acetic acid (IAA) and IAA-glucose were higher in PCU than in PCM plants, whereas contrastingly, the levels of 5-deoxymonopolyl alcohol in PCM were higher than those in PCU. On the basis of these findings, we speculate that differences in TBN may be related to hormone content. Collectively, by combining QTL mapping and RNA-seq analysis, we identified five candidate genes associated with TBN. This study provides theoretical insights into the mechanism of tassel branch development in maize.

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Q^Dtbn1, an F‐box gene affecting maize tassel branch number by a dominant model

Cited by 14 publications

References 57 publications

Genetic Structure and Molecular Mechanisms Underlying the Formation of Tassel, Anther, and Pollen in the Male Inflorescence of Maize (Zea mays L.)

Genetic Structure and Molecular Mechanisms Underlying the Formation of Tassel, Anther, and Pollen in the Male Inflorescence of Maize (Zea mays L.)

DArTseq-Based High-Throughput SilicoDArT and SNP Markers Applied for Association Mapping of Genes Related to Maize Morphology

Identification of QTLs and their candidate genes for the number of maize tassel branches in F2 from two higher generation sister lines using QTL mapping and RNA-seq analysis

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