<i>Retracted:</i>Effects of miR‐145‐5p through<scp>NRAS</scp>on the cell proliferation, apoptosis, migration, and invasion in melanoma by inhibiting<scp>MAPK</scp>and<scp>PI</scp>3K/<scp>AKT</scp>pathways

Liu, Sha; Gao, Guozhen; Ding, Yan; Chen, Xiangjun; Yao, Xingwei; Guo, Shuzhong; Li, Gui-Rong; Zhao, Yu

doi:10.1002/cam4.1030

Cited by 73 publications

(74 citation statements)

References 45 publications

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“…In the present study, they confirmed that the MAPK signaling pathway in T24 cells were synergistically repressed by the cotreatment with miR‐143/‐145 . Similarly, through targeting NRAS, miR‐145‐5p could suppress cell functions by inhibiting MAPK pathways . These were the same exploration as us that miR‐145‐5p inhibited MYD88 and related proteins in MAPK signaling pathway.…”

Section: Discussionsupporting

confidence: 86%

Circular RNA CEP128 promotes bladder cancer progression by regulating Mir‐145‐5p/Myd88 via MAPK signaling pathway

Sun

Zhao

Chen

et al. 2019

Intl Journal of Cancer

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The present experiment was designed for exploring the regulatory mechanism of circ‐CEP128/miR‐145‐5p/MYD88 axis in bladder cancer. MiRNAs and circRNAs expression data were derived from Gene Expression Omnibus database with bladder tumor tissues and paracarcinoma tissue samples. Differentially expressed genes in tumor were analyzed via R software. Interaction network of differently expressed miRNAs and differently expressed mRNA was established by means of Cytoscape software. CircCEP128 and miR‐145‐5p expression levels were determined using qRT‐PCR. The expression of MAPK signaling‐related proteins MYD88, p38, ERK and JNK was examined by western blot. The relationship between circCEP128 and miR‐145‐5p was validated using RNA immunoprecipitation. The level of cell propagation and migration was determined by CCK8 and wound healing assay, 5‐bromo‐2′‐deoxyuridine assay and migration assay. Cell apoptosis rate and cell cycle were detected via flow cytometry. Tumor xenograft assay was implemented to investigate the function of circCEP128 in vivo. CircCEP128 and MYD88 were overexpressed in bladder cancer based on microarray analysis and miR‐145‐5p was a potential targeting factor in bladder cancer. CircCEP128 targeted miR‐145‐5p and miR‐145‐5p targeted MYD88. Expression of miR‐145‐5p was decreased in cancer samples. Knockdown of circCEP128 induced the inhibition of cell viability and mobility and cell cycle arrest. Overexpression of miR‐145‐5p or knockdown of circCEP128 promoted MAKP signaling pathway and related proteins expression. In addition, knockdown of circCEP128 suppressed the growth of bladder cancer tumor tissues in vivo. Overexpression of circCEP128 promoted bladder cancer progression through modulating miR‐145‐5p and MYD88 via MAKP signaling pathway.

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Section: Discussionsupporting

confidence: 86%

Circular RNA CEP128 promotes bladder cancer progression by regulating Mir‐145‐5p/Myd88 via MAPK signaling pathway

Sun

Zhao

Chen

et al. 2019

Intl Journal of Cancer

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“…[29][30][31][32] LPS increases the secretion of pro-inflammatory cytokines in fibroblast-like synoviocytes, which leads to the activation of the PI3K/ AKT and MAPK/mTOR pathways, thereby inducing apoptotic and inflammatory injury in these cells. [34][35][36][37] We demonstrated that miR-145 could partially eliminate the LPS-induced activation of PI3K/AKT and MAPK/ mTOR pathways in MH7A cells, implying that the protective activities of miR-145 might be realized by blocking the PI3K/AKT and MAPK/mTOR pathways. Protein expressions of core components of (A), PI3K/AKT, and (B), MAPK pathways were evaluated, after MH7A cells were transfected with miR-145 mimic or mimic-control, and subsequently treated with 5 μg/mL of LPS for 5 hours.…”

Section: Discussionmentioning

confidence: 81%

Retracted: miR‐145 eliminates lipopolysaccharides‐induced inflammatory injury in human fibroblast‐like synoviocyte MH7A cells

Zhong

Yang

et al. 2018

J of Cellular Biochemistry

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Recently, it has been accepted that miR-based therapy may be beneficial for rheumatoid arthritis (RA). This study aimed to evaluate the potential involvement of miR-145 in RA in vitro. The expression of miR-145 in the human fibroblast-like synoviocyte line MH7A was overexpressed by miR-mimic transfection, after which cells were subjected to lipopolysaccharides (LPS). Cell viability, apoptosis, and the release of pro-inflammatory cytokines were measured. The result showed that the apoptosis and the release of IL-1β, IL-6, IL-8, and TNF-α were significantly induced by LPS. Meanwhile, LPS treatment led to downregulation of miR-145. miR-145 overexpression in LPS-untreated MH7A cells had no impacts on cell apoptosis and inflammation. But, restoring miR-145 expression in LPS-stimulated cells by supplementation of a miR-145 mimic protected MH7A cells against LPS-induced apoptosis and inflammation. Furthermore, miR-145 overexpression in LPS-untreated MH7A cells slightly blocked the PI3K/ATK and mTOR pathways, whereas miR-145 overexpression in LPS-stimulated cells notably repressed the LPS-induced activation of PI3K/ATK and MAPK/mTOR pathways. Our study suggested that miR-145 protected MH7A cells against LPS-induced apoptosis and inflammation by inhibiting the PI3K/AKT and MAPK/mTOR pathways.

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“…miR‐145‐5p could suppress cell proliferation, invasion, and migration and induce apoptosis of CHL‐1 and VMM917 melanoma cells by inhibiting mitogen‐activated protein kinas (MAPK) and phosphatid‐ylinositol 3 kinase/protein kinae B (P13k/AKT) pathways . It might also function as a cardiac‐protective molecule in myocardial ischemic injury by ameliorating inflammation and apoptosis via negative regulation of CD40 .…”

Section: Discussionmentioning

confidence: 99%

MiR‐145 affected the circular RNA expression in prostate cancer LNCaP cells

Han

Zhou

et al. 2018

J of Cellular Biochemistry

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Recent evidence has demonstrated that circular RNAs (circRNAs) played crucial roles in fine-tuning the levels of gene expression by sequestering the corresponding microRNA (miRNAs). Their interaction with disease-associated miRNAs indicates that circRNAs are important for the development of disease, and miR-145 has been previously shown to have antitumor effect in prostate cancer. In the current study, we successfully established the miR-145-overexpressed prostate cancer LNCaP cells (LNCaP-miR-145) using lentiviral vectors. LNCaP cells expressing the empty vector (LNCaP-NC) were used as the negative control. The circRNA expression in LNCaP-miR-145 cells was detected by microarray analysis, and the miRNA targets of circRNAs were predicted using the bioinformatics software TargetScan and miRanda. Quantitative reverse transcription polymerase chain reaction was used to detect the expression levels of circRNAs in the prostate cancer tissue, nonmalignant tissue, LNCaP-miR-145 cells, and LNCaP-NC cells. The interaction of miRNA and circRNA was further confirmed by the dual-luciferase reporter assay. A total of 267 and 149 circRNAs were significantly up- and downregulated in LNCaP-miR-145 cells, respectively. hsa_circRNA_101981, hsa_circRNA_101996 and hsa_circRNA_09142 were the 3 circRNAs that interacted with hsa-miR-145-5p; hsa_circRNA_008068 and hsa_circRNA_406557 were the 2 circRNAs that interacted with hsa-miR-145-3p. Most of the circRNAs corresponded to the protein-coding exons. The expression levels of hsa_circRNA_101981, hsa_circRNA_00806, and hsa_circRNA_406557 were upregulated in the LNCaP-miR-145 cells, but downregulated in the prostate cancer tissue. In contrast, the expression levels of hsa_circRNA_101996 and hsa_circRNA_091420 were downregulated in the LNCaP-miR-145 cells, but upregulated in the prostate cancer tissue. Moreover, miR-145-5P might regulate the expression of hsa_circRNA_101981, hsa_circRNA_101996, and hsa_circRNA_09142, while miR-145-3P might regulate the expression of hsa_circRNA_008068 and hsa_circRNA_406557. Overexpression of miR-145 promoted the expression of hsa_circRNA_101981, hsa_circRNA_008068, and hsa_circRNA_406557 but suppressed the expressions of hsa_circRNA_101996 and hsa_circRNA_091420 in LNCaP cells. The results from the current study should give us a clue to clarify the tumor suppressive effect of miR-145.

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Retracted:Effects of miR‐145‐5p throughNRASon the cell proliferation, apoptosis, migration, and invasion in melanoma by inhibitingMAPKandPI3K/AKTpathways

Cited by 73 publications

References 45 publications

Circular RNA CEP128 promotes bladder cancer progression by regulating Mir‐145‐5p/Myd88 via MAPK signaling pathway

Circular RNA CEP128 promotes bladder cancer progression by regulating Mir‐145‐5p/Myd88 via MAPK signaling pathway

Retracted: miR‐145 eliminates lipopolysaccharides‐induced inflammatory injury in human fibroblast‐like synoviocyte MH7A cells

MiR‐145 affected the circular RNA expression in prostate cancer LNCaP cells

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